LOCUS       Exported                3270 bp DNA     circular SYN 25-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 21, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    422..571
                     /label=H-NS promoter region
     CDS             572..985
                     /codon_start=1
                     /label=H-NS protein
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEES
                     AAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDENG
                     ETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     primer_bind     complement(1049..1065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1073..1089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1097..1127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1142..1163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1451..2039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2210..3070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3071..3175)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cttctggcta attttatgaa aagatattta ttggcggcac aaaataaaga acaattttga
      481 attccttaca ttcctggcta ttgcacaact gaatttaagg ctctattatt acctcaacaa
      541 accaccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa ttctgaacaa
      601 catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg aagaaatgct
      661 ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg ctgctgctga
      721 agttgaagag cgcactcgta aactgcagca atatcgcgaa atgctgatcg ctgacggtat
      781 tgacccgaac gaactgctga atagccttgc tgccgttaaa tctggcacca aagctaaacg
      841 tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta aaacctggac
      901 tggccaaggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag gtaaatccct
      961 cgacgatttc ctgatcaagc aataatcttt tgtagattgc acttgctcta gagtcgacct
     1021 gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1081 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1141 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1201 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1261 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1321 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1381 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1441 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1501 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1621 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1801 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1861 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1921 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1981 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2041 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2101 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2161 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2221 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2281 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2341 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2401 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2461 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2521 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2581 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2641 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2701 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2761 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2821 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2881 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2941 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3001 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3061 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3121 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3181 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3241 aaaataggcg tatcacgagg ccctttcgtc
//
