LOCUS       Exported                6760 bp ds-DNA     circular SYN 01-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 25, 2021 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(23..187)
                     /label=ITR
                     /note="ITR"
     misc_feature    complement(194..236)
                     /label=seq
                     /note="seq"
     misc_feature    complement(251..270)
                     /label=pzac2.1 5088f
                     /note="pzac2.1 5088f"
     primer_bind     complement(351..374)
                     /label=f.ITR for 2.01.14
                     /note="f.ITR for 2.01.14"
     primer_bind     complement(452..472)
                     /label=f.ITR for 1.01.13
                     /note="f.ITR for 1.01.13"
     primer_bind     2471..2489
                     /label=r.ITR rev 3.01.19
                     /note="r.ITR rev 3.01.19"
     primer_bind     2513..2536
                     /label=r.ITR rev 4.01.20
                     /note="r.ITR rev 4.01.20"
     misc_feature    complement(2707..2871)
                     /label=ITR
                     /note="ITR"
     promoter        2898..3832
                     /label=CAG
                     /note="CAG "
     primer_bind     2975..2994
                     /label=f.CAG promoter seq.33.1743
                     /note="f.CAG promoter seq.33.1743"
     primer_bind     complement(3043..3058)
                     /label=r.CAG-flex ITR seq.33.1306 CAG rev
                     /note="r.CAG-flex ITR seq.33.1306 CAG rev"
     primer_bind     3049..3066
                     /label=f.CAG promoter.33.1744
                     /note="f.CAG promoter.33.1744"
     primer_bind     3483..3499
                     /label=f.CAG promoter seq.33.1747
                     /note="f.CAG promoter seq.33.1747"
     primer_bind     3495..3516
                     /label=r.CAG promoter seq.33.1745
                     /note="r.CAG promoter seq.33.1745"
     primer_bind     complement(3581..3603)
                     /label=r.CAG promoter seq.33.1746
                     /note="r.CAG promoter seq.33.1746"
     primer_bind     3703..3725
                     /label=f.CAG for.01.40
                     /note="f.CAG for.01.40"
     primer_bind     complement(3795..3818)
                     /label=r.CAG promoter seq.33.1748
                     /note="r.CAG promoter seq.33.1748"
     misc_feature    3833
                     /label=pCAG_GCaMP3
     misc_feature    3840..3845
                     /label=Kozak
     sig_peptide     3846..3908
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
     regulatory      3846..3849
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3909..3920
                     /codon_start=1
                     /label=MCS
                     /translation="RSGS"
     CDS             3921..4667
                     /codon_start=1
                     /label=GltI 5-253
                     /translation="AAGSTLDKIAKNGVIVVGHRDSSVPFSYYDSQHKVVGYSQEYSNA
                     IVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTYDFECGSTTNNVERQKQAAFSDTIF
                     VVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAKDHGDS
                     FRTLESGRAVAFMMDDSLLAGERAKAKKPDDWEIVGKPQSQEAYGCMLRKDDPQFKKLM
                     DDTIAQVQTSGEAEKWFDKWFKNPILV"
     CDS             4668..4943
                     /codon_start=1
                     /label=cpSFGFP
                     /translation="NHTVYITADKQKNGIKANFKIHHNVEDGSVQLADHYQQNTPIGDG
                     PVLLPDNHYLSYQSVLSKDPNEKRDHMVLLEFVTATGNSLGMDELYK"
     CDS             4944..4961
                     /codon_start=1
                     /label=Linker
                     /translation="GGTGGS"
     CDS             4962..5399
                     /codon_start=1
                     /label=cpSFGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKLEGDTLVNRIELKGIDFKEDGNILGHKLEYNFN"
     CDS             5400..5477
                     /codon_start=1
                     /label=GltI 254-279
                     /translation="NPLNMNFELSDEMKALFKEPNDKALK"
     CDS             5478..5489
                     /codon_start=1
                     /label=MCS
                     /translation="LQVD"
     CDS             5490..5519
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5520..5582
                     /codon_start=1
                     /label=PDGFR-beta hinge
                     /translation="NAVGQDTQEVIVVPHSLPFKV"
     CDS             5583..5645
                     /codon_start=1
                     /label=PDGFR-beta TM domain
                     /translation="VVISAILALVVLTIISLIILI"
     CDS             5646..5669
                     /codon_start=1
                     /label=PDGFR-beta Export fragment
                     /translation="MLWQKKPR"
     CDS             5670..5870
                     /codon_start=1
                     /label=Stargazin 203-319 targeting S->D
                     /translation="DRHKQLRATARATDYLQADAITRIPDYRYRYQRRDRDDDRDTEPD
                     HDRDADPVGVKGFNTLPDTEID"
     CDS             5871..5891
                     /codon_start=1
                     /label=PDZ recognition motif
                     /translation="NPQTTSV"
     misc_feature    5907..6495
                     /label=WPRE
                     /note="WPRE"
     primer_bind     complement(5978..5997)
                     /label=r.wpre rev.01.3
                     /note="r.wpre rev.01.3"
     primer_bind     complement(6308..6328)
                     /label=wpre rev pcr 32.63
                     /note="wpre rev pcr 32.63"
     primer_bind     6332..6351
                     /label=f.WPRE seq.01.80
                     /note="f.WPRE seq.01.80"
     primer_bind     6539..6562
                     /label=f.ITR for 3.01.15
                     /note="f.ITR for 3.01.15"
ORIGIN
        1 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca
       61 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
      121 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
