LOCUS       pPC1551                 5765 bp ds-DNA     circular SYN 28-MAY-2021
DEFINITION  [pBR322, Cb] pCMV-pT7-hMLH1dn genscript mRNA for IVT.
ACCESSION
VERSION     .
KEYWORDS    pCMV-PE2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 9753)
  TITLE     Liu Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 18, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        1..380
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        381..584
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     535..555
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     regulatory      complement(583..605)
                     /created_by="everette"
                     /label="VECTOR PRIMER 1"
     misc_feature    606..677
                     /Original_Bases="CGCTAGAGATCCGCGGCCGCTAATACGACTCACTATAGGGA
                     GAGCC"
                     /label="CGCTAGAGATCCGCGGCCGCTAATACGACTCACTATAGGGAGAGCC"
                     /note="Geneious type: Editing History Replacement"
     regulatory      complement(606..661)
                     /created_by="everette"
                     /label="VECTOR PRIMER 1"
     primer_bind     606..639
                     /%_Identity="97.05882352941177"
                     /Motif="TCGAGCTCGGTACCTAATACGACTCACTATAAGG"
                     /Sequence="TCGAGCTCGGTACCTAATACGACACACTATAAGG"
                     /Tm="67.8"
                     /%GC="47.1"
                     /Hairpin_Tm="40.3"
                     /Self_Dimer_Tm="34.0"
                     /modified_by="chenp"
                     /label="IVT primer forward"
     misc_RNA        620..683
                     /created_by="nicolegaudelli"
                     /label="5'UTR sequence"
     promoter        620..639
                     /created_by="nicolegaudelli"
                     /label="T7 promoter with sequence mismatch"
     mismatch        629
                     /Motif="TCGAGCTCGGTACCTAATACGACTCACTATAAGG"
                     /annotation_group="TCGAGCTCGGTACCTAATACGACTCACTATAAGG: 606
                     -> 639"
                     /label="T"
     primer_bind     complement(648..691)
                     /Sequence="AAGCTCATGGTGGCTCTTATATTTCTTCTTACTCTTCTTTTCTC"
                     /Tm="61.9"
                     /%GC="36.4"
                     /Hairpin_Tm="34.0"
                     /Self_Dimer_Tm="5.0"
                     /created_by="chenp"
                     /label="PC1712"
     regulatory      652..683
                     /created_by="everette"
                     /label="EDITOR PRIMER 1"
     primer_bind     681..709
                     /Sequence="ACCATGAGCTUCGTTGCTGGAGTCATCCG"
                     /%GC="55.2"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1366"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:de-cczyqea"">PC1366</a>"
                     /label="PC1366"
     CDS             684..2942
                     /created_by="chenp"
                     /modified_by="chenp"
                     /label="MLH1 del754-756 genscript codon"
     misc_feature    688..3046
                     /Original_Bases="AACGGACAGCCGACGGAAGCGAGTTCGAGTCACCAAAGAAG
                     AAGCGGAAAGTCGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACTCTGTGG
                     GCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGG
                     CAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGC
                     GGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGAC
                     GGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGA
                     CGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCAC
                     GAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACC
                     CCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGCG
                     GCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAG
                     GGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGA
                     CCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGC
                     CATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCAGCTG
                     CCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGA
                     CCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAA
                     GGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCC
                     GACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGA
                     GAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGA
                     CGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAG
                     AAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATTGACG
                     GCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGA
                     CGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGG
                     ACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCCATTC
                     TGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAA
                     GATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGA
                     TTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAGGAAG
