LOCUS       #252_pCax_CIP4-GFP      7081 bp ds-DNA     linear       22-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        4..383
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        4..1670
                     /label="CAG Promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        386..661
                     /label="Chicken Beta Actin Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        662..1670
                     /label="Chimeric Intron"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Primer          1668..1686
                     /label="Dent_Seq_10"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1732..1755
                     /label="Exon1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1732..3366
                     /label="hCip4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1732..4098
                     /label="Translation 1732-4098"
     misc_feature    1746..2502
                     /label="F-Bar"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Primer          complement(1750..1769)
                     /label="Dent_Seq_43"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1756..1871
                     /label="Exon 2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1872..1928
                     /label="Exon 3"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1929..2076
                     /label="Exon 4"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2077..2139
                     /label="Exon 5"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2140..2244
                     /label="Exon 6"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2245..2373
                     /label="Exon 7"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Primer          2292..2311
                     /label="Dent_Seq_7"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2374..2520
                     /label="Exon 8"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2503..2747
                     /label=" "
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2521..2715
                     /label="Exon 9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2716..2825
                     /label="Exon 11 (10 MIA)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2748..2988
                     /label="HR1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2794..3066
                     /label="BD Antibody Site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2826..2958
                     /label="Exon 12"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2959..3098
                     /label="Exon 13"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3099..3220
                     /label="Exon 14"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3195..3357
                     /label="SH3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3221..3366
                     /label="Exon 15"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Primer          3249..3268
                     /label="Dent_Seq_41"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3382..4098
                     /label="EGFP from pcDNA3-EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Primer          complement(3427..3448)
                     /label="Dent_Seq_25"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Primer          4035..4056
                     /label="Dent_Seq_42"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             4099..4101
                     /label="Translation 4099-4101"
     Primer          complement(4224..4243)
                     /label="Dent_Seq_11"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4580..4600)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Primer          complement(4584..4600)
                     /label="Dent_Seq_14"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_binding    complement(4606..4628)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(4632..4662)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(5313..5995)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(6093..6752)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
       61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
      121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
      181 GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
      241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
      301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
      361 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC
      421 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA
      481 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG
      541 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT
      601 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC
      661 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC
      721 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC
      781 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG
      841 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG
      901 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG
      961 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC
     1021 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG
     1081 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC
     1141 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC
     1201 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG
     1261 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT
     1321 GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG
     1381 GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC
     1441 TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT
     1501 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG
     1561 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG
     1621 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA
     1681 ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGA ATTCAGGTAC CATGGATTGG
     1741 GGCACTGAGC TGTGGGATCA GTTCGAGGTG CTCGAGCGCC ACACGCAGTG GGGGCTGGAC
