LOCUS       pUC-TalO8-NotI          4358 bp    DNA     circular UNA 01-APR-2015
DEFINITION  Vector.
ACCESSION   urn.local...k-e0j57tb
VERSION     urn.local...k-e0j57tb
KEYWORDS    .
SOURCE      S. cerevisiae
  ORGANISM  S. cerevisiae
            .
FEATURES             Location/Qualifiers
     primer_bind     359..375
                     /Sequence="GTTTTCCCAGTCACGAC"
                     /Hairpin_Tm="None"
                     /Tm="52.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.9"
                     /created_by="primer3"
                     /label="M13F(-40)"
     primer_bind     378..395
                     /Sequence="TGTAAAACGACGGCCAGT"
                     /Hairpin_Tm="None"
                     /Tm="56.8"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="M13F(-21)"
     primer_bind     437..449
                     /Sequence="GGGTACCGAGCTC"
                     /Hairpin_Tm="40.9"
                     /Tm="46.4"
                     /Self_Dimer_Tm="5.7"
                     /%GC="69.2"
                     /created_by="primer3"
                     /modified_by="User"
                     /label=941
     misc_feature    451..456
                     /Original_Bases="AATT"
                     /label="AATT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     458..474
                     /Sequence="GGTCGAAAAAAGAAAAG"
                     /Hairpin_Tm="None"
                     /Tm="45.9"
                     /Self_Dimer_Tm="None"
                     /%GC="35.3"
                     /created_by="primer3"
                     /label=941
     ORF             555..1229
                     /created_by="User"
                     /label="TRP1"
     primer_bind     complement(577..596)
                     /Sequence="CACCAATGGACCAGAACTAC"
                     /Hairpin_Tm="None"
                     /Tm="55.8"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label=933
     primer_bind     1002..1021
                     /Sequence="GGGACAGGTGAACTTTTGGA"
                     /Hairpin_Tm="None"
                     /Tm="57.6"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label=935
     primer_bind     complement(1141..1160)
                     /Sequence="TCCGCTTACATCAACACCAA"
                     /Hairpin_Tm="None"
                     /Tm="57.4"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /created_by="primer3"
                     /label=936
     primer_bind     1161..1180
                     /Sequence="GGTGTGGAGACAAATGGTGT"
                     /Hairpin_Tm="46.1"
                     /Tm="58.0"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label=932
     primer_bind     complement(1258..1277)
                     /Sequence="CAGGCAAGTGCACAAACAAT"
                     /Hairpin_Tm="44.4"
                     /Tm="57.8"
                     /Self_Dimer_Tm="11.3"
                     /%GC="45.0"
                     /created_by="primer3"
                     /label=937
     rep_origin      1280..1335
                     /created_by="User"
                     /label="ARS1"
     primer_bind     1473..1489
                     /Sequence="CAATGACCCGATTCTTG"
                     /Hairpin_Tm="None"
                     /Tm="49.9"
                     /Self_Dimer_Tm="None"
                     /%GC="47.1"
                     /created_by="primer3"
                     /label="GibPr"
     primer_bind     complement(1494..1510)
                     /Sequence="GCAAGACCGAGAAAAGG"
                     /Hairpin_Tm="None"
                     /Tm="52.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.9"
                     /created_by="primer3"
                     /label="Gib3'"
     primer_bind     complement(1542..1562)
                     /Sequence="GGAGAGAGGTATGTACATGTG"
                     /Hairpin_Tm="None"
                     /Tm="54.9"
                     /Self_Dimer_Tm="18.8"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label=961
     primer_bind     1812..1832
                     /Sequence="CAATGCGAGATCCGTTTAACC"
                     /Hairpin_Tm="41.2"
                     /Tm="57.7"
                     /Self_Dimer_Tm="1.3"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label=959
     primer_bind     1813..1831
                     /Sequence="AATGCGAGATCCGTTTAAC"
                     /Hairpin_Tm="41.2"
                     /Tm="53.5"
                     /Self_Dimer_Tm="0.7"
                     /%GC="42.1"
                     /created_by="primer3"
                     /label="pDTL11"
     primer_bind     1861..1878
                     /Sequence="GTCCACTGTGTGCCGAAC"
                     /Hairpin_Tm="31.3"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="61.1"
                     /created_by="primer3"
                     /modified_by="User"
                     /label=945
     misc_feature    1879..2097
                     /Original_Bases="ATGC"
                     /label="ATGC"
                     /note="Geneious type: Editing History Replacement"
     DNA_probe_bind  complement(1883..1900)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(1910..1927)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(1937..1954)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(1964..1981)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(1991..2008)
                     /Sequence="TGTTATCTGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="48.5"
                     /Self_Dimer_Tm="None"
                     /%GC="33.3"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(2017..2035)
                     /Sequence="TGTTATCCGCTCACAATTG"
                     /Hairpin_Tm="None"
                     /Tm="53.4"
                     /Self_Dimer_Tm="3.2"
                     /%GC="42.1"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(2045..2062)
                     /Sequence="TGTTATCCGCTCATAATT"
                     /Hairpin_Tm="None"
                     /Tm="48.1"
                     /Self_Dimer_Tm="None"
