LOCUS       Glc3prUTR-CFP-Ad        5729 bp    DNA     circular UNA 23-OCT-2015
DEFINITION  Vector.
ACCESSION   urn.local...g-e0j57t9
VERSION     urn.local...g-e0j57t9
KEYWORDS    .
SOURCE      S. cerevisiae
  ORGANISM  S. cerevisiae
            .
FEATURES             Location/Qualifiers
     primer_bind     359..375
                     /Sequence="GTTTTCCCAGTCACGAC"
                     /Hairpin_Tm="None"
                     /Tm="52.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.9"
                     /created_by="primer3"
                     /label="M13F(-40)"
     primer_bind     378..395
                     /Sequence="TGTAAAACGACGGCCAGT"
                     /Hairpin_Tm="None"
                     /Tm="56.8"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="M13F(-21)"
     primer_bind     437..449
                     /Sequence="GGGTACCGAGCTC"
                     /Hairpin_Tm="40.9"
                     /Tm="46.4"
                     /Self_Dimer_Tm="5.7"
                     /%GC="69.2"
                     /created_by="primer3"
                     /modified_by="User"
                     /label=941
     primer_bind     458..474
                     /Sequence="GGTCGAAAAAAGAAAAG"
                     /Hairpin_Tm="None"
                     /Tm="45.9"
                     /Self_Dimer_Tm="None"
                     /%GC="35.3"
                     /created_by="primer3"
                     /label=941
     ORF             555..1229
                     /created_by="User"
                     /label="TRP1"
     primer_bind     complement(577..596)
                     /Sequence="CACCAATGGACCAGAACTAC"
                     /Hairpin_Tm="None"
                     /Tm="55.8"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label=933
     primer_bind     1002..1021
                     /Sequence="GGGACAGGTGAACTTTTGGA"
                     /Hairpin_Tm="None"
                     /Tm="57.6"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label=935
     primer_bind     complement(1141..1160)
                     /Sequence="TCCGCTTACATCAACACCAA"
                     /Hairpin_Tm="None"
                     /Tm="57.4"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /created_by="primer3"
                     /label=936
     primer_bind     1161..1180
                     /Sequence="GGTGTGGAGACAAATGGTGT"
                     /Hairpin_Tm="46.1"
                     /Tm="58.0"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label=932
     primer_bind     complement(1258..1277)
                     /Sequence="CAGGCAAGTGCACAAACAAT"
                     /Hairpin_Tm="44.4"
                     /Tm="57.8"
                     /Self_Dimer_Tm="11.3"
                     /%GC="45.0"
                     /created_by="primer3"
                     /label=937
     rep_origin      1280..1335
                     /created_by="User"
                     /label="ARS1"
     primer_bind     1473..1489
                     /Sequence="CAATGACCCGATTCTTG"
                     /Hairpin_Tm="None"
                     /Tm="49.9"
                     /Self_Dimer_Tm="None"
                     /%GC="47.1"
                     /created_by="primer3"
                     /label="GibPr"
     promoter        1490..1885
                     /created_by="User"
                     /label="prGlc3"
     DNA_probe_bind  complement(1513..1517)
                     /Sequence="CCCCT"
                     /created_by="User"
                     /label="STRE"
     DNA_probe_bind  1605..1609
                     /Sequence="CCCCT"
                     /created_by="User"
                     /label="STRE"
     DNA_probe_bind  complement(1674..1678)
                     /Sequence="CCCCT"
                     /created_by="User"
                     /label="STRE"
     ORF             1886..2602
                     /created_by="User"
                     /label="CFP"
     3'UTR           2603..2864
                     /created_by="User"
                     /label="Adh1_3'"
     origin          2604^2605
                     /label="origin"
     primer_bind     complement(2865..2881)
                     /Sequence="GCAAGACCGAGAAAAGG"
                     /Hairpin_Tm="None"
                     /Tm="52.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.9"
                     /created_by="primer3"
                     /label="Gib3'"
     primer_bind     complement(2913..2933)
                     /Sequence="GGAGAGAGGTATGTACATGTG"
                     /Hairpin_Tm="None"
                     /Tm="54.9"
                     /Self_Dimer_Tm="18.8"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label=961
     primer_bind     3183..3203
                     /Sequence="CAATGCGAGATCCGTTTAACC"
                     /Hairpin_Tm="41.2"
                     /Tm="57.7"
                     /Self_Dimer_Tm="1.3"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label=959
     primer_bind     3184..3202
                     /Sequence="AATGCGAGATCCGTTTAAC"
                     /Hairpin_Tm="41.2"
