LOCUS       Exported                3878 bp DNA     circular SYN 25-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Baylor-NRI
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 26, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     polyA_signal    25..73
                     /note="synthetic polyadenylation signal"
     misc_feature    87..178
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_binding    191..198
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    203..210
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     protein_bind    214..217
                     /label=RE
                     /bound_moiety="NF-kappa-B"
                     /note="NF-kappa-B response element (5 copies)"
     misc_feature    214..217
                     /label=ASP17JANA2
     misc_binding    221..228
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    235..242
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    250..257
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    265..272
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    279..286
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     primer_bind     301..323
                     /label=SEQ MINIP FWD
     promoter        339..370
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(380..402)
                     /label=SEQ MiniP REV
     primer_bind     399..428
                     /label=ASP16JUN01
     primer_bind     403..426
                     /label=ASP16JUL18
     CDS             404..1339
                     /codon_start=1
                     /label=GreenRenilla_HS
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGSALAFHYAYEHQDRIKAIVHMESVVDVIESWMGWPDIEEEL
                     ALIKSEEGEKMVLENNFFVETLLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             404..1339
                     /codon_start=1
                     /label=GreenRenilla_HS Split
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGSALAFHYAYEHQDRIKAIVHMESVVDVIESWMGWPDIEEEL
                     ALIKSEEGEKMVLENNFFVETLLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ"
     primer_bind     404..428
                     /label=ASP16JUN01
     primer_bind     complement(1067..1091)
                     /label=ASP16JUL19
     primer_bind     1091..1112
                     /label=ASP16JUL20
     primer_bind     complement(1321..1344)
                     /label=ASP16JUN02
     polyA_signal    1344..1568
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1670..2352)
                     /direction=LEFT
                     /label=ColE1 origin
     promoter        2391..2489
                     /label=cat Promoter
     CDS             2494..3306
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      3311..3418
     rep_origin      3423..3878
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 taacgatatc gtctcatggc ggagaataaa atatctttat tttcattaca tctgtgtgtt
       61 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
      121 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctcg
      181 ctgtcaggag gtctagactg ccgtctagac ttaacagtac gtctagactc ggccgtctag
      241 acttagtacg tctagactcg gtatgtctag actgacccgt ctagacggat cctccctcga
      301 ggatatcaaa tctggcctcg gcggccaagc ttagacacta gagggtatat aatggaagct
      361 cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccaatggctt ccaaagtcta
      421 cgacccagaa caaaggaaga ggatgattac tggaccgcag tggtgggcaa ggtgcaagca
      481 gatgaatgta ctcgatagtt ttatcaatta ttacgactcc gaaaaacacg cggaaaatgc
      541 tgtcatattc ctgcacggga acgccactag cagctacttg tggcggcacg tagtccccca
      601 catagagcct gttgcccgat gtattattcc cgatctcatt ggaatgggca aatcaggcaa
      661 atctgggaac ggttcttacc gcctgctcga tcactataaa tatttgaccg cttggtttga
      721 gctgcttaat ctccctaaaa aaataatatt cgttggacac gactggggct cagctctggc
      781 gttccactac gcatacgagc atcaagaccg aataaaagca atagtacata tggagtcagt
      841 tgttgacgtc atagagtcat ggatgggttg gccagatatt gaagaggaat tggccctcat
      901 caagagtgag gagggggaaa aaatggtcct ggaaaataat tttttcgtgg aaaccttgct
      961 cccgagtaaa ataatgcgaa aacttgaacc cgaagagttt gctgcttatt tggagccttt
     1021 caaggaaaag ggggaagtaa gacgaccaac cctttcttgg ccgagggaga tacctcttgt
     1081 taagggtgga aagcccgacg tagtccagat agtccgaaat tacaacgcgt acctgagagc
     1141 tagtgatgac ctccctaagt tgtttatcga gtcagaccct gggttcttca gcaacgcaat
     1201 tgtagaagga gcgaaaaaat ttccaaatac agaatttgtg aaggttaagg ggctccactt
     1261 tttgcaagag gacgcccccg atgagatggg aaaatacatt aaatctttcg tagagcgcgt
     1321 ccttaagaat gaacagtgag cttctgtgcc ttctagttgc cagccatctg ttgtttgccc
     1381 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     1441 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     1501 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     1561 ctctatggcg cttgagacga tatctgacac ggttatccac agaatcaggg gataacgcag
     1621 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1681 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1741 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1801 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1861 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1921 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1981 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2041 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2101 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2161 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2221 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2281 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2341 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgtga tgatcggcac
     2401 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     2461 ttatcgagat tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct
     2521 tgctcaaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     2581 cgcgataatg tcgggcaatc aggtgcgaca atctaccgat tgtatgggaa gcccgatgcg
     2641 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2701 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2761 actcctgatg atgcatggtt actcaccact gcgatcccag ggaaaacagc attccaggta
     2821 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2881 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgactc
     2941 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3001 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     3061 ccggattcag tagtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3121 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3181 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3241 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3301 tttttctgat tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg
     3361 aataagtgat aataagcgga tgaatggcag aaattcgatg ataagctgtc aaacatgatg
     3421 ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3481 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3541 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3601 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3661 gccagcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3721 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3781 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3841 taacgcgaat tttaacaaaa tattaacgct tacaattt
//
