LOCUS       ___10_PB_CAG_L_b        8465 bp    DNA        circular     18-OCT-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 7, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
COMMENT     LOCUS GS56925 pBSK-seq56925 3680 bp DNA circular SYN 19-DEC-2014
            DEFINITION Ligation of inverted seq56925 into modified pBSK
            ACCESSION GS56925 pBSK-seq56925 KEYWORDS . SOURCE Unknown. ORGANISM
            Unknown Unclassified. REFERENCE 1 (bases 1 to 3680) AUTHORS Self
            JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager,
            Scientific & Educational Software COMMENT SECNOTES|Vector molecule:
            modified pBSK Fragment ends: SmaI Fragment size: 2848 Insert
            molecule: seq56925 Fragment ends: blunt Fragment size: 830
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             129..986
                     /locus_tag="Amp+"
                     /label="Amp+"
                     /ApEinfo_label="Amp+"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1137..1804)
                     /locus_tag="pUC origin"
                     /label="pUC origin"
                     /ApEinfo_label="pUC origin"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2200..2606)
                     /note="3' piggyBac TR"
                     /locus_tag="3' piggyBac TR"
                     /label="3' piggyBac TR"
                     /ApEinfo_label="3' piggyBac TR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     enhancer        2620..2997
                     /note="hCMV enhancer"
                     /locus_tag="hCMV enhancer"
                     /label="hCMV enhancer"
                     /ApEinfo_label="hCMV enhancer"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2943..2964)
                     /note="HN853"
                     /locus_tag="HN853"
                     /label="HN853"
                     /ApEinfo_label="HN853"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3003..3280
                     /note="Chicken b-actin promoter"
                     /locus_tag="Chicken b-actin promoter"
                     /label="Chicken b-actin promoter"
                     /ApEinfo_label="Chicken b-actin promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3190..3194
                     /note="CCATT box"
                     /locus_tag="CCATT box"
                     /label="CCATT box"
                     /ApEinfo_label="CCATT box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3252..3259
                     /note="TATA box"
                     /locus_tag="TATA box"
                     /label="TATA box"
                     /ApEinfo_label="TATA box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3264..3280
                     /note="TSS"
                     /locus_tag="TSS"
                     /label="TSS"
                     /ApEinfo_label="TSS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3281..3371
                     /note="Chicken b-actin exon 1"
                     /locus_tag="Chicken b-actin exon 1"
                     /label="Chicken b-actin exon 1"
                     /ApEinfo_label="Chicken b-actin exon 1"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3372..4296
                     /note="Chicken b-actin rabbit b-globin hybrid intron"
                     /locus_tag="Chicken b-actin rabbit b-globin hybrid intron"
                     /label="Chicken b-actin rabbit b-globin hybrid intron"
                     /ApEinfo_label="Chicken b-actin rabbit b-globin hybrid
                     intron"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3372..4247
                     /note="Chicken b-actin intron"
                     /locus_tag="Chicken b-actin intron"
                     /label="Chicken b-actin intron"
                     /ApEinfo_label="Chicken b-actin intron"
                     /ApEinfo_fwdcolor="#eb606c"
                     /ApEinfo_revcolor="#eb606c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4248..4344
                     /note="Rabbit b-globin splice acceptor"
                     /locus_tag="Rabbit b-globin splice acceptor"
                     /label="Rabbit b-globin splice acceptor"
                     /ApEinfo_label="Rabbit b-globin splice acceptor"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4261..4281
                     /note="HN737"
                     /locus_tag="HN737"
                     /label="HN737"
                     /ApEinfo_label="HN737"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4305..4324
                     /note="HN382"
                     /locus_tag="HN382"
                     /label="HN382"
                     /ApEinfo_label="HN382"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4356..4389)
                     /note="loxP"
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4390..4688
                     /note="bGH pA"
                     /locus_tag="bGH pA"
                     /label="bGH pA"
                     /ApEinfo_label="bGH pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4713..4940
                     /locus_tag="SV40 pA"
                     /label="SV40 pA"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4946..5173
                     /locus_tag="SV40 pA(1)"
                     /label="SV40 pA(1)"
                     /ApEinfo_label="SV40 pA(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5174..5184
                     /locus_tag="New STOP"
                     /label="New STOP"
                     /ApEinfo_label="New STOP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5191..5224)
                     /note="loxP"
                     /locus_tag="loxP(1)"
                     /label="loxP(1)"
                     /ApEinfo_label="loxP(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5234..6694
                     /note="tdGFP"
                     /locus_tag="tdGFP"
                     /label="tdGFP"
                     /ApEinfo_label="tdGFP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5234..5947
                     /note="superfolder GFP"
                     /locus_tag="superfolder GFP"
                     /label="superfolder GFP"
                     /ApEinfo_label="superfolder GFP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5948..5977
                     /note="linker"
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6726..6821
                     /note="mir-30a"
                     /locus_tag="mir-30a(1)"
                     /label="mir-30a(1)"
                     /ApEinfo_label="mir-30a(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6799..6804
                     /note="5' complement"
                     /locus_tag="5' complement"
                     /label="5' complement"
                     /ApEinfo_label="5' complement"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6843..6853
                     /note="5' basal stem"
                     /locus_tag="5' basal stem"
                     /label="5' basal stem"
                     /ApEinfo_label="5' basal stem"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6917..6927
                     /note="3' basal stem"
                     /locus_tag="3' basal stem"
                     /label="3' basal stem"
                     /ApEinfo_label="3' basal stem"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6933..7046
                     /note="mir-30a"
                     /locus_tag="mir-30a"
                     /label="mir-30a"
                     /ApEinfo_label="mir-30a"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6946..6951
                     /note="New EcoR1 site"
                     /locus_tag="New EcoR1 site"
                     /label="New EcoR1 site"
                     /ApEinfo_label="New EcoR1 site"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7101..7401
                     /note="bGH pA"
                     /locus_tag="bGH pA(1)"
                     /label="bGH pA(1)"
