LOCUS       ATP1A1_G3_MTOR_N        3005 bp    DNA     circular UNA 19-OCT-2021
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...5c-dzvtsq8
VERSION     urn.local...5c-dzvtsq8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..241
                     /standard_name="Human U6 promoter"
     misc_RNA        250..269
                     /standard_name="MTOR Nick Exon-44"
     misc_feature    270..345
                     /note="chimeric guide RNA scaffold"
                     /note="Geneious type: misc_RNA"
                     /standard_name="sgRNA scaffold"
     misc_feature    346..351
                     /note="U6 terminator"
                     /note="Geneious type: terminator"
                     /standard_name="U6 terminator"
     promoter        464..704
                     /standard_name="Human U6 promoter"
     misc_feature    713..732
                     /note="Geneious type: misc_RNA"
                     /standard_name="ATP1A1 G3"
     misc_feature    733..808
                     /note="Geneious type: misc_RNA"
                     /standard_name="sgRNA scaffold"
     terminator      809..815
                     /standard_name="U6 Terminator"
     misc_feature    complement(847..868)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="M13R-20"
     misc_feature    complement(1053..1072)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pUC19 R"
     misc_feature    1236..1823
                     /note="Geneious type: rep_origin"
                     /standard_name="Ori"
     rep_origin      1236
     CDS             complement(1998..2855)
                     /standard_name="AmpR"
     misc_feature    complement(2856..2920)
                     /note="Geneious type: promoter"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg tggcatgaag gcctggaagg ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      421 acaaatggcg tcgactggat ccggtaccaa ggtcgggcag gaagagggcc tatttcccat
      481 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt
      541 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
      601 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
      661 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgagttctg
      721 taattcagca tagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
      781 acttgaaaaa gtggcaccga gtcggtgctt tttttaagct tggcgtaatc atggtcatag
      841 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1021 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1081 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     1681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1861 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1921 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1981 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2041 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2101 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2161 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2221 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2281 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2341 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2401 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2461 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2521 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2581 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2641 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2701 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2761 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2821 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2881 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2941 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgctagctgt
     3001 acagt
//
