LOCUS       Exported                4583 bp DNA     circular SYN 25-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Baylor-NRI
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 26, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    17..234
                     /label=ASP17JANA1
     polyA_signal    21..69
                     /note="synthetic polyadenylation signal"
     misc_feature    83..174
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     protein_bind    187..205
                     /label=HRE - Hypoxia
                     /bound_moiety="hypoxia-inducible factor HIF-1"
                     /note="hypoxia response element (4 copies)"
     protein_bind    212..230
                     /label=HRE - Hypoxia
                     /bound_moiety="hypoxia-inducible factor HIF-1"
                     /note="hypoxia response element (4 copies)"
     misc_feature    235..392
                     /label=ASP17JANA2
     protein_bind    239..257
                     /label=HRE - Hypoxia
                     /bound_moiety="hypoxia-inducible factor HIF-1"
                     /note="hypoxia response element (4 copies)"
     protein_bind    264..282
                     /label=HRE - Hypoxia
                     /bound_moiety="hypoxia-inducible factor HIF-1"
                     /note="hypoxia response element (4 copies)"
     primer_bind     291..313
                     /label=SEQ MINIP FWD
     promoter        329..360
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(370..392)
                     /label=SEQ MiniP REV
     CDS             394..2040
                     /codon_start=1
                     /label=RedFirefly
                     /translation="MENMENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFT
                     NAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGV
                     APTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGY
                     QCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS
                     HARDPIYGNQVSPGTAVLTVVPLHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQD
                     YKCTYVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQ
                     GYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPML
                     MKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELES
                     VLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGG
                     VRFVDEVPKGLTGKIDGRAIREILKKPVAKM"
     polyA_signal    2045..2269
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2288..2313
                     /label=ASP14NOV50
     rep_origin      complement(2375..3057)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(3070..3095)
                     /label=ASP14NOV51
     primer_bind     3092..3116
                     /label=ASP14JUL17
     promoter        3096..3194
                     /label=cat Promoter
     primer_bind     complement(3179..3198)
                     /label=ASP14OCT52
     primer_bind     3195..3217
                     /label=ASP14OCT53
     CDS             3199..4011
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3993..4016)
                     /label=ASP14OCT54
     primer_bind     4012..4038
                     /label=ASP14OCT55
     terminator      4016..4123
     primer_bind     complement(4104..4133)
                     /label=ASP14JUL20
     primer_bind     complement(4104..4127)
                     /label=ASP14JUL20
     primer_bind     4124..4147
                     /label=ASP15JAN01
     rep_origin      4128..4583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4500..4518)
                     /label=ASP14NOV53
     primer_bind     complement(join(4552..4583,1..6))
                     /label=ASP15JUL28
ORIGIN
        1 taacgaattc gtctcaggag aataaaatat ctttattttc attacatctg tgtgttggtt
       61 ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca
      121 aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctcgctgt
      181 caggaggtga ctacgtgctg cctagagatc tgtgactacg tgctgcctag gcggccgcgt
      241 gactacgtgc tgcctagtct agagtgacta cgtgctgcct agtccctcga ggatatcaaa
      301 tctggcctcg gcggccaagc ttagacacta gagggtatat aatggaagct cgacttccag
      361 cttggcaatc cggtactgtt ggtaaagcca ccaatggaga acatggagaa cgacgagaac
      421 attgtggtgg gacccaagcc gttctacccg atcgaggagg gttccgctgg cacccagctg
      481 cgcaagtaca tggagcgcta cgctaagctg ggtgctatcg ccttcaccaa cgccgtgacc
      541 ggtgtggatt actcctacgc cgagtacctg gagaagtcct gctgcctcgg aaaggccctg
      601 cagaactacg gtctggtcgt ggatggccgc attgccctct gctcggagaa ctgcgaggag
      661 ttcttcattc ccgtgatcgc tggtctgttc atcggagtcg gcgttgcccc aaccaacgag
      721 atctacaccc tgcgcgagct ggtccacagc ctgggtatca gcaagccgac catcgttttc
      781 tcgagcaaga agggcctcga caaggttatc accgtgcaaa aaaccgtcac caccatcaag
      841 accatcgtta tcctggattc caaggttgac taccgcggtt accagtgcct cgacaccttc
      901 atcaagcgta acaccccacc cggcttccaa gcctcctcct tcaaaaccgt ggaggttgat
      961 cgcaaggagc aggtggccct gatcatgaac tcctccggct ccaccggtct gccaaagggc
     1021 gtgcagctga cccacgagaa caccgtcacc cgcttctcgc atgcccgtga tcccatctac
     1081 ggcaaccagg tgtccccagg caccgccgtc ctgaccgtcg tgcccctcca ccacggcttc
     1141 ggcatgttca ccactctcgg ctacttgatc tgcggcttcc gtgtggtgat gctgactaag
     1201 ttcgacgagg agactttcct caagaccctg caagactaca agtgcactta cgtgatcctg
     1261 gtgccgaccc tgttcgccat cctgaacaag agcgagctgt tgaacaaata cgacctgtcg
     1321 aacctggtgg agatcgcttc cggcggcgcc ccactgtcga aggaagttgg cgaggccgtt
     1381 gctcgccgct tcaacctgcc cggcgtccgt cagggttacg gcctgaccga gactacctcg
     1441 gctatcatca tcacccccga gggcgacgat aagcccggcg cttccggcaa ggttgttcca
     1501 ctcttcaaag ccaaggtgat cgacctggac accaagaagt ccctgggtcc caaccgtcgc
     1561 ggagaagtgt gcgtcaaggg ccccatgctg atgaagggct acgtcaacaa cccagaggcc
     1621 accaaggagt tgatcgacga ggagggctgg ctgcacactg gtgacatcgg ttactacgac
     1681 gaagagaagc acttcttcat cgttgaccgc ctgaagtcct tgatcaagta caagggctac
     1741 caggttcccc ccgccgagct cgagagcgtc ctgctgcagc acccctccat tttcgatgcc
     1801 ggagtggccg gtgtccccga tcccgtggct ggcgagctgc caggtgccgt cgtcgtgctg
     1861 gagtccggca agaacatgac cgagaaggaa gtgatggatt acgtggcctc gcaagtgtcc
     1921 aacgccaagc gcctgcgcgg cggcgtgcgc ttcgtggatg aggtgcccaa gggcctcacc
     1981 ggaaagatcg acggtcgcgc tatccgcgag atcctcaaga agcccgtggc caagatgtga
     2041 gcttctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2101 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2161 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2221 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc gctgtcatga
     2281 gacgaattct gacacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2341 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2401 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2461 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2521 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2581 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2641 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2701 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2761 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2821 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2881 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2941 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3001 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3061 tatcaaaaag gatcttcacc tagatccttt tgtgatgatc ggcacgtaag aggttccaac
     3121 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     3181 ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctc aaggccgcga
     3241 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     3301 caatcaggtg cgacaatcta ccgattgtat gggaagcccg atgcgccaga gttgtttctg
     3361 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     3421 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     3481 tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct
     3541 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     3601 cctgtttgta attgtccttt taacagcgat cgcgtatttc gactcgctca ggcgcaatca
     3661 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3721 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3781 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3841 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3901 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3961 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctgatttttt
     4021 ttaaggcagt tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa
     4081 gcggatgaat ggcagaaatt cgatgataag ctgtcaaaca tgatgttacg cgccctgtag
     4141 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4201 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4261 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4321 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccag cgccctgata
     4381 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4441 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4501 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4561 caaaatatta acgcttacaa ttt
//
