LOCUS       Addgene178   7124 bp    DNA   circular  SYN        7-DEC-2021
DEFINITION  Complementary copy of pFB_VSV_LOV_tK_switched.
ACCESSION   .
VERSION     .
KEYWORDS    Addgene_pCLBM1_MCS.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2021Äê12ÔÂ7ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-14165206|
COMMENT     VNTNAME|Addgene178203 pBMCL1_MCS|
COMMENT     VNTAUTHORNAME|Lei Chen|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_recomb     complement(183..348)
                     /vntifkey="86"
                     /label=Tn7L
                     /note="Tn7L"
     misc_feature    complement(377..659)
                     /vntifkey="21"
                     /label=HSVtkPolyA_pfastbacdual
                     /note="HSVtkPolyA_pfastbacdual"
     misc_feature    complement(686..1048)
                     /vntifkey="21"
                     /label=creiLOV
                     /note="creiLOV"
     misc_feature    complement(1049..1258)
                     /vntifkey="21"
                     /label=VSV-GED
                     /note="VSV-GED"
     misc_feature    complement(1259..1372)
                     /vntifkey="21"
                     /label=GP64_signal\peptide
                     /note="GP64_signal peptide"
     promoter        complement(1378..1506)
                     /vntifkey="30"
                     /label=PH\promoter
                     /note="PH promoter"
     primer_bind     1473..1490
                     /vntifkey="28"
                     /label=Primer_Bind_1
                     /note="Polyhedrin forward primer"
     misc_feature    1516..1536
                     /vntifkey="21"
                     /label=Link1
                     /note="Link1"
     promoter        1539..2122
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
                     /note="CMV_immearly_promoter"
     promoter        2062..2143
                     /vntifkey="30"
                     /label=CMV_promoter
                     /note="CMV_promoter"
     misc_feature    2147..2279
                     /vntifkey="21"
                     /label=chimeric\intron
                     /note="chimeric intron"
     misc_recomb     2287..2319
                     /vntifkey="86"
                     /label=Misc_Recomb_1
                     /note="MCS"
     CDS             2320..2337
                     /codon_start=1
                     /vntifkey="4"
                     /label=CDS_1
                     /note="Feature 23"
     misc_feature    2346..2921
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE"
     polyA_signal    2953..3193
                     /vntifkey="25"
                     /label=SV40\pA
                     /note="SV40 pA"
     misc_feature    3200..3249
                     /vntifkey="21"
                     /label=Link2
                     /note="Link2"
     promoter        3593..3620
                     /vntifkey="30"
                     /label=Pc\promoter
                     /note="Pc promoter"
     CDS             3637..4323
                     /codon_start=1
                     /vntifkey="4"
                     /label=Gm(R)
                     /note="Gm"
     misc_recomb     complement(4390..4614)
                     /vntifkey="86"
                     /label=Tn7R
                     /note="Tn7R"
     rep_origin      complement(4860..5533)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=pUC\origin
                     /note="pUC origin"
     CDS             complement(5678..6538)
                     /codon_start=1
                     /vntifkey="4"
                     /label=Amp(R)
                     /note="Amp"
     promoter        complement(6539..6637)
                     /vntifkey="30"
                     /label=bla\promoter
                     /note="bla promoter"
     rep_origin      complement(6669..7123)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=f1\origin
                     /note="f1 origin"
BASE COUNT     1749 a      1806 c      1771 g      1798 t 
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa 
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg 
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc 
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt 
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa 
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag 
      361 gctcaagcag tgatcagtgg ctatggcagg gcttgccgcc ccgacgttgg ctgcgagccc 
      421 tgggccttca cccgaacttg ggggttgggg tggggaaaag gaagaaacgc gggcgtattg 
      481 gtcccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg 
      541 tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag 
      601 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc catctcccgc 
      661 tctagtactt ctcgacaagc ttttaggcca gggctttacc ctcggtcttg gaggtgacgt 
      721 ccacctggac acccacgaac ttggacacgc gaccgtcggg agtcttgatg ggggtcacag 
      781 tcagcaggtt ccagaaaggg gtaccgtcct tgcggtagtt cagcagacgc acggagcagg 
      841 cttcaccctt cttgatagcg tcgcggatct tctgcacctc cttggggtcg gtaccttcac 
      901 cctgcaggaa gcgggcgttg tggcccagca cttcgtcggg gccgtaacca gtcatggcgt 
      961 agaagccctc ggaagcgtac accagggggc agtcgggcag agtagcgtca gccaccacga 
     1021 aggtgtgacg cagaccggca ccgccacctt tacccaggcg gttcatctcg atgtcagtat 
     1081 atatctgacg cttcttggtg tgcttcagct tgatgcacag gtggataccc acgcgcagga 
     1141 ccaggaacag accgatgatc aggccgatga tgaagaagaa agaagcgatg ctggacttcc 
     1201 agctgctgaa ccagccttcg accagttcga tagggttctt gctcagaccg gtgtcaccag 
     1261 cgaaggcgga gtgagcagca gcagccagca gcacgtacag cacgatagcg ctcaccatct 
     1321 tgctagtgtg ctccttgttg aaaccctggt ggctctggtt caccagcagc atagatccgc 
     1381 gcccgatggt gggacggtat gaataatccg gaatatttat aggttttttt attacaaaac 
     1441 tgttacgaaa acagtaaaat acttatttat ttgcgagatg gttatcattt taattatctc 
     1501 catgatctat taatacttaa ttaacaacac catttgttgg cattgattat tgactagtta 
     1561 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 
     1621 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 
     1681 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 
