LOCUS       pKAN43Ag8-GFP_fo       10952 bp DNA     circular SYN 17-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    544..568
                     /label=LB T-DNA repeat
                     /note="left border repeat from pRiA4"
     promoter        1171..1350
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    1387..1464
                     /label=AtAGP21 5'UTR
                     /note="5′-untranslated region of the Arabidopsis thaliana 
                     arabinogalactan-protein 21 gene. Mtsui et al., Plant 
                     Biotechnology 29, 319–322 (2012)"
     CDS             1468..2265
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2322..2496
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     enhancer        join(2599..2937,2938..3276,3277..3615,3616..3954)
                     /label=4xCaMV35S
                     /note="tetramer of cauliflower mosaic virus 35S enhancer."
     promoter        3988..4034
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    4051..4128
                     /label=AtAGP215'UTR
                     /note="5′-untranslated region of the Arabidopsis thaliana 
                     arabinogalactan-protein 21 gene. Mtsui et al., Plant 
                     Biotechnology 29, 319–322 (2012)"
     CDS             join(4132..4134,4135..4137,4138..4851)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MHSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     misc_feature    4860..5738
                     /label=HSPT878
                     /note="The longer version of Arabidopsis thaliana heat 
                     shock protein 18.2 gene terminator. Matsui et al. 2014 
                     Plant Biotechnology 31(2):191-194
                     "
     misc_feature    5787..5811
                     /label=RB T-DNA repeat
                     /note="right border repeat from pRiA4"
     promoter        6312..6416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6417..6485,6486..7277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7448..8036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      8234..8428
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8494..9561)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    complement(9995..10865)
                     /label=pVS1 staA -NdeI
                     /note="Stability protein from Pseudomonas plasmid pVS1. 
                     NdeI site is deleted."
ORIGIN
        1 ctcgagcttg gttcgagccc atattctgat caccatagcg ccgaaggcgc tttcacaggt
       61 gacggcctcc cttgggaagg tcacggccgt tatcgctctt cattctgtga gtggcacttt
      121 tgatttgatt gcgattatcg aagccccttc gatttcagag ctcgatcagt tcatcgatca
      181 aatcggcata attgaaggtg tcgagcggac gctctcttcg attattcttt caacacgcat
      241 ttcacgctga aagagcaaga ggccacagcc gtgcgcctgc tgccaccttt ccgacgatga
      301 ccatgcagaa cttacggcct ctatgtcggt gtattccacc caccgacgcg ccatttccgt
      361 gtgcattaag ataatgcttt ccgccccatc ggattgctgt tctgacatgg tatatcatta
      421 cgacagccaa tgtttggcgg ctcgtacgaa tcatcaagcg gtcaacctgt gctgtcaacc
      481 gtcacgatat gagcaagcaa ctcaaacgct ttcctattca ccgcacgcgc gttgttggct
      541 gtttggcagg atatatgcca acgtaaaaat gagggcaatc gattgtactg aatcggattt
      601 tcaagggtct ggccaaaact attccgtggg cacctggcac acgccctgga gtccggcccg
      661 tttccagttg agggttgtct acgcttagat gagaaggaaa gttgtccaag acgaatccca
      721 gtgtcctatt accaatagcc gacggtatcg ataagcttat cgacggatca gcttcacgct
      781 gccgcaagca ctcaggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
      841 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
      901 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
      961 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
     1021 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     1081 ggatcaagat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
     1141 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
     1201 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     1261 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
     1321 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccagatct
     1381 gatcccatca tcacaacaca aatcaaaaca agaataacaa aatctttctc ttataaattc
     1441 ttatttcaag acatcaaagg agaaaccatg gggattgaac aagatggatt gcacgcaggt
     1501 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     1561 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     1621 accgacctgt ccggtgccct gaatgaactc caggacgagg cagcgcggct atcgtggctg
     1681 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     1741 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     1801 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     1861 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     1921 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     1981 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac acatggcgat
     2041 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     2101 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     2161 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     2221 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     2281 tcggatcgat cctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt
     2341 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
     2401 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     2461 ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattg gtaccggcct
     2521 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2581 tttgagtgag ctggatcccc aacatggtgg agcacgacac tctcgtctac tccaagaata
     2641 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
     2701 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
     2761 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
     2821 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
     2881 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tagatcccca
     2941 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     3001 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     3061 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
     3121 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
     3181 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     3241 cttcaaagca agtggattga tgtgatatct agatccccaa catggtggag cacgacactc
     3301 tcgtctactc caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt
     3361 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     3421 tcatcgaaag gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag
     3481 gaaaggctat cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca
     3541 cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     3601 gtgatatcta gatccccaac atggtggagc acgacactct cgtctactcc aagaatatca
     3661 aagatacagt ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg
     3721 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa
     3781 aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     3841 cctctaccga cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag
     3901 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctag atccgaaact
