LOCUS       #350_pCAX_FBP17_mScarle 7280 bp ds-DNA     circular     22-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        4..383
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        386..661
                     /label="Chicken Beta Actin Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1556..1561
                     /label="SacII"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     Primer          1673..1691
                     /label="Dent_Seq_10"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1731..1736
                     /label="kpn1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1737..3587
                     /label="Human FBP17"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             1737..4295
                     /label="Translation 1737-4295"
     misc_feature    1752..2502
                     /label="FBAR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Primer          2359..2378
                     /label="Dent_Seq_16"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2682..2684
                     /label="q"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2688..2690
                     /label="q"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2694..2696
                     /label="q"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2712..2717
                     /label="qq"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2721..2723
                     /label="q"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2721..2918
                     /label="Missing in short form"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2843..2843
                     /label="Actually a C, silent mutation"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2907..2921
                     /label="Missing in some clones"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2936..2936
                     /label="Actually a C, Glu->Asp"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2937..3204
                     /label="Seq Verified 3/11/17"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2958..3186
                     /label="HR1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3205..3205
                     /label="Sequenced as A on fwd"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3396..3560
                     /label="SH3"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3588..3593
                     /label="Not1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3600..3602
                     /label="ATG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             3603..4295
                     /label="mScarlet"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer_bind     complement(3733..3751)
                     /label="mCherry-R"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Primer          3782..3801
                     /label="Dent_Seq_23"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Primer          complement(3782..3801)
                     /label="Dent_Seq_22"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(3783..3797)
                     /label="DsRed1-N"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Primer          4001..4020
                     /label="Dent_Seq_19"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     4001..4020
                     /label="mCherry-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4115..4115
                     /label="mut"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     Primer          4136..4155
                     /label="Dent_Seq_24"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     4198..4218
                     /label="DsRed1-C"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4306..4737
                     /label="rb_glob_pA terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Primer          complement(4421..4440)
                     /label="Dent_Seq_11"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4779..4799)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Primer          complement(4783..4799)
                     /label="Dent_Seq_14"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_binding    complement(4805..4827)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(4831..4861)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(5512..6194)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(6292..6951)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
       61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
      121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
      181 GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
      241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
      301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
      361 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC
      421 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA
