LOCUS       Exported                6055 bp ds-DNA     circular SYN 01-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 25, 2021 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2517..2681
                     /label=ITR
                     /note="ITR"
     misc_feature    2698..2703
                     /label=Killed BglII
                     /note="BglII"
     misc_feature    2704..3165
                     /label=synapsin promoter
                     /note="synapsin promoter"
     misc_feature    2787..2805
                     /label=synapsin forward
                     /note="synapsin forward"
     misc_feature    3126..3146
                     /label=hsyn seq for
                     /note="hsyn seq for"
     misc_feature    3194..3199
                     /label=Kozak
     sig_peptide     3200..3262
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
     regulatory      3200..3203
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3263..3274
                     /codon_start=1
                     /label=MCS
                     /translation="RSGS"
     CDS             3275..4021
                     /codon_start=1
                     /label=GltI 5-253
                     /translation="AAGSTLDKIAKNGVIVVGHRDSSVPFSYYDSQHKVVGYSQEYSNA
                     IVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTYDFECGSTTNNVERQKQAAFSDTIF
                     VVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAKDHGDS
                     FRTLESGRAVAFMMDDSLLAGERAKAKKPDDWEIVGKPQSQEAYGCMLRKDDPQFKKLM
                     DDTIAQVQTSGEAEKWFDKWFKNPILV"
     CDS             4022..4297
                     /codon_start=1
                     /label=cpSFGFP
                     /translation="NHTVYITADKQKNGIKANFKIHHNVEDGSVQLADHYQQNTPIGDG
                     PVLLPDNHYLSYQSVLSKDPNEKRDHMVLLEFVTATGNSLGMDELYK"
     CDS             4298..4315
                     /codon_start=1
                     /label=Linker
                     /translation="GGTGGS"
     CDS             4316..4753
                     /codon_start=1
                     /label=cpSFGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKLEGDTLVNRIELKGIDFKEDGNILGHKLEYNFN"
     CDS             4754..4831
                     /codon_start=1
                     /label=GltI 254-279
                     /translation="NPLNMNFELSDEMKALFKEPNDKALK"
     CDS             4832..4843
                     /codon_start=1
                     /label=MCS
                     /translation="LQVD"
     CDS             4844..4873
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4874..4990
                     /codon_start=1
                     /label=B16 GPI_COBL9 Anchor
                     /translation="SDNDYGKDGLAGASGGRRNGAITVLSFITFYVAAFMVLL"
     misc_feature    5006..5594
                     /label=WPRE
                     /note="WPRE"
     misc_feature    5077..5096
                     /label=WPRE rev
                     /note="WPRE rev"
     misc_feature    5142..5162
                     /label=Mao 115r
                     /note="Mao 115r"
     primer_bind     5644..5667
                     /label=f.ITR for 3.01.15
                     /note="f.ITR for 3.01.15"
     primer_bind     5784..5804
                     /label=f.ITR for 4.01.16
                     /note="f.ITR for 4.01.16"
     misc_feature    5888..6052
                     /label=ITR
                     /note="ITR"
     misc_feature    6055
                     /label=33_1304 seq
                     /note="33_1304 seq"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2041 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2101 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2161 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2221 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2281 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2341 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2401 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     2461 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctgcgcg
     2521 ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc
     2581 cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt
     2641 ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc tctaggaaga
     2701 tccagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2761 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2821 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2881 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2941 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     3001 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     3061 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     3121 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgagtccaa ttcaagctgc
     3181 tagcaaggat cccgccacca tggagacaga cacactcctg ctatgggtac tgctgctctg
     3241 ggttccaggt tccactggtg acagatctgg gtccgccgcc ggaagtactc ttgataagat
     3301 cgctaaaaat ggtgtaattg ttgtcggcca ccgagactct tctgtgccat tcagctatta
     3361 tgattctcaa cacaaagtgg tgggctattc acaagagtat tctaacgcca ttgttgaagc
     3421 agttaagaag aagttgaata aaccagacct tcaagtaaaa ctcattccta taacatccca
     3481 aaaccgcata cctctcttgc aaaacggcac ttacgatttc gagtgtggga gcactactaa
     3541 taatgtcgaa cgccagaaac aagccgcatt ctccgacacc atcttcgtgg tgggcactcg
     3601 actgttgaca aagaaaggcg gtgatattaa ggatttcgca aatctgaagg ataaggcagt
     3661 cgtggtcacc agcgggacca cctcagaagt actccttaat aaactcaatg aagaacaaaa
     3721 gatgaatatg cgaatcatct ctgctaagga ccacggtgac tcttttcgca cattggaaag
     3781 tgggcgcgcc gtagcattta tgatggacga ctctctgctc gctggcgagc gcgccaaagc
     3841 caaaaaacca gacgactggg aaattgtagg taaacctcaa agccaagagg catatggctg
     3901 catgttgaga aaagatgacc cccaattcaa gaaactgatg gacgacacta tagctcaggt
     3961 ccagacttca ggggaagctg agaagtggtt tgataaatgg ttcaagaacc caatcttggt
     4021 aaatcatacc gtttatatta ccgctgacaa acaaaagaat gggataaagg caaatttcaa
     4081 gattcaccat aacgtagagg atggaagcgt gcagttggca gaccattatc aacagaatac
     4141 ccctattggc gacggacccg tgctcctccc tgacaaccac tacttgtcct atcaaagtgt
     4201 tctctcaaag gaccccaatg agaagcgaga ccacatggta cttctcgaat tcgttaccgc
     4261 caccgggaat tcacttggca tggatgaatt gtataagggg ggtactggcg gcagcatgtc
     4321 caagggtgag gagcttttta ccggagtagt accaattctc gttgaacttg acggagacgt
     4381 caacgggcac aagtttagcg tacgaggtga gggggagggt gatgcaacca acggtaaact
     4441 tactctcaaa ttcatatgca ctaccggaaa gctgccagta ccttggccaa cactggtcac
     4501 taccctctcc tatggcgttc aatgttttgc tcgataccct gaccacatga aacagcatga
     4561 cttctttaaa tcagccatgc ctgaagggta tgtccaggag cggactatat catttaaaga
     4621 cgatggaacc tataagactc gggctgaagt gaagctcgag ggtgacacat tggttaatcg
     4681 gatcgagctt aagggcatag attttaaaga agatggtaat attcttggcc acaaattgga
     4741 gtacaatttt aataatcctt tgaacatgaa ctttgagctc tccgatgaaa tgaaggcact
     4801 ctttaaagag ccaaacgaca aagctctgaa actgcaggtc gacgaacaaa aactcatctc
     4861 agaagaggat ctgtcagata acgactacgg taaagatggg ttggctgggg cttcaggagg
     4921 cagacgaaat ggcgctataa ctgtgctcag tttcataacg ttctacgtgg cagcatttat
     4981 ggtattgttg tagaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     5041 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5101 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5161 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5221 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5281 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5341 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5401 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5461 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5521 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5581 ggccgcctcc ccgcatcgat accgtcgacc tcgacccggg cggccgcttc gagcagacat
     5641 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     5701 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     5761 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     5821 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc ttcctagagc
     5881 atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga
     5941 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     6001 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcag
//
