LOCUS       ATP1A1_G3_EGFP_N        3006 bp    DNA     circular UNA 19-OCT-2021
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...8f-dzvvt1g
VERSION     urn.local...8f-dzvvt1g
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..241
                     /standard_name="Human U6 promoter"
     misc_RNA        250..270
                     /standard_name="EGFP nick"
     misc_feature    271..346
                     /note="chimeric guide RNA scaffold"
                     /note="Geneious type: misc_RNA"
                     /standard_name="sgRNA scaffold"
     misc_feature    347..352
                     /note="U6 terminator"
                     /note="Geneious type: terminator"
                     /standard_name="U6 terminator"
     promoter        465..705
                     /standard_name="Human U6 promoter"
     misc_feature    714..733
                     /note="Geneious type: misc_RNA"
                     /standard_name="ATP1A1 G3"
     misc_feature    734..809
                     /note="Geneious type: misc_RNA"
                     /standard_name="sgRNA scaffold"
     terminator      810..816
                     /standard_name="U6 Terminator"
     misc_feature    complement(848..869)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="M13R-20"
     misc_feature    complement(1054..1073)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pUC19 R"
     misc_feature    1237..1824
                     /note="Geneious type: rep_origin"
                     /standard_name="Ori"
     rep_origin      1237
     CDS             complement(1999..2856)
                     /standard_name="AmpR"
     misc_feature    complement(2857..2921)
                     /note="Geneious type: promoter"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ctcgtgacca ccctgaccta gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
      361 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
      421 gacaaatggc gtcgactgga tccggtacca aggtcgggca ggaagagggc ctatttccca
      481 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
      541 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
      601 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
      661 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgagttct
      721 gtaattcagc atagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      781 aacttgaaaa agtggcaccg agtcggtgct ttttttaagc ttggcgtaat catggtcata
      841 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      901 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      961 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1021 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1081 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1141 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1201 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1261 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1321 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1381 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1441 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1501 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1561 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1621 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     1681 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1741 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1801 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1861 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1921 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1981 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2041 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2101 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2161 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2221 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2281 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2341 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2401 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2461 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2521 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2581 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2641 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2701 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2761 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2821 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2881 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2941 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgctagctg
     3001 tacagt
//