                     /Tm="53.5"
                     /Self_Dimer_Tm="0.7"
                     /%GC="42.1"
                     /created_by="primer3"
                     /label="pDTL11"
     primer_bind     3232..3249
                     /Sequence="GTCCACTGTGTGCCGAAC"
                     /Hairpin_Tm="31.3"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="61.1"
                     /created_by="primer3"
                     /modified_by="User"
                     /label=945
     DNA_probe_bind  complement(3254..3271)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(3281..3298)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(3308..3325)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(3335..3352)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(3362..3379)
                     /Sequence="TGTTATCTGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="48.5"
                     /Self_Dimer_Tm="None"
                     /%GC="33.3"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(3388..3406)
                     /Sequence="TGTTATCCGCTCACAATTG"
                     /Hairpin_Tm="None"
                     /Tm="53.4"
                     /Self_Dimer_Tm="3.2"
                     /%GC="42.1"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(3416..3433)
                     /Sequence="TGTTATCCGCTCATAATT"
                     /Hairpin_Tm="None"
                     /Tm="48.1"
                     /Self_Dimer_Tm="None"
                     /%GC="33.3"
                     /created_by="primer3"
                     /label="lacO"
     DNA_probe_bind  complement(3443..3460)
                     /Sequence="TGTTATCCGCTCACAATT"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="lacO"
     primer_bind     complement(3476..3490)
                     /Sequence="CACATGTTAAAATAG"
                     /Hairpin_Tm="None"
                     /Tm="35.7"
                     /Self_Dimer_Tm="None"
                     /%GC="26.7"
                     /modified_by="User"
                     /label=958
     misc_feature    3491^3492
                     /Original_Bases="AATTC"
                     /label="AATTC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3493..3498
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(3508..3524)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Hairpin_Tm="None"
                     /Tm="49.1"
                     /Self_Dimer_Tm="None"
                     /%GC="47.1"
                     /created_by="primer3"
                     /label="M13R Reverse"
     DNA_probe_bind  complement(3533..3550)
                     /Sequence="AATTGTGAGCGGATAACA"
                     /Hairpin_Tm="None"
                     /Tm="51.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.9"
                     /created_by="primer3"
                     /label="LacO"
     primer_bind     complement(3594..3614)
                     /Sequence="GTTAGCTCACTCATTAGGCAC"
                     /Hairpin_Tm="None"
                     /Tm="56.4"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label=960
     DNA_probe_bind  complement(4669..5529)
                     /Sequence="ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGC
                     ATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
                     GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCC
                     TTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCT
                     ATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
                     CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGG
                     ATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGC
                     GGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
                     AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCA
                     TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAA
                     ACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
                     GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTA
                     TTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGG
                     GCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
                     ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGT
                     AA"
                     /created_by="User"
                     /label="AmpR"
     primer_bind     4921..4940
                     /Sequence="TTGCCGGGAAGCTAGAGTAA"
                     /Hairpin_Tm="None"
                     /Tm="58.4"
                     /Self_Dimer_Tm="8.3"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label=928
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg cggccgcggt cgaaaaaaga aaaggagagg
      481 gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa
      541 aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag
      601 tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc
      661 tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa
