LOCUS       pCDH-SFFV-GLuc-C        9792 bp    DNA     circular UNA 10-DEC-2018
DEFINITION  #184.
ACCESSION   urn.local...i-9unfekf
VERSION     urn.local...i-9unfekf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     gene            1798..1916
                     /standard_name="cPPT"
     gene            1928..2244
                     /standard_name="SFFV"
     gene            2257..3054
                     /standard_name="GLuc"
     gene            3070..3123
                     /standard_name="T2A"
     gene            3130..5100
                     /standard_name="CreERT2"
     gene            5107..5163
                     /standard_name="P2A mut"
     gene            5170..5880
                     /standard_name="mCherry"
     gene            5887..6477
                     /standard_name="WPRE"
ORIGIN      
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtgat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
     1981 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
     2041 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
     2101 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
     2161 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
     2221 aaaagagctc acaacccctc actcgaattc gccaccatgg ctctcccagt gactgcccta
     2281 ctgcttcccc tagcgcttct cctgcatgcc aagcccaccg agaacaacga agacttcaac
     2341 atcgtggccg tggccagcaa cttcgcgacc acggatctcg atgctgaccg cgggaagttg
     2401 cccggcaaga agctgccgct ggaggtgctc aaagagatgg aagccaatgc ccggaaagct
     2461 ggctgcacca ggggctgtct gatctgcctg tcccacatca agtgcacgcc caagatgaag
     2521 aagttcatcc caggacgctg ccacacctac gaaggcgaca aagagtccgc acagggcggc
     2581 ataggcgagg cgatcgtgga cattcctgag attcctgggt tcaaggactt ggagccaatg
     2641 gagcagttca tcgcacaggt cgatctgtgt gtggactgca caactggctg cctcaaaggg
     2701 cttgccaacg tgcagtgttc tgacctgctc aagaagtggc tgccgcaacg ctgtgcgacc
     2761 tttgccagca agatccaggg ccaggtggac aagatcaagg gggccggtgg tgacttagcg
     2821 gccgcaccca ccacgacgcc agcgccgcga ccaccaaccc cggcgcccac gatcgcgtcg
     2881 cagcccctat ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg
     2941 agggggctgg acttcgcctg tgatatctac atctgggcgc ccctggccgg gacttgtggg
     3001 gtccttctcc tgtcactggt tatcaccctt tactgcaacc acaggcggat tcaaggatcc
     3061 gcggccgctg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     3121 cctatgcata tgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat
     3181 gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt
     3241 tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag
     3301 ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat
     3361 cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg
     3421 cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg
     3481 cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc
     3541 gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat
     3601 atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt
     3661 gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt
     3721 ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta
     3781 actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac
     3841 ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca
     3901 actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat
     3961 gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga
     4021 gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat
     4081 gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc
     4141 ctgctggaag atggcgattc tgctggagac atgagagctg ccaacctttg gccaagcccg
     4201 ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc cgaccagatg
     4261 gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga tcctaccaga
     4321 cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag ggagctggtt
     4381 cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct ccatgatcag
     4441 gtccaccttc tagaatgtgc ctggctagag atcctgatga ttggtctcgt ctggcgctcc
     4501 atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag gaaccaggga
     4561 aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc atctcggttc
     4621 cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat tttgcttaat
     4681 tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa ggaccatatc
     4741 caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa ggcaggcctg
     4801 accctgcagc agcagcacca gcggctggcc cagctcctcc tcatcctctc ccacatcagg
     4861 cacatgagta acaaaggcat ggagcatctg tacagcatga agtgcaagaa cgtggtgccc
     4921 ctctatgacc tgctgctgga ggcggccgac gcccaccgcc tacatgcgcc cactagccgt
     4981 ggaggggcat ccgtggagga gacggaccaa agccacttgg ccactgcggg ctctacttca
     5041 tcgcattcct tgcaaaagta ttacatcacg ggggaggcag agggtttccc tgccacagtc
     5101 cccggggcca cgaacttctc tctgttaaag caagcaggag atgttgaaga aaacccaggg
     5161 cctatgcaca tggtgagcaa gggcgaggag gacaacatgg ccatcatcaa ggagttcatg
     5221 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     5281 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     5341 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     5401 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     5461 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     5521 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     5581 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     5641 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     5701 tacgacgccg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     5761 tacaacgtca acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     5821 cagtacgagc gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     5881 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5941 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6001 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6061 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6121 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6181 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6241 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6301 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6361 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6421 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     6481 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6541 ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac
     6601 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6661 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6721 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6781 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6841 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6901 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6961 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc
     7021 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7081 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     7141 agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7201 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7261 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7321 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7381 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7981 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8221 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8281 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8341 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8401 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8461 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8521 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8581 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8641 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8701 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8761 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8821 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8881 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8941 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9001 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9061 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9301 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     9361 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     9421 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     9481 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     9541 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     9601 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     9661 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     9721 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     9781 agtgccaagc tg
//
