LOCUS       Exported                7439 bp DNA     circular SYN 18-OCT-2021
DEFINITION  Ligation of ChR86-mCherry-12-(MVIBD-MBD)x2 into pAAV.hSynapsin.E 
            BamHI/HindIII.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Syn-ERT2-iCre-ERT2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Changyang Linghu
  TITLE     Direct Submission
  JOURNAL   Exported Oct 19, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     ExpressionRecording Islands (XRIs); Recording of cellular 
            physiological histories along optically readable self-assembling 
            protein chains
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          4816..4849
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    58..187
                     /label=ITR
                     /label=L-ITR
                     /note="L-ITR"
                     /note="color: #a6acb3"
     promoter        432..879
                     /label=Syn promoter
                     /note="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             897..1832
                     /codon_start=1
                     /product="mutated ligand-binding domain of the 
                     humanestrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /note="color: #993366"
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1842..2864
                     /codon_start=1
                     /label=iCre (condon-optimized)
                     /note="color: #993366"
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             2877..3812
                     /codon_start=1
                     /product="mutated ligand-binding domain of the 
                     humanestrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /note="color: #993366"
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     misc_feature    3843..4431
                     /label=WPRE
                     /note="WPRE"
                     /note="woodchuck 
                     hepatitisvirus?posttranscriptionalregulatory element"
                     /note="color: #a6acb3"
     polyA_signal    4470..4677
                     /label=bGH poly(A) signal
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    4686..4815
                     /label=ITR
                     /label=R-ITR
                     /note="R-ITR"
                     /note="color: #a6acb3"
     rep_origin      4924..5379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of 
                     replication;arrowindicates direction of (+) strand 
                     synthesis"
                     /note="color: #ffff00"
     promoter        5711..5815
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5816..6676
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments: 
                     ???1:?4783?..?4851?/?#ccffcc?/?signal?sequence 
                     ???2:?4852?..?5643?/?#ccffccCleavage site after base 4851"
                     /note="color: #99ccff"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6847..7435
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcct
       61 gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc gacctttggt
      121 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      181 ggttcctgcg gccgcacgcg ttctagaaat attaaggtac gggaggtact tggagcggcc
      241 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
      301 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
      361 tccccagtgc aagtgcaggt gccagaacat ttctctattc tagactcgag ctgcagaggg
      421 ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg gggtgcctac
      481 ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca aattgcgcat
      541 cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc actgccagct
      601 tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc
      661 actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc cggccacctt
      721 ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg cgagataggg
      781 gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg
      841 gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgaattcgcc accatggctg
      901 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
      961 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
     1021 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
     1081 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
     1141 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
     1201 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
     1261 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
     1321 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
     1381 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
     1441 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
     1501 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
     1561 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
     1621 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
     1681 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
     1741 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
     1801 tcacggggga ggcagagggt ttccctgcca cagctgtcga caacctgctg actgtgcacc
     1861 aaaacctgcc tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg
     1921 acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt
     1981 gcagatcctg ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg
     2041 aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc
     2101 aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg
     2161 actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg
     2221 agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga
     2281 tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca
     2341 acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg
     2401 atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg
     2461 tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg
     2521 gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg
     2581 ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc
     2641 accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc
     2701 actctgccag agtgggtgct gccagggaca tggccagggc tggtgtgtcc atccctgaaa
     2761 tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg
     2821 actctgagac tggggccatg gtgaggctgc tcgaggatgg ggacctcgag ccatctgctg
     2881 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
     2941 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
     3001 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
     3061 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
     3121 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
     3181 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
     3241 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
     3301 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
     3361 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
     3421 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
     3481 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
     3541 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
     3601 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
     3661 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
     3721 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
     3781 tcacggggga ggcagagggt ttccctgcca cagcttgagc tagcgatatc aagcttatcg
     3841 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3901 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3961 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4021 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4081 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4141 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4201 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4261 tcgcctgtgt tgccacctgg attctgcgcg ggacgccctt ctgctacgtc ccttcggccc
     4321 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4381 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     4441 gtcgactaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4501 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4561 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4621 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggagag
     4681 atctgaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     4741 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     4801 cgagcgagcg cgcagagagg gagtggcccc cccccccccc ccccccctgc agcctggcgt
     4861 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4921 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4981 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5041 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5101 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5161 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5221 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5281 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5341 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
     5401 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     5461 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5521 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5581 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5641 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5701 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5761 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5821 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5881 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5941 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6001 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6061 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6121 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6181 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6241 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6301 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6361 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6421 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6481 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6541 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6601 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6661 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6721 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6781 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6841 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6901 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6961 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7021 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7081 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7141 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7201 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7261 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7321 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7381 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacg
//