                     TGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAA
                     GAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACC
                     GTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCT
                     TCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAA
                     AGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCC
                     GTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCACGATC
                     TGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCT
                     GGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGG
                     CTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGA
                     GATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCA
                     GTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTC
                     ATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGG
                     TGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGC
                     CATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATG
                     GGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCACCC
                     AGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGA
                     GCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAG
                     AAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACTGG
                     ACATCAACCGGCTGTCCGACTACGATGTGGACGCTATCGTGCCTCAGAGCTTTCTGAA
                     GGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGC
                     GACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGC
                     TGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGG
                     CGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGG
                     CAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACG
                     AGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTC
                     CGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCAC
                     GCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTA
                     AGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGAT
                     CGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAAC
                     ATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGCGGC
                     CTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTT
                     TGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAG
                     GTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGC
                     TGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCAC
                     CGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTG
                     AAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGA
                     ATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCAT
                     CAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCC
                     TCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACT
                     TCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCA
                     GAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATC
                     AGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCG
                     CCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCT
                     GTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATC
                     GACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGA
                     GCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGTGACTCTGG
                     AGGATCTAGCGGAGGATCCTCTGGCAGCGAGACACCAGGAACAAGCGAGTCAGCAACA
                     CCAGAGAGCAGTGGCGGCAGCAGCGGCGGCAGCAGCACCCTAAATATAGAAGATGAGT
                     ATCGGCTACATGAGACCTCAAAAGAGCCAGATGTTTCTCTAGGGTCCACATGGCTGTC
                     TGATTTTCCTCAGGCCTGGGCGGAAACCGGGGGCATGGGACTGGCAGTTCGCCAAGCT
                     CCTCTGATCATACCTCTGAAAGCAACCTCTACCCCCGTGTCCATAAAACAATACCCCA
                     TGTCACAAGAAGCCAGACTGGGGATCAAGCCCCACATACAGAGACTGTTGGACCAGGG
                     AATACTGGTACCCTGCCAGTCCCCCTGGAACACGCCCCTGCTACCCGTTAAGAAACCA
                     GGGACTAATGATTATAGGCCTGTCCAGGATCTGAGAGAAGTCAACAAGCGGGTGGAAG
                     ACATCCACCCCACCGTGCCCAACCCTTACAACCTCTTGAGCGGGCTCCCACCGTCCCA
                     CCAGTGGTACACTGTGCTTGATTTAAAGGATGCCTTTTTCTGCCTGAGACTCCACCCC
                     ACCAGTCAGCCTCTCTTCGCCTTTGAGTGGAGAGATCCAGAGATGGGAATCTCAGGAC
                     AATTGACCTGGACCAGACTCCCACAGGGTTTCAAAAACAGTCCCACCCTGTTTAATGA
                     GGCACTGCACAGAGACCTAGCAGACTTCCGGATCCAGCACCCAGACTTGATCCTGCTA
                     CAGTACGTGGATGACTTACTGCTGGCCGCCACTTCTGAGCTAGACTGCCAACAAGGTA
                     CTCGGGCCCTGTTACAAACCCTAGGGAACCTCGGGTATCGGGCCTCGGCCAAGAAAGC
                     CCAAATTTGCCAGAAACAGGTCAAGTATCTGGGGTATCTTCTAAAAGAGGGTCAGAGA
                     TGGCTGACTGAGGCCAGAAAAGAGACTGTGATGGGGCAGCCTACTCCGAAGACCCCTC
                     GACAACTAAGGGAGTTCCTAGGGAAGGCAGGCTTCTGTCGCCTCTTCATCCCTGGGTT
