LOCUS       ATP1A1_G8_MTOR_N        3089 bp    DNA     circular UNA 19-OCT-2021
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...2k-dzvs6hr
VERSION     urn.local...2k-dzvs6hr
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        21..261
                     /standard_name="Human U6 promoter"
     misc_RNA        270..290
                     /standard_name="ATP1A1 G8"
     misc_RNA        291..366
                     /note="guide RNA scaffold for the <i>Streptococcus
                     pyogenes</i> CRISPR/Cas9 system"
                     /standard_name="gRNA scaffold"
     terminator      367..372
                     /standard_name="U6 terminator"
     promoter        485..725
                     /standard_name="Human U6 promoter"
     misc_RNA        734..753
                     /standard_name="MTOR Nick Exon-53"
     misc_RNA        754..829
                     /note="guide RNA scaffold for the <i>Streptococcus
                     pyogenes</i> CRISPR/Cas9 system"
                     /standard_name="gRNA scaffold"
     terminator      830..835
                     /standard_name="U6 terminator"
     misc_feature    951..972
                     /standard_name="M13R-20"
     misc_feature    1157..1176
                     /standard_name="pUC19 R"
     misc_feature    1340..1927
                     /note="vntifkey=""21"" - label=Ori - 867..1454"
                     /note="Geneious type: rep_origin"
                     /standard_name="Ori"
     rep_origin      1340
     CDS             complement(2102..2959)
                     /note="vntifkey=""4"" - label=AmpR -
                     complement(1629..2486)"
                     /standard_name="AmpR"
     misc_feature    complement(2960..3024)
                     /note="vntifkey=""21"" - label=bla\promoter - 2487..2551"
                     /note="Geneious type: promoter"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 gacgtcgcta gctgtacagt gagggcctat ttcccatgat tccttcatat ttgcatatac
       61 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
      121 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
      181 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
      241 tatatatctt gtggaaagga cgaaacaccg cagtgcccaa gtcaatgcag gttttagagc
      301 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      361 cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta
      421 gcgcgtgcgc caattctgca gacaaatggc gtcgactgga tccggtacca aggtcgggca
      481 ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
      541 agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
      601 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
      661 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      721 aggacgaaac accgcagcac ctccatcact gtggttttag agctagaaat agcaagttaa
      781 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt
      841 agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct
      901 gcagacaaat ggctctagta agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      961 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1021 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1081 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1141 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1201 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1261 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1321 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1381 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1441 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1501 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1561 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1621 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1681 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1741 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     1801 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1861 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1981 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2041 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2101 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2161 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2221 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2281 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2341 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2401 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2461 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2521 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2581 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2641 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2701 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2761 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2821 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2881 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2941 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3001 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3061 cgcgcacatt tccccgaaaa gtgccacct
//
