LOCUS       #273_pCax_FBP17_GFP     7297 bp ds-DNA     circular     22-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        4..383
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        4..1670
                     /label="CAG Promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        386..661
                     /label="Chicken Beta Actin Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        662..1670
                     /label="Chimeric Intron"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Primer          1668..1686
                     /label="Dent_Seq_10"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1721..2692
                     /label="Seq Verified 3/5/17 Clone 2 and 4"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1732..3582
                     /label="Human FBP17"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             1732..4314
                     /label="Translation 1732-4314"
     misc_feature    1747..2497
                     /label="FBAR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Primer          2354..2373
                     /label="Dent_Seq_16"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2677..2679
                     /label="q"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2683..2685
                     /label="q"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2689..2691
                     /label="q"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2707..2712
                     /label="qq"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2716..2718
                     /label="q"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2716..2913
                     /label="Missing in short form"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2838..2838
                     /label="Actually a C, silent mutation"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2902..2916
                     /label="Missing in some clones"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2931..2931
                     /label="Actually a C, Glu->Asp"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2932..3199
                     /label="Seq Verified 3/11/17"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2953..3181
                     /label="HR1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3200..3200
                     /label="Sequenced as A on fwd"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3201..3619
                     /label="Seq Verified 3/11/17"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3391..3555
                     /label="SH3"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3429..4406
                     /label="Seq Verified 3/5/17 Clone 2 Only"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3598..4314
                     /label="EGFP from pcDNA3-EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Primer          complement(3643..3664)
                     /label="Dent_Seq_25"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Primer          4251..4272
                     /label="Dent_Seq_42"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Primer          complement(4440..4459)
                     /label="Dent_Seq_11"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4796..4816)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Primer          complement(4800..4816)
                     /label="Dent_Seq_14"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_binding    complement(4822..4844)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(4848..4878)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(5529..6211)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(6309..6968)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
       61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
      121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
      181 GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
      241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
      301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
      361 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC
      421 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA
      481 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG
      541 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT
      601 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC
      661 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC
      721 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC
      781 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG
      841 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG
      901 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG
      961 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC
     1021 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG
     1081 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC
     1141 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC
     1201 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG
     1261 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT
     1321 GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG
     1381 GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC
     1441 TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT
     1501 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG
     1561 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG
     1621 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA
     1681 ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGA ATTCAGGTAC CATGAGCTGG
     1741 GGCACCGAGC TCTGGGATCA GTTTGACAAC TTAGAAAAAC ACACACAGTG GGGAATTGAT
     1801 ATTCTTGAGA AATATATCAA GTTTGTGAAA GAAAGGACAG AGATTGAACT CAGCTATGCA
     1861 AAGCAACTCA GGAATCTTTC AAAGAAGTAC CAACCTAAAA AGAACTCGAA GGAGGAAGAA
     1921 GAATACAAGT ATACGTCATG TAAAGCTTTC ATTTCCAACC TGAACGAAAT GAATGATTAC
     1981 GCAGGGCAGC ATGAAGTTAT CTCCGAGAAC ATGGCATCAC AGATCATTGT GGACTTGGCA
     2041 CGCTATGTTC AGGAACTGAA ACAGGAGAGG AAATCAAACT TTCACGATGG CCGTAAAGCA