                     TGCAGAAATGGCAGCCCCCCTGTACCCTCTCACCAAACCGGGGACTCTGTTTAATTGG
                     GGCCCAGACCAACAAAAGGCCTATCAAGAAATCAAGCAAGCTCTTCTAACTGCCCCAG
                     CCCTGGGGTTGCCAGATTTGACTAAGCCCTTTGAACTCTTTGTCGACGAGAAGCAGGG
                     CTACGCCAAAGGTGTCCTAACGCAAAAACTGGGACCTTGGCGTCGGCCGGTGGCCTAC
                     CTGTCCAAAAAGCTAGACCCAGTAGCAGCTGGGTGGCCCCCTTGCCTACGGATGGTAG
                     CAGCCATTGCCGTACTGACAAAGGATGCAGGCAAGCTAACCATGGGACAGCCACTAGT
                     CATTCTGGCCCCCCATGCAGTAGAGGCACTAGTCAAACAACCCCCCGACCGCTGGCTT
                     TCCAACGCCCGGATGACTCACTATCAGGCCTTGCTTTTGGACACGGACCGGGTCCAGT
                     TCGGACCGGTGGTAGCCCTGAACCCGGCTACGCTGCTCCCACTGCCTGAGGAAGGGCT
                     GCAACACAACTGCCTTGATATCCTGGCCGAAGCCCACGGAACCCGACCCGACCTAACG
                     GACCAGCCGCTCCCAGACGCCGACCACACCTGGTACACGGATGGAAGCAGTCTCTTAC
                     AAGAGGGACAGCGTAAGGCGGGAGCTGCGGTGACCACCGAGACCGAGGTAATCTGGGC
                     TAAAGCCCTGCCAGCCGGGACATCCGCTCAGCGGGCTGAACTGATAGCACTCACCCAG
                     GCCCTAAAGATGGCAGAAGGTAAGAAGCTAAATGTTTATACTGATAGCCGTTATGCTT
                     TTGCTACTGCCCATATCCATGGAGAAATATACAGAAGGCGTGGGTGGCTCACATCAGA
                     AGGCAAAGAGATCAAAAATAAAGACGAGATCTTGGCCCTACTAAAAGCCCTCTTTCTG
                     CCCAAAAGACTTAGCATAATCCATTGTCCAGGACATCAAAAGGGACACAGCGCCGAGG
                     CTAGAGGCAACCGGATGGCTGACCAAGCGGCCCGAAAGGCAGCCATCACAGAGACTCC
                     AGACACCTCTACCCTCCTCATAGAAAATTCATCACCCTCTGGCGGCTCAAAAAGAACC
                     GCCGACGGCAGCGAATTCGAGCCCAAGAAGAAGAGGAAAGTCTAACCGGTCATCATCA
                     CCATCACCAT"
                     /label="AACGGACAGCCGACGGAAGCGAGTTCGAGTCACCAAAGAAGAAGCGGAAA
                     GTCGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCG
                     TGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGA
                     CCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACA
                     GCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGACGGAAGAACC
                     GGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTT
                     CTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCAC
                     CCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCT
                     ACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTA
                     TCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTG
                     AACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACC
                     AGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTC
                     TGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAG
                     AAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGACCCCCAACT
                     TCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTA
                     CGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTT
                     CTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAGAGTGAACA
                     CCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGACGAGCACCA
                     CCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAAGTACAAA
                     GAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATTGACGGCGGAGCCA
                     GCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACGGCACCGA
                     GGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGAC
                     AACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCGGCGGC
                     AGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCCTGAC
                     CTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGG
                     ATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAGGAAGTGGTGGACA
                     AGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAACCTGCC
                     CAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAAC
                     GAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCG
                     GCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGT
                     GAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATC
                     TCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAA
                     TTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGATAT
                     CGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACC
                     TATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCG
                     GCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAA
                     GACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTG
                     ATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCC
                     AGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAA
                     GGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCAC
                     AAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCACCCAGAAGGGAC
                     AGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAG
