LOCUS       ATP1A1_G8_Dual_s        3087 bp    DNA     circular UNA 19-OCT-2021
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...1f-dzvpwvz
VERSION     urn.local...1f-dzvpwvz
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        21..261
                     /standard_name="Human U6 promoter"
     misc_RNA        270..290
                     /standard_name="ATP1A1 G8"
     misc_RNA        291..366
                     /note="guide RNA scaffold for the <i>Streptococcus
                     pyogenes</i> CRISPR/Cas9 system"
                     /standard_name="gRNA scaffold"
     terminator      367..372
                     /standard_name="U6 terminator"
     promoter        485..725
                     /standard_name="Human U6 promoter"
     misc_feature    736..741
                     /standard_name="BbsI"
     misc_feature    744..749
                     /standard_name="BbsI"
     misc_RNA        752..827
                     /note="guide RNA scaffold for the <i>Streptococcus
                     pyogenes</i> CRISPR/Cas9 system"
                     /standard_name="gRNA scaffold"
     terminator      828..833
                     /standard_name="U6 terminator"
     misc_feature    949..970
                     /standard_name="M13R-20"
     misc_feature    1155..1174
                     /standard_name="pUC19 R"
     misc_feature    1338..1925
                     /note="vntifkey=""21"" - label=Ori - 867..1454"
                     /note="Geneious type: rep_origin"
                     /standard_name="Ori"
     rep_origin      1338
     CDS             complement(2100..2957)
                     /note="vntifkey=""4"" - label=AmpR -
                     complement(1629..2486)"
                     /standard_name="AmpR"
     misc_feature    complement(2958..3022)
                     /note="vntifkey=""21"" - label=bla\promoter - 2487..2551"
                     /note="Geneious type: promoter"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 gacgtcgcta gctgtacagt gagggcctat ttcccatgat tccttcatat ttgcatatac
       61 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
      121 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
      181 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
      241 tatatatctt gtggaaagga cgaaacaccg cagtgcccaa gtcaatgcag gttttagagc
      301 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      361 cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta
      421 gcgcgtgcgc caattctgca gacaaatggc gtcgactgga tccggtacca aggtcgggca
      481 ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
      541 agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
      601 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
      661 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      721 aggacgaaac accgggtctt cgagaagacc tgttttagag ctagaaatag caagttaaaa
      781 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag
      841 agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg ccaattctgc
      901 agacaaatgg ctctagtaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      961 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1021 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1081 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1141 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1201 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1261 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1321 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1381 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1441 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1501 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1561 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1621 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1681 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1741 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     1801 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1861 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1921 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1981 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2041 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2101 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2161 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2221 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2281 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2341 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2401 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2461 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2521 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2581 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2641 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2701 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2761 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2821 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2881 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2941 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3001 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3061 cgcacatttc cccgaaaagt gccacct
//