     2101 CAGCAGCACA TCGAGACTTG CTGGAAGCAG CTTGAATCTA GTAAAAGGCG ATTTGAACGC
     2161 GATTGCAAAG AGGCGGACAG GGCGCAGCAG TACTTTGAGA AAATGGACGC TGACATCAAT
     2221 GTCACAAAAG CGGATGTTGA AAAGGCCCGA CAACAAGCTC AAATACGTCA CCAAATGGCA
     2281 GAGGACAGCA AAGCAGATTA CTCATCCATT CTCCAGAAAT TCAACCATGA GCAGCATGAA
     2341 TATTACCATA CTCACATCCC CAACATCTTC CAGAAAATAC AAGAGATGGA GGAAAGGAGG
     2401 ATTGTGAGAA TGGGAGAGTC CATGAAGACA TATGCAGAGG TTGATCGGCA GGTGATCCCA
     2461 ATCATTGGGA AGTGCCTGGA TGGAATAGTA AAAGCAGCCG AATCAATTGA TCAGAAAAAT
     2521 GATTCACAGC TGGTAATAGA AGCTTATAAA TCAGGGTTTG AGCCTCCTGG AGACATTGAA
     2581 TTTGAGGATT ACACTCAGCC AATGAAGCGC ACTGTGTCAG ATAACAGCCT TTCAAATTCC
     2641 AGAGGAGAAG GCAAACCAGA CCTCAAATTT GGTGGCAAAT CCAAAGGAAA GTTATGGCCG
     2701 TTCATCAAAA AAAATAAGCT TATGTCCCTT TTAACATCCC CCCATCAGCC TCCCCCTCCC
     2761 CCTCCTGCCT CTGCCTCACC CTCTGCTGTT CCCAACGGCC CCCAGTCTCC CAAGCAGCAA
     2821 AAGGAACCCC TCTCCCATCG CTTCAACGAG TTCATGACCT CCAAACCCAA AATCCACTGC
     2881 TTCAGGAGCC TAAAGCGTGG GCTTTCTCTC AAGCTGGGTG CAACACCGGA GGATTTCAGC
     2941 AACCTCCCAC CTGAACAAAG AAGGAAAAAG CTGCAGCAGA AAGTCGATGA GTTAAATAAA
     3001 GAAATTCAGA AGGAGATGGA TCAAAGAGAT GCCATAACAA AAATGAAAGA TGTCTACCTA
     3061 AAGAATCCTC AGATGGGAGA CCCAGCCAGT TTGGATCACA AATTAGCAGA AGTCAGCCAA
     3121 AATATAGAGA AACTGCGAGT AGAGACCCAG AAATTTGAGG CCTGGCTGGC TGAGGTTGAA
     3181 GGCCGGCTCC CAGCACGCAG CGAGCAGGCG CGCCGGCAGA GCGGACTGTA CGACAGCCAG
     3241 AACCCACCCA CAGTCAACAA CTGCGCCCAG GACCGTGAGA GCCCAGATGG CAGTTACACA
     3301 GAGGAGCAGA GTCAGGAGAG TGAGATGAAG GTGCTGGCCA CGGATTTTGA CGACGAGTTT
     3361 GATGATGAGG AGCCCCTCCC TGCCATAGGG ACGTGCAAAG CTCTCTACAC ATTTGAAGGT
     3421 CAGAATGAAG GAACGATTTC CGTAGTTGAA GGAGAAACAT TGTATGTCAT AGAGGAAGAC
     3481 AAAGGCGATG GCTGGACCCG CATTCGGAGA AATGAAGATG AAGAGGGTTA TGTCCCCACT
     3541 TCATATGTCG AAGTCTGTTT GGACAAAAAT GCCAAAGATT CCTGCGGCCG CCTCGAGATG
     3601 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
     3661 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
     3721 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
     3781 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG
     3841 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
     3901 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
     3961 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
     4021 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
     4081 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
     4141 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
     4201 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
     4261 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGA
     4321 TCTTTTTCCC TCTGCCAAAA ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC
     4381 TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC
     4441 ACTCGGAAGG ACATATGGGA GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA
     4501 GAGTTTGGCA ACATATGCCA TATGCTGGCT GCCATGAACA AAGGTGGCTA TAAAGAGGTC
     4561 ATCAGTATAT GAAACAGCCC CCTGCTGTCC ATTCCTTATT CCATAGAAAA GCCTTGACTT
     4621 GAGGTTAGAT TTTTTTTATA TTTTGTTTTG TGTTATTTTT TTCTTTAACA TCCCTAAAAT
     4681 TTTCCTTACA TGTTTTACTA GCCAGATTTT TCCTCCTCTC CTGACTACTC CCAGTCATAG
     4741 CTGTCCCTCT TCTCTTATGA AGATCCCTCG ACCTGCAGCC CAAGCTTGGC GTAATCATGG
     4801 TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC
     4861 GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG
     4921 TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCGGAT CCGCATCTCA
     4981 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA
     5041 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG
     5101 CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT
     5161 TTTGCAAAAA GCTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT
     5221 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
     5281 CATCAATGTA TCTTATCATG TCTGGATCCG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
     5341 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
     5401 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
     5461 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     5521 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     5581 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     5641 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     5701 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT
     5761 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     5821 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     5881 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     5941 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT
     6001 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     6061 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     6121 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     6181 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     6241 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     6301 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     6361 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     6421 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     6481 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     6541 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     6601 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     6661 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     6721 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     6781 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     6841 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     6901 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     6961 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     7021 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     7081 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     7141 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     7201 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     7261 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTG
//