     1801 CTGTTGGACA GATATGTAAA GTTCGTGAAA GAACGCACCG AAGTGGAACA GGCTTACGCC
     1861 AAACAACTGC GGAGCCTGGT GAAAAAATAT CTGCCCAAGA GACCTGCCAA GGATGATCCT
     1921 GAGTCCAAAT TCAGCCAGCA ACAGTCCTTC GTACAGATTC TCCAGGAGGT GAATGACTTT
     1981 GCAGGCCAGC GGGAGCTGGT GGCTGAGAAC CTCAGTGTCC GTGTATGTCT TGAGCTGACC
     2041 AAGTACTCAC AAGAGATGAA ACAGGAGAGG AAGATGCACT TCCAAGAAGG GCGGCGGGCC
     2101 CAGCAGCAGC TGGAAAATGG CTTTAAACAG CTGGAGAATA GTAAGCGTAA ATTTGAGCGG
     2161 GACTGCCGGG AGGCAGAGAA GGCAGCCCAG ACTGCTGAAC GGCTAGACCA GGATATCAAC
     2221 GCCACCAAGG CTGATGTGGA GAAGGCCAAG CAGCAAGCCC ACCTTCGGAG TCACATGGCC
     2281 GAAGAAAGCA AAAACGAATA TGCGGCTCAA CTGCAGCGCT TCAACCGAGA CCAAGCCCAC
     2341 TTCTATTTTT CACAGATGCC CCAGATATTC GATAAGCTCC AAGACATGGA TGAACGCAGG
     2401 GCCACCCGCC TGGGTGCCGG GTATGGGCTC CTGTCGGAGG CCGAGCTGGA GGTGGTGCCC
     2461 ATAATAGCCA AGTGCTTGGA GGGCATGAAG GTGGCTGCAA ATGCTGTGGA TCCCAAGAAC
     2521 GACTCCCACG TCCTTATAGA GCTGCACAAG TCAGGTTTTG CCCGCCCGGG CGACGTGGAA
     2581 TTCGAGGACT TCAGCCAGCC CATGAACCGT GCACCCTCCG ACAGCAGTCT GGGCACCCCC
     2641 TCGGATGGAC GGCCTGAACT CCGAGGCCCG GGTCGCAGCC GCACCAAGCG CTGGCCTTTT
     2701 GGCAAGAAGA ACAAGACAGT GGTGACCGAG GATTTTAGCC ACTTGCCCCC AGAGCAGCAG
     2761 CGAAAACGGC TTCAACAGCA GTTGGAAGAA CGCAGTCGTG AACTTCAGAA GGAGGTTGAC
     2821 CAGAGGGAAG CCCTAAAGAA AATGAAGGAT GTCTATGAGA AGACACCTCA GATGGGGGAC
     2881 CCCGCCAGCT TGGAGCCCCA GATCGCTGAA ACCCTGAGCA ACATTGAACG GCTGAAATTG
     2941 GAAGTGCAGA AGTATGAGGC GTGGCTGGCA GAAGCTGAAA GTCGAGTCCT TAGCAACCGG
     3001 GGAGACAGCC TGAGCCGGCA CGCCCGGCCT CCCGACCCCC CCGCTAGCGC CCCGCCAGAC
     3061 AGCAGCAGCA ACAGCGCATC ACAGGACACC AAGGAGAGCT CTGAAGAGCC TCCCTCAGAA
     3121 GAGAGCCAGG ACACCCCCAT TTACACGGAG TTTGATGAGG ATTTCGAGGA GGAACCCACA
     3181 TCCCCCATAG GTCACTGTGT GGCCATCTAC CACTTTGAAG GGTCCAGCGA GGGCACTATC
     3241 TCTATGGCCG AGGGTGAAGA CCTCAGTCTT ATGGAAGAAG ACAAAGGGGA CGGCTGGACC
     3301 CGGGTCAGGC GGAAAGAGGG AGGCGAGGGC TACGTGCCCA CCTCCTACCT CCGAGTCACG
     3361 CTCAATTGCG GCCGCCTCGA GATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG
     3421 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG
     3481 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG
     3541 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC
     3601 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC
     3661 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG
     3721 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG
     3781 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC
     3841 ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG
     3901 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
     3961 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC
     4021 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
     4081 ATGGACGAGC TGTACAAGta aAGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT
     4141 GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT
     4201 GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTAAA
     4261 ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT GCCATATGCT GGCTGCCATG
     4321 AACAAAGGTG GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT GTCCATTCCT
     4381 TATTCCATAG AAAAGCCTTG ACTTGAGGTT AGATTTTTTT TATATTTTGT TTTGTGTTAT
     4441 TTTTTTCTTT AACATCCCTA AAATTTTCCT TACATGTTTT ACTAGCCAGA TTTTTCCTCC
     4501 TCTCCTGACT ACTCCCAGTC ATAGCTGTCC CTCTTCTCTT ATGAAGATCC CTCGACCTGC
     4561 AGCCCAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
     4621 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
     4681 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
     4741 TCGTGCCAGC GGATCCGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC
     4801 CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT
     4861 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG
     4921 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTAAC TTGTTTATTG CAGCTTATAA
     4981 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA
     5041 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT
     5101 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC
     5161 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA
     5221 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
     5281 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
     5341 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
     5401 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
     5461 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
     5521 TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
     5581 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     5641 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
     5701 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
     5761 TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
     5821 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
     5881 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT
     5941 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     6001 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     6061 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     6121 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     6181 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC
     6241 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     6301 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     6361 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     6421 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     6481 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     6541 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     6601 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     6661 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     6721 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     6781 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     6841 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     6901 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     6961 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     7021 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT
     7081 G
//