     3961 atcagtgttt gacagaaggg cgaattcgca agacccttcc tctatataag gaagttcatt
     4021 tcatttggag agaacacggg ggactctaga atcatcacaa cacaaatcaa aacaagaata
     4081 acaaaatctt tctcttataa attcttattt caagacatca aaggagaaaa gatgcatagc
     4141 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4201 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4261 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4321 accttcacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     4381 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4441 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4501 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     4561 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     4621 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     4681 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     4741 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     4801 ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaagta aggcgcgcca
     4861 atatgaagat gaagatgaaa tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt
     4921 gtgttttttt cttggcttgt tgtgttatga atttgtggct ttttctaata ttaaatgaat
     4981 gtaagatctc attataatga ataaacaaat gtttctataa tccattgtga atgttttgtt
     5041 ggatctcttc tgcagcatat aactactgta tgtgctatgg tatggactat ggaatatgat
     5101 taaagataag atgggctcat agagtaaaac gaggcgaggg acctataaac ctcccttcat
     5161 catgctattt catgatctat tttataaaat aaagatgtag aaaaaagtaa gcgtaataac
     5221 cgcaaaacaa atgatttaaa acatggcaca taatgaggag attaagttcg gtttacgttt
     5281 attttagtac taattgtaac gtgagactac gtatcgggaa tcgcctaatt aaagcattaa
     5341 tgcgaacctg attagattca ccgaccctcc tatcgtgtcg acctttctgt ttcttagaat
     5401 tttttggtag tctatgtact aataatgtca gcttcgtatt tatttcataa gcaatttgca
     5461 tttgcaattt gttttttact tttattttta ttgtattgtg gaatgtggac tcgtaccaac
     5521 atgaagttat ataccaccaa aaaaattaca gttagtcaaa agattcacga gtgagagcta
     5581 cttatgattg tcttttacgt atatgtctaa ttgtctattt gctcaataat ctttgtactt
     5641 tcttttgtcg ttgataaaat cacaaagttc caaaagtaat cgaatgattt gcttttaaga
     5701 aaagaagagc tcaataattc aacatatatc tgtacacagt ttaaaccaat tcgcccttcc
     5761 ccaagtgcag cgtttaataa atcgtgtgac aggatatatc ttgtggtcag gtagataaat
     5821 ttgtgtggag gtatgtttgt ttaggacggc ggtgatgatg gcttgcgcct tacgtcccga
     5881 aagcagtcca cgaagacaat tgcaatatcg gctgtcttcc attcccttcg tggcatcttg
     5941 tcaacgcgcc tcgggagctc agtcgccgac gtttcgcgag ttcggtatgc aaccccgacc
     6001 ttgcgaagaa ccgcttacgg aaccgcgctc tatgagaacc gccgggagaa gagtttcctt
     6061 gggtggggct gaagtctcgc cctttaagcg ggcgatcagg tcttctgtgg cagccgtcgc
     6121 gagatccctg acaggttgcg aaatcacggt caatggcggc gttatagcgg atgtccagtc
     6181 ggcgtcgtca aagcagataa gagagacatc atcgggaatc gaaagatcca tttcccgaag
     6241 aactccaagg agaacgaggg ctacggagct gtctgtgggg gacgtcaggt ggcacttttc
     6301 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6361 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6421 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6481 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6541 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6601 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6661 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6721 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6781 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6841 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6901 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6961 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7021 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7081 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7141 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7201 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7261 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7321 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7501 cgctaccgag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7561 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7621 cacccttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtgtg
     8101 accgggtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     8161 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     8221 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     8281 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     8341 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     8401 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     8461 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     8521 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     8581 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     8641 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     8701 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     8761 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     8821 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     8881 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     8941 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     9001 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     9061 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     9121 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     9181 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     9241 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     9301 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     9361 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     9421 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     9481 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     9541 cgggccggat ggtttgcgac cgtcacgccg attcctcggg cttgggggtt ccagtgccat
     9601 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
     9661 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
     9721 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
     9781 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
     9841 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
     9901 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
     9961 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    10021 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    10081 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    10141 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    10201 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    10261 ccagctccac caggtcggcg gtggcccata tatgtcgtaa gggcttggct gcaccggaat
    10321 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    10381 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    10441 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    10501 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    10561 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    10621 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    10681 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    10741 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    10801 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    10861 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    10921 ggtttcatgc ttgttcctct tggcgttcat tc
//