                     /ApEinfo_label="bGH pA(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(7408..7814)
                     /note="5' piggyBac TR"
                     /locus_tag="5' piggyBac TR"
                     /label="5' piggyBac TR"
                     /ApEinfo_label="5' piggyBac TR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7851..8006
                     /locus_tag="lacZ"
                     /label="lacZ"
                     /ApEinfo_label="lacZ"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(8139..8445)
                     /locus_tag="f1 origin"
                     /label="f1 origin"
                     /ApEinfo_label="f1 origin"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6854..6916
                     /locus_tag="shRNA sequence"
                     /label="shRNA sequence"
                     /ApEinfo_label="shRNA sequence"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacctccccc ggtattcacg acagcaggct
     2221 gaataataaa aaaattagaa actattattt aaccctagaa agataatcat attgtgacgt
     2281 acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg tatatcgagg
     2341 tttatttatt aatttgaata gatattaagt tttattatat ttacacttac atactaataa
     2401 taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa
     2461 aatttcttct ataaagtaac aaaactttta aacattctct cttttacaaa aataaactta
     2521 ttttgtactt taaaaacagt catgttgtat tataaaataa gtaattagct taacctatac
     2581 ataatagaaa caaattatac ttattaaccg gtcatggtcg acattgatta ttgactagtt
     2641 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     2701 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2761 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2821 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2881 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2941 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3001 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     3061 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
     3121 ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg
     3181 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
     3241 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc
     3301 cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     3361 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     3421 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
     3481 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     3541 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     3601 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     3661 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     3721 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc
     3781 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     3841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     3901 gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     3961 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     4021 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     4081 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     4141 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     4201 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     4261 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     4321 gtgctgtctc atcattttgg caaagaattg ctagcataac ttcgtatagc atacattata
     4381 cgaagttata gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4441 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4501 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4561 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4621 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcttgcgg
     4681 aacccttacc accgcggtgg cggccgctct agaatcataa tcagccatac cacatttgta
     4741 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     4801 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4921 aaactcatca atgtatctta actagaatca taatcagcca taccacattt gtagaggttt
     4981 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     5041 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5101 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5161 tcaatgtatc ttataactaa ataacctagg ataacttcgt atagcataca ttatacgaag
     5221 ttataggcgc gccatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt
     5281 tgaattagat ggtgatgtta atgggcacaa attttctgtc cgtggagagg gtgaaggtga
     5341 tgctacaaac ggaaaactca cccttaaatt tatttgcact actggaaaac tacctgttcc
     5401 gtggccaaca cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga
     5461 tcacatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg
     5521 cactatatct ttcaaagatg acgggaccta caagacgcgt gctgaagtca agtttgaagg
     5581 tgataccctt gttaatcgta tcgagttaaa gggtattgat tttaaagaag atggaaacat
     5641 tcttggacac aaactcgaat acaactttaa ctcacacaat gtatacatca cggcagacaa
     5701 acaaaagaat ggaatcaaag ctaacttcaa aattcgccac aacgttgaag atggttccgt
     5761 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     5821 agacaaccat tacctgtcga cacaatctgt cctttcgaaa gatcccaacg aaaagcgtga
     5881 ccacatggtc cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct
     5941 ctacaaaggt tctgctggat ctgcagcagg atctggtatg agcaaaggag aagaactttt
     6001 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     6061 tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg
     6121 cactactgga aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt
     6181 tcaatgcttt tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat
     6241 gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac
     6301 gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat
     6361 tgattttaaa gaagatggaa acattcttgg acacaaactc gaatacaact ttaactcaca
     6421 caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg
     6481 ccacaacgtt gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat
     6541 tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc
     6601 gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg
     6661 gattacacat ggcatggatg agctctacaa atgattaatt aatcgactag ggataacagg
     6721 gtaattgttt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca tcttggaaac
     6781 acttgctggg attacttcga cttcttaacc caacagaagg ctcgagaagg tatattgctg
     6841 ttgacagtga gcgCCAACCT CAGCAAGCTG ACTCATAGTG AAGCCACAGA TGTATGAGTC
     6901 AGCTTGCTGA GGTTGTtgcc tactgcctcg gacttcaagg ggctagaatt cgagcaatta
     6961 tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat
     7021 taaaatggta taaattaaat cactttcgat taatgatcat acagtgctct gagtacgcta
     7081 ggatccacta gagcggccgc ctagagctcg ctatcagcct cgactgtgcc ttctagttgc
     7141 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7201 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7261 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7321 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     7381 actagagctt gcggaaccct taagcttttt tattatatat tagtcacgat atctataaca
     7441 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
     7501 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
     7561 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
     7621 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
     7681 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
     7741 aaaagatttg cgctttactc gacctaaact ttaaacacgt catagaatct tcgtttgaca
     7801 aaaaccacat tgtgttgggt cgccctatag tgagtcgtat tacaattcac tggccgtcgt
     7861 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     7921 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7981 gttgcgcagc ctgaatggcg aatggcaaat tgtaagcgtt aatattttgt taaaattcgc
     8041 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8101 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     8161 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     8221 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     8281 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     8341 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     8401 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     8461 gtcag     
//