                     /%GC="33.3"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(2072..2089)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     primer_bind     complement(2105..2119)
                     /Sequence="CACATGTTAAAATAG"
                     /Hairpin_Tm="None"
                     /Tm="35.7"
                     /Self_Dimer_Tm="None"
                     /%GC="26.7"
                     /modified_by="User"
                     /label=958
     misc_feature    2120^2121
                     /Original_Bases="AATTC"
                     /label="AATTC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    2122..2127
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(2137..2153)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Hairpin_Tm="None"
                     /Tm="49.1"
                     /Self_Dimer_Tm="None"
                     /%GC="47.1"
                     /created_by="primer3"
                     /label="M13R Reverse"
     DNA_probe_bind  complement(2162..2179)
                     /Sequence="AATTGTGAGCGGATAACA"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="LacO"
     primer_bind     complement(2223..2243)
                     /Sequence="GTTAGCTCACTCATTAGGCAC"
                     /Hairpin_Tm="None"
                     /Tm="56.4"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label=960
     DNA_probe_bind  complement(3298..4158)
                     /Sequence="ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGC
                     ATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
                     GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCC
                     TTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCT
                     ATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
                     CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGG
                     ATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGC
                     GGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
                     AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCA
                     TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAA
                     ACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
                     GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTA
                     TTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGG
                     GCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
                     ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGT
                     AA"
                     /created_by="User"
                     /label="AmpR"
     primer_bind     3550..3569
                     /Sequence="TTGCCGGGAAGCTAGAGTAA"
                     /Hairpin_Tm="None"
                     /Tm="58.4"
                     /Self_Dimer_Tm="8.3"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label=928
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg cggccgcggt cgaaaaaaga aaaggagagg
      481 gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa
      541 aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag
      601 tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc
      661 tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa
      721 tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg
      781 tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata
      841 tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag
      901 ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga
      961 aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg
     1021 attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta
     1081 tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta
     1141 ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag
     1201 caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt
     1261 gtttgtgcac ttgcctgcag gccttttgaa aagcaagcat aaaagatcta aacataaaat
     1321 ctgtaaaata acaagatgta aagataatgc taaatcattt ggctttttga ttgattgtac
     1381 aggaaaatat acatcgcagg gggttgactt ttaccatttc accgcaatgg aatcaaactt
     1441 gttgaagaga atgttcacag gcgcatacgc tacaatgacc cgattcttgc tagccttttc
     1501 tcggtcttgc aaacaaccgc cggcagctta gtatataaat acacatgtac atacctctct
     1561 ccgtatcctc gtaatcattt tcttgtattt atcgtctttt cgctgtaaaa actttatcac
     1621 acttatctca aatacactta ttaaccgctt ttactattat cttctacgct gacagtaata
     1681 tcaaacagtg acacatatta aacacagtgg tttctttgca taaacaccat cagcctcaag
     1741 tcgtcaagta aagatttcgt gttcatgcag atagataaca atctatatgt tgataattag
     1801 cgttgcctca tcaatgcgag atccgtttaa ccggacccta gtgcacttac cccacgttcg
     1861 gtccactgtg tgccgaacta gcaattgtga gcggataaca attactagca attgtgagcg
     1921 gataacaatt actagcaatt gtgagcggat aacaattact agcaattgtg agcggataac
     1981 aattactagc aattgtgagc agataacaat tactagcaat tgtgagcgga taacaattac
     2041 tagcaattat gagcggataa caattactag caattgtgag cggataacaa tactagttcc
     2101 ttcactattt taacatgtgg cggccgcgta atcatggtca tagctgtttc ctgtgtgaaa
     2161 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     2221 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2281 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2341 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2401 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2461 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2521 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2581 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2641 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2701 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2881 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2941 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3001 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3061 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3601 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3721 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3901 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4261 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4321 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