      181 gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      241 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      841 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1201 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1261 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1321 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1381 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1441 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1501 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1561 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1621 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1681 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1741 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1801 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1861 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1921 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1981 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2041 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2161 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     2221 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2281 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     2341 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2401 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2461 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2521 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     2581 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     2641 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2701 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
     2761 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
     2821 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
     2881 tctaggaaga tcgtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2941 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3001 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3061 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3121 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     3181 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     3241 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     3301 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     3361 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     3421 gcgggagtcg ctgcgacgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     3481 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3541 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3601 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggctgtc
     3661 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     3721 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     3781 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttggatcccg
     3841 ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt ccaggttcca
     3901 ctggtgacag atctgggtcc gccgccggaa gtactcttga taagatcgct aaaaatggtg
     3961 taattgttgt cggccaccga gactcttctg tgccattcag ctattatgat tctcaacaca
     4021 aagtggtggg ctattcacaa gagtattcta acgccattgt tgaagcagtt aagaagaagt
     4081 tgaataaacc agaccttcaa gtaaaactca ttcctataac atcccaaaac cgcatacctc
     4141 tcttgcaaaa cggcacttac gatttcgagt gtgggagcac tactaataat gtcgaacgcc
     4201 agaaacaagc cgcattctcc gacaccatct tcgtggtggg cactcgactg ttgacaaaga
     4261 aaggcggtga tattaaggat ttcgcaaatc tgaaggataa ggcagtcgtg gtcaccagcg
     4321 ggaccacctc agaagtactc cttaataaac tcaatgaaga acaaaagatg aatatgcgaa
     4381 tcatctctgc taaggaccac ggtgactctt ttcgcacatt ggaaagtggg cgcgccgtag
     4441 catttatgat ggacgactct ctgctcgctg gcgagcgcgc caaagccaaa aaaccagacg
     4501 actgggaaat tgtaggtaaa cctcaaagcc aagaggcata tggctgcatg ttgagaaaag
     4561 atgaccccca attcaagaaa ctgatggacg acactatagc tcaggtccag acttcagggg
     4621 aagctgagaa gtggtttgat aaatggttca agaacccaat cttggtaaat cataccgttt
     4681 atattaccgc tgacaaacaa aagaatggga taaaggcaaa tttcaagatt caccataacg
     4741 tagaggatgg aagcgtgcag ttggcagacc attatcaaca gaatacccct attggcgacg
     4801 gacccgtgct cctccctgac aaccactact tgtcctatca aagtgttctc tcaaaggacc
     4861 ccaatgagaa gcgagaccac atggtacttc tcgaattcgt taccgccacc gggaattcac
     4921 ttggcatgga tgaattgtat aaggggggta ctggcggcag catgtccaag ggtgaggagc
     4981 tttttaccgg agtagtacca attctcgttg aacttgacgg agacgtcaac gggcacaagt
     5041 ttagcgtacg aggtgagggg gagggtgatg caaccaacgg taaacttact ctcaaattca
     5101 tatgcactac cggaaagctg ccagtacctt ggccaacact ggtcactacc ctctcctatg
     5161 gcgttcaatg ttttgctcga taccctgacc acatgaaaca gcatgacttc tttaaatcag
     5221 ccatgcctga agggtatgtc caggagcgga ctatatcatt taaagacgat ggaacctata
     5281 agactcgggc tgaagtgaag ctcgagggtg acacattggt taatcggatc gagcttaagg
     5341 gcatagattt taaagaagat ggtaatattc ttggccacaa attggagtac aattttaata
     5401 atcctttgaa catgaacttt gagctctccg atgaaatgaa ggcactcttt aaagagccaa
     5461 acgacaaagc tctgaaactg caggtcgacg aacaaaaact catctcagaa gaggatctga
     5521 atgctgtggg ccaggacacg caggaggtca tcgtggtgcc acactccttg ccctttaagg
     5581 tggtggtgat ctcagccatc ctggccctgg tggtgctcac catcatctcc cttatcatcc
     5641 tcatcatgct ttggcagaag aagccacgtg atagacacaa gcagttgcga gctacagcac
     5701 gcgccaccga ttatctgcaa gctgatgcaa taactagaat ccctgattat agatacagat
     5761 atcaacgaag ggatcgcgat gatgatcgag atacagagcc agatcatgat cgggatgctg
     5821 atcccgtcgg ggtgaaaggt ttcaacactc ttccagatac agagatagat aatccccaga
     5881 cgacttcagt gtagaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     5941 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6001 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6061 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6121 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6181 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6241 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6301 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6361 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6421 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6481 gggccgcctc cccgcatcga taccgtcgac ccgggcggcc gcttcgagca gacatgataa
     6541 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     6601 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     6661 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     6721 aaagcaagta aaacctctac aaatgtggta aaatcgataa
//