     1741 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc 
     1801 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 
     1861 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 
     1921 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc 
     1981 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt 
     2041 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg 
     2101 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcaaggtaa gtatcaaggt 
     2161 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt 
     2221 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg 
     2281 tgtaaggaat tcggatcccg ggcggccgct agcctcgagc accaccacca ccaccactga 
     2341 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 
     2401 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 
     2461 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 
     2521 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 
     2581 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 
     2641 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 
     2701 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg 
     2761 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 
     2821 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 
     2881 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtcga 
     2941 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 
     3001 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 
     3061 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     3121 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     3181 tcatgtctgg atctgatcag taacaacacc atttaaatgg agtggttaca aatggagtgg 
     3241 ttaattaagt tccggagtat acgtagccaa ccactagaac tatagctaga gtcctgggcg 
     3301 aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc 
     3361 accaccggca agcgccgcga cggccgaggt cttccgatct cctgaagcca gggcagatcc 
     3421 gtgcacagca ccttgccgta gaagaacagc aaggccgcca atgcctgacg atgcgtggag 
     3481 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc 
     3541 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gttgacataa 
     3601 gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga 
     3661 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac 
     3721 tgtttttttg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt 
     3781 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca 
     3841 aagttaggtg gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc 
     3901 aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac 
     3961 tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc 
     4021 gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc 
     4081 aagtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac 
     4141 cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt 
     4201 ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat 
     4261 acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc 
     4321 taacaattcg ttcaagccga gatcggcttc ccggccgcgg agttgttcgg taaattgtca 
     4381 caacgccgcg aatatagtct ttaccatgcc cttggccacg cccctcttta atacgacggg 
     4441 caatttgcac ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt 
     4501 tttcacagca taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta 
     4561 ttgtcatagt ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct 
     4621 tacgcagggc atccatttat tactcaaccg taaccgattt tgccaggtta cgcggctggt 
     4681 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 
     4741 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     4801 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     4861 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     4921 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     4981 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     5041 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     5101 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     5161 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     5221 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     5281 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     5341 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     5401 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     5461 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     5521 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     5581 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     5641 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     5701 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     5761 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     5821 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     5881 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     5941 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     6001 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     6061 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     6121 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     6181 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     6241 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     6301 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     6361 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     6421 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     6481 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     6541 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     6601 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     6661 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat 
     6721 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata 
     6781 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taagaacgtg 
     6841 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca 
     6901 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa 
     6961 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg 
     7021 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta 
     7081 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtc 
//