                     CCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTAC
                     CTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACTGGACATCAACC
                     GGCTGTCCGACTACGATGTGGACGCTATCGTGCCTCAGAGCTTTCTGAAGGACGACTC
                     CATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTG
                     CCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCA
                     AGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAG
                     CGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACA
                     AAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACGAGAATGACA
                     AGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCG
                     GAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGAC
                     GCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTAAGCTGGAAA
                     GCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAG
                     CGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAAC
                     TTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGCGGCCTCTGATCG
                     AGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGT
                     GCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACA
                     GGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCA
                     GAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTA
                     TTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTG
                     AAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCG
                     ACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCC
                     TAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGC
                     GAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACC
                     TGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCT
                     GTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTC
                     TCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACA
                     AGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCT
                     GACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAG
                     AGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCG
                     GCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGTGACTCTGGAGGATCTAG
                     CGGAGGATCCTCTGGCAGCGAGACACCAGGAACAAGCGAGTCAGCAACACCAGAGAGC
                     AGTGGCGGCAGCAGCGGCGGCAGCAGCACCCTAAATATAGAAGATGAGTATCGGCTAC
                     ATGAGACCTCAAAAGAGCCAGATGTTTCTCTAGGGTCCACATGGCTGTCTGATTTTCC
                     TCAGGCCTGGGCGGAAACCGGGGGCATGGGACTGGCAGTTCGCCAAGCTCCTCTGATC
                     ATACCTCTGAAAGCAACCTCTACCCCCGTGTCCATAAAACAATACCCCATGTCACAAG
                     AAGCCAGACTGGGGATCAAGCCCCACATACAGAGACTGTTGGACCAGGGAATACTGGT
                     ACCCTGCCAGTCCCCCTGGAACACGCCCCTGCTACCCGTTAAGAAACCAGGGACTAAT
                     GATTATAGGCCTGTCCAGGATCTGAGAGAAGTCAACAAGCGGGTGGAAGACATCCACC
                     CCACCGTGCCCAACCCTTACAACCTCTTGAGCGGGCTCCCACCGTCCCACCAGTGGTA
                     CACTGTGCTTGATTTAAAGGATGCCTTTTTCTGCCTGAGACTCCACCCCACCAGTCAG
                     CCTCTCTTCGCCTTTGAGTGGAGAGATCCAGAGATGGGAATCTCAGGACAATTGACCT
                     GGACCAGACTCCCACAGGGTTTCAAAAACAGTCCCACCCTGTTTAATGAGGCACTGCA
                     CAGAGACCTAGCAGACTTCCGGATCCAGCACCCAGACTTGATCCTGCTACAGTACGTG
                     GATGACTTACTGCTGGCCGCCACTTCTGAGCTAGACTGCCAACAAGGTACTCGGGCCC
                     TGTTACAAACCCTAGGGAACCTCGGGTATCGGGCCTCGGCCAAGAAAGCCCAAATTTG
                     CCAGAAACAGGTCAAGTATCTGGGGTATCTTCTAAAAGAGGGTCAGAGATGGCTGACT
                     GAGGCCAGAAAAGAGACTGTGATGGGGCAGCCTACTCCGAAGACCCCTCGACAACTAA
                     GGGAGTTCCTAGGGAAGGCAGGCTTCTGTCGCCTCTTCATCCCTGGGTTTGCAGAAAT
                     GGCAGCCCCCCTGTACCCTCTCACCAAACCGGGGACTCTGTTTAATTGGGGCCCAGAC
                     CAACAAAAGGCCTATCAAGAAATCAAGCAAGCTCTTCTAACTGCCCCAGCCCTGGGGT
                     TGCCAGATTTGACTAAGCCCTTTGAACTCTTTGTCGACGAGAAGCAGGGCTACGCCAA
                     AGGTGTCCTAACGCAAAAACTGGGACCTTGGCGTCGGCCGGTGGCCTACCTGTCCAAA
                     AAGCTAGACCCAGTAGCAGCTGGGTGGCCCCCTTGCCTACGGATGGTAGCAGCCATTG
                     CCGTACTGACAAAGGATGCAGGCAAGCTAACCATGGGACAGCCACTAGTCATTCTGGC
                     CCCCCATGCAGTAGAGGCACTAGTCAAACAACCCCCCGACCGCTGGCTTTCCAACGCC
                     CGGATGACTCACTATCAGGCCTTGCTTTTGGACACGGACCGGGTCCAGTTCGGACCGG
                     TGGTAGCCCTGAACCCGGCTACGCTGCTCCCACTGCCTGAGGAAGGGCTGCAACACAA
                     CTGCCTTGATATCCTGGCCGAAGCCCACGGAACCCGACCCGACCTAACGGACCAGCCG
                     CTCCCAGACGCCGACCACACCTGGTACACGGATGGAAGCAGTCTCTTACAAGAGGGAC
                     AGCGTAAGGCGGGAGCTGCGGTGACCACCGAGACCGAGGTAATCTGGGCTAAAGCCCT
                     GCCAGCCGGGACATCCGCTCAGCGGGCTGAACTGATAGCACTCACCCAGGCCCTAAAG
                     ATGGCAGAAGGTAAGAAGCTAAATGTTTATACTGATAGCCGTTATGCTTTTGCTACTG
                     CCCATATCCATGGAGAAATATACAGAAGGCGTGGGTGGCTCACATCAGAAGGCAAAGA
                     GATCAAAAATAAAGACGAGATCTTGGCCCTACTAAAAGCCCTCTTTCTGCCCAAAAGA