      721 tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg
      781 tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata
      841 tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag
      901 ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga
      961 aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg
     1021 attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta
     1081 tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta
     1141 ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag
     1201 caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt
     1261 gtttgtgcac ttgcctgcag gccttttgaa aagcaagcat aaaagatcta aacataaaat
     1321 ctgtaaaata acaagatgta aagataatgc taaatcattt ggctttttga ttgattgtac
     1381 aggaaaatat acatcgcagg gggttgactt ttaccatttc accgcaatgg aatcaaactt
     1441 gttgaagaga atgttcacag gcgcatacgc tacaatgacc cgattcttgg tgaattctgt
     1501 agtctgtcat ccaggggaag ggcagggcac tcatcaacaa tgtgatagtc agaaagcaat
     1561 aatgttttgt attctgatga gctttcacta tctcttccgc aatacccctt tttgcctata
     1621 ttacggcatc atctttcccc gcgttctcgt gggggccaaa taagctttgt ccaaggggct
     1681 gttgcaacgg taaaggtagt cgagccaagt gacaccagca aacaaggaaa caaggaagca
     1741 aggaaggctt tccattttcc aataacgaca tgagtctatc tatggtgtat aaaagtggca
     1801 ggcttttcct acattttttt tccctgataa cttcctgtta ctatttaaga acaccaaacc
     1861 aagtataaag aaccgtcaag aataaatgtc taagggtgaa gaattgttca ctggtgttgt
     1921 tccaatcttg gttgaattgg acggtgacgt taacggtcac aagttctctg tttctggtga
     1981 aggtgaaggt gacgctactt acggtaagtt gactttgaag ttcatctgta ctactggtaa
     2041 gttgccagtt ccatggccaa ctttggttac tactttgact tggggtgttc aatgtttcgc
     2101 tagataccca gaccacatga agcaacacga cttcttcaag tctgctatgc cagaaggtta
     2161 cgttcaagaa agaactatct tcttcaagga cgacggtaac tacaagacta gagctgaagt
     2221 taagttcgaa ggtgacactt tggttaacag aatcgaattg aagggtatcg acttcaagga
     2281 agacggtaac atcttgggtc acaagttgga atacaactac atctctgaca acgtttacat
     2341 cactgctgac aagcaaaaga acggtatcaa ggctaacttc aagatcagac acaacatcga
     2401 agacggtggt gttcaattgg ctgaccacta ccaacaaaac actccaatcg gtgacggtcc
     2461 agttttgttg ccagacaacc actacttgtc tactcaatct aagttgtcta aggacccaaa
     2521 cgaaaagaga gaccacatgg ttttgttgga attcgttact gctgctggta tcactttggg
     2581 tatggacgaa ttgtacaagt aaggcgcgcc acttctaaat aagcgaattt cttatgattt
     2641 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
     2701 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
     2761 ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg cagatccgct
     2821 agggataaca gggtaatata gatctgttta gcttgcctcg tccccctttt ctcggtcttg
     2881 caaacaaccg ccggcagctt agtatataaa tacacatgta catacctctc tccgtatcct
     2941 cgtaatcatt ttcttgtatt tatcgtcttt tcgctgtaaa aactttatca cacttatctc
     3001 aaatacactt attaaccgct tttactatta tcttctacgc tgacagtaat atcaaacagt
     3061 gacacatatt aaacacagtg gtttctttgc ataaacacca tcagcctcaa gtcgtcaagt
     3121 aaagatttcg tgttcatgca gatagataac aatctatatg ttgataatta gcgttgcctc
     3181 atcaatgcga gatccgttta accggaccct agtgcactta ccccacgttc ggtccactgt
     3241 gtgccgaact agcaattgtg agcggataac aattactagc aattgtgagc ggataacaat
     3301 tactagcaat tgtgagcgga taacaattac tagcaattgt gagcggataa caattactag
     3361 caattgtgag cagataacaa ttactagcaa ttgtgagcgg ataacaatta ctagcaatta
     3421 tgagcggata acaattacta gcaattgtga gcggataaca atactagttc cttcactatt
     3481 ttaacatgtg gcggccgcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3721 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4201 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4261 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4321 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     4381 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4441 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4501 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4561 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4621 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4681 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4741 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4801 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4861 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4921 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4981 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5041 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5101 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5161 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5221 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5281 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5341 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5401 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5461 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5521 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5581 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5641 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     5701 aaataggcgt atcacgaggc cctttcgtc
//