                     CTTAGCATAATCCATTGTCCAGGACATCAAAAGGGACACAGCGCCGAGGCTAGAGGCA
                     ACCGGATGGCTGACCAAGCGGCCCGAAAGGCAGCCATCACAGAGACTCCAGACACCTC
                     TACCCTCCTCATAGAAAATTCATCACCCTCTGGCGGCTCAAAAAGAACCGCCGACGGC
                     AGCGAATTCGAGCCCAAGAAGAAGAGGAAAGTCTAACCGGTCATCATCACCATCACCA
                     T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(755..790)
                     /Sequence="ATCATCTCCTTTATTGCATTGGCAGGTCTCTGGATC"
                     /Tm="69.0"
                     /%GC="44.4"
                     /Hairpin_Tm="53.1"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1365"
     USER            777..790
                     /created_by="chenp"
     primer_bind     777..809
                     /Sequence="ATAAAGGAGATGATCGAGAACTGTCTGGACGCC"
                     /Tm="68.7"
                     /%GC="48.5"
                     /Hairpin_Tm="40.9"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1364"
     primer_bind     1261..1283
                     /Sequence="GCGTAAAGAAGCAGGGAGAAACC"
                     /Tm="62.0"
                     /%GC="52.2"
                     /Hairpin_Tm="38.1"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1473"
     primer_bind     1262..1284
                     /Sequence="CGTAAAGAAGCAGGGAGAAACCG"
                     /Tm="61.7"
                     /%GC="52.2"
                     /Hairpin_Tm="40.1"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1418"
     primer_bind     1925..1947
                     /Sequence="GGACAAGACAGATATCTCCTCCG"
                     /Tm="59.7"
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.0"
                     /created_by="chenp"
                     /label="PC1419"
     primer_bind     2644..2664
                     /Sequence="CCATCTTCATCCTGAGACTGG"
                     /Tm="57.5"
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1472"
     primer_bind     complement(2910..2947)
                     /Sequence="AATTAGAAAACCTTATAAAGGTCGGGCAGGTTGGCCAG"
                     /Tm="64.3"
                     /%GC="44.7"
                     /Hairpin_Tm="54.6"
                     /Self_Dimer_Tm="16.7"
                     /created_by="chenp"
                     /label="PC1714"
     USER            2932..2947
                     /created_by="chenp"
     primer_bind     2932..2968
                     /Sequence="ATAAGGTTTTCTAATTAATTAAGCTGCCTTCTGCGGG"
                     /Tm="60.8"
                     /%GC="37.8"
                     /Hairpin_Tm="43.5"
                     /Self_Dimer_Tm="17.6"
                     /created_by="chenp"
                     /label="PC1713"
     misc_feature    complement(2946..3046)
                     /created_by="Alexander Sousa"
                     /modified_by="Alexander Sousa"
                     /label="BEAM 3' UTR"
     primer_bind     2983..3069
                     /created_by="Alexander Sousa"
                     /Sequence="CATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAAT
                     AAAGCCTGAGTAGGAAGTGAGTTTAAACCCGCTGATCAGC"
                     /Tm="81.6"
                     /%GC="48.3"
                     /modified_by="Alexander Sousa"
                     /label="LoAS4046"
     primer_bind     complement(3018..3046)
                     /created_by="chenp"
                     /Sequence="CTTCCTACTCAGGCTTTATTCAAAGACCA"
                     /Tm="62.9"
                     /%GC="41.4"
                     /Hairpin_Tm="49.2"
                     /Self_Dimer_Tm="None"
                     /modified_by="chenp"
                     /label="IVT primer reverse"
     polyA_signal    3075..3299
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     misc_feature    3176
                     /label="mut"
     primer_bind     complement(3370..3386)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     protein_bind    3394..3410
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3418..3448)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3463..3484
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3772..4360)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(4057..4085)
                     /Sequence="ACTCAAGACGAUAGTTACCGGATAAGGCG"
                     /%GC="48.3"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0970"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:2e-b64q18g"">PC0970</a>"
                     /label="PC0970"
     primer_bind     4074..4100
                     /Sequence="ATCGTCTTGAGUCCAACCCGGTAAGAC"
                     /%GC="51.9"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0969"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-b64q18e"">PC0969</a>"
                     /label="PC0969"
     CDS             complement(4531..5391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     5154..5173
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(5392..5496)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     complement(5575..5594)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
ORIGIN      
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatctcgag ctcggtacct aatacgacac actataagga aataagagag aaaagaagag
      661 taagaagaaa tataagagcc accatgagct tcgttgctgg agtcatccgg agactggacg
      721 agacagtggt gaacagaatt gccgccggcg aggtgatcca gagacctgcc aatgcaataa
      781 aggagatgat cgagaactgt ctggacgcca agtccacaag cattcaggtg atcgtgaagg
      841 agggcggact gaagctgatc cagatccaag acaacggcac aggcatcaga aaggaagatc
      901 tggacatcgt gtgtgaacgg ttcaccacat ctaagctgca gtcttttgag gatctggcct
      961 ctatcagtac ctacggcttc agaggcgagg ccctggccag catcagccac gtggcccatg
     1021 tgaccatcac caccaaaacc gccgacggca aatgcgctta tcgcgctagc tacagcgacg
     1081 gcaagctgaa agccccgcca aagccttgcg ccggcaacca gggtacacag ataacagtgg
     1141 aggatctgtt ctacaacatc gccacccgga gaaaggccct gaaaaatccc agcgaggagt
     1201 acggcaagat cctggaagtc gtcggcagat actccgtgca caacgccgga atcagcttta
     1261 gcgtaaagaa gcagggagaa accgtggccg atgtgcgcac cctgccaaat gccagcaccg
     1321 tggataacat cagaagcatt ttcggaaatg ccgtgtccag agaactgatc gagatcggct
     1381 gcgaagataa gaccctggct tttaagatga acggctacat cagcaacgcc aattactctg
     1441 tgaagaagtg catctttctt ctgttcatca accacagact ggtggaaagc accagcctgc
     1501 ggaaagccat cgagacagtg tacgccgcct acctgcctaa gaacacccac cccttcctgt
     1561 acctgagcct cgagatcagc cctcagaacg tggacgtcaa tgtgcatcct acaaagcacg
     1621 aggtgcactt cctgcacgag gaaagcatcc tggaaagagt gcagcagcac attgagagca
     1681 agctgctggg ctctaacagc agcagaatgt acttcacaca gaccctgctg cctggcctgg
     1741 ccggcccctc aggcgaaatg gttaagtcca caacctctct gacctcatcc agcaccagcg
     1801 gttcttccga taaggtgtac gcccaccaga tggtgcggac cgactctcgg gagcagaagc
     1861 tggacgcctt tctgcaacct ctgagcaaac ctctgagctc tcagcctcag gccatcgtga
     1921 ccgaggacaa gacagatatc tcctccggcc gtgccagaca gcaggacgaa gaaatgctcg
     1981 agctgccagc tcctgccgag gtggccgcca agaaccagag cctggaggga gataccacaa
     2041 agggcaccag cgaaatgagc gagaagcggg gccctacctc cagcaacccc agaaaacggc
     2101 accgggagga cagcgacgtg gaaatggtgg aggacgacag cagaaaggaa atgacagccg
     2161 cttgtacccc tagaagaaga atcatcaacc tgacctccgt gctgagcctg caggaggaga
     2221 tcaacgagca gggccacgag gtgctgagag agatgctgca caatcacagc ttcgtgggct
     2281 gcgtgaaccc tcaatgggcc ctggctcagc atcaaacaaa gctgtacctg ctgaacacca
     2341 ccaagctgag cgaagagctg ttctaccaga tcctcatcta cgacttcgcc aacttcggcg
     2401 tgctacgcct gagcgagccc gcccctctgt ttgacctggc catgctggct ctggatagcc
     2461 cagaaagcgg ctggacagaa gaggacggac ctaaagaggg gctggctgaa tacatcgtgg
     2521 agttcctgaa gaaaaaggcc gagatgctgg ccgactactt ttctctggaa atcgacgagg
     2581 aaggcaacct gatcggcctg cctctgctga tcgataacta cgtgcctccc ctggaaggcc
     2641 tgcccatctt catcctgaga ctggctacag aggtgaactg ggacgaggaa aaggaatgct
     2701 tcgagtctct gagcaaggag tgcgccatgt tctatagcat cagaaaacag tacatctctg
     2761 aagagagcac tctgtctggc cagcagagtg aagtgcccgg aagcatcccc aacagctgga
     2821 agtggaccgt ggaacacatc gtgtacaagg ccctgcggag ccacattctc cctcctaagc
     2881 acttcaccga ggacggcaac atcctgcagc tggccaacct gcccgacctt tataaggttt
     2941 tctaattaat taagctgcct tctgcggggc ttgccttctg gccatgccct tcttctctcc
     3001 cttgcacctg tacctcttgg tctttgaata aagcctgagt aggaagtgag tttaaacccg
     3061 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     3121 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgagaaaat
     3181 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     3241 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     3301 ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct agagcttggc
     3361 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3421 catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga gctaactcac
     3481 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3541 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4201 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4381 tacggggtct gacactcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4441 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4501 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4561 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4621 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4681 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4741 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4801 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4861 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4921 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4981 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5041 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5101 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5161 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5221 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5281 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5341 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5401 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5461 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5521 tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtctactc tcagtacaat
     5581 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     5641 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     5701 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     5761 gcgtt
//