      481 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG
      541 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT
      601 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC
      661 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC
      721 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC
      781 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG
      841 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG
      901 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG
      961 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC
     1021 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG
     1081 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC
     1141 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTaG CGGGGCGTGG
     1201 CGCGGGGCTC GCCGTGCCGG GCGGGGGGTG GCGGCAGGTG GGGGTGCCGG GCGGGGCGGG
     1261 GCCGCCTCGG GCCGGGGAGG GCTCGGGGGA GGGGCGCGGC GGcCCCCGGA GCGCCGGCGG
     1321 CTGTCGAGGC GCGGCGAGCC GCAGCCATTG CCTTTTATGG TAATCGTGCG AGAGGGCGCA
     1381 GGGACTTCCT TTGTCCCAAA TCTGtGCGGA GCCGAAATCT GGGAGGCGCC GCCGCACCCC
     1441 CTCTAGCGGG CGCGGGgCGA AGCGGTGCGG CGCCGGCAGG AAGGAAATGG GCGGGGAGGG
     1501 CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC CAGCCTCGGG GCTGTCCGCG
     1561 GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC
     1621 CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT
     1681 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA GGTACCATGA
     1741 GCTGGGGCAC CGAGCTCTGG GATCAGTTTG ACAACTTAGA AAAACACACA CAGTGGGGAA
     1801 TTGATATTCT TGAGAAATAT ATCAAGTTTG TGAAAGAAAG GACAGAGATT GAACTCAGCT
     1861 ATGCAAAGCA ACTCAGGAAT CTTTCAAAGA AGTACCAACC TAAAAAGAAC TCGAAGGAGG
     1921 AAGAAGAATA CAAGTATACG TCATGTAAAG CTTTCATTTC CAACCTGAAC GAAATGAATG
     1981 ATTACGCAGG GCAGCATGAA GTTATCTCCG AGAACATGGC ATCACAGATC ATTGTGGACT
     2041 TGGCACGCTA TGTTCAGGAA CTGAAACAGG AGAGGAAATC AAACTTTCAC GATGGCCGTA
     2101 AAGCACAGCA GCACATCGAG ACTTGCTGGA AGCAGCTTGA ATCTAGTAAA AGGCGATTTG
     2161 AACGCGATTG CAAAGAGGCG GACAGGGCGC AGCAGTACTT TGAGAAAATG GACGCTGACA
     2221 TCAATGTCAC AAAAGCGGAT GTTGAAAAGG CCCGACAACA AGCTCAAATA CGTCACCAAA
     2281 TGGCAGAGGA CAGCAAAGCA GATTACTCAT CCATTCTCCA GAAATTCAAC CATGAGCAGC
     2341 ATGAATATTA CCATACTCAC ATCCCCAACA TCTTCCAGAA AATACAAGAG ATGGAGGAAA
     2401 GGAGGATTGT GAGAATGGGA GAGTCCATGA AGACATATGC AGAGGTTGAT CGGCAGGTGA
     2461 TCCCAATCAT TGGGAAGTGC CTGGATGGAA TAGTAAAAGC AGCCGAATCA ATTGATCAGA
     2521 AAAATGATTC ACAGCTGGTA ATAGAAGCTT ATAAATCAGG GTTTGAGCCT CCTGGAGACA
     2581 TTGAATTTGA GGATTACACT CAGCCAATGA AGCGCACTGT GTCAGATAAC AGCCTTTCAA
     2641 ATTCCAGAGG AGAAGGCAAA CCAGACCTCA AATTTGGTGG CAAATCCAAA GGAAAGTTAT
     2701 GGCCGTTCAT CAAAAAAAAT AAGCTTATGT CCCTTTTAAC ATCCCCCCAT CAGCCTCCCC
     2761 CTCCCCCTCC TGCCTCTGCC TCACCCTCTG CTGTTCCCAA CGGCCCCCAG TCTCCCAAGC
     2821 AGCAAAAGGA ACCCCTCTCC CATCGCTTCA ACGAGTTCAT GACCTCCAAA CCCAAAATCC
     2881 ACTGCTTCAG GAGCCTAAAG CGTGGGCTTT CTCTCAAGCT GGGTGCAACA CCGGAGGATT
     2941 TCAGCAACCT CCCACCTGAA CAAAGAAGGA AAAAGCTGCA GCAGAAAGTC GATGAGTTAA
     3001 ATAAAGAAAT TCAGAAGGAG ATGGATCAAA GAGATGCCAT AACAAAAATG AAAGATGTCT
     3061 ACCTAAAGAA TCCTCAGATG GGAGACCCAG CCAGTTTGGA TCACAAATTA GCAGAAGTCA
     3121 GCCAAAATAT AGAGAAACTG CGAGTAGAGA CCCAGAAATT TGAGGCCTGG CTGGCTGAGG
     3181 TTGAAGGCCG GCTCCCAGCA CGCAGCGAGC AGGCGCGCCG GCAGAGCGGA CTGTACGACA
     3241 GCCAGAACCC ACCCACAGTC AACAACTGCG CCCAGGACCG TGAGAGCCCA GATGGCAGTT
     3301 ACACAGAGGA GCAGAGTCAG GAGAGTGAGA TGAAGGTGCT GGCCACGGAT TTTGACGACG
     3361 AGTTTGATGA TGAGGAGCCC CTCCCTGCCA TAGGGACGTG CAAAGCTCTC TACACATTTG
     3421 AAGGTCAGAA TGAAGGAACG ATTTCCGTAG TTGAAGGAGA AACATTGTAT GTCATAGAGG
     3481 AAGACAAAGG CGATGGCTGG ACCCGCATTC GGAGAAATGA AGATGAAGAG GGTTATGTCC
     3541 CCACTTCATA TGTCGAAGTC TGTTTGGACA AAAATGCCAA AGATTCCGGC CGCCTCGAGA
     3601 TGgtgagcaa gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg
     3661 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3721 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc tcctgggaca
     3781 tcctgtcccc tcagttcatg tacggctcca gggccttcat caagcacccc gccgacatcc
     3841 ccgactacta taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     3901 aggacggcgg cgccgtgacc gtgacccagg acacctccct ggaggacggc accctgatct
     3961 acaaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaga
     4021 caatgggctg ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg
     4081 acattaagat ggccctgcgc ctgaaggacg gcggAcgcta cctggcggac ttcaagacca
     4141 cctacaaggc caagaagccc gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg
     4201 acatcacctc ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgagggcc
     4261 gccactccac cggcggcatg gacgagctgt acaagTAAAG ATCTTTTTCC CTCTGCCAAA
     4321 AATTATGGGG ACATCATGAA GCCCCTTGAG CATCTGACTT CTGGCTAATA AAGGAAATTT
     4381 ATTTTCATTG CAATAGTGTG TTGGAATTTT TTGTGTCTCT CACTCGGAAG GACATATGGG
     4441 AGGGCAAATC ATTTAAAACA TCAGAATGAG TATTTGGTTT AGAGTTTGGC AACATATGCC
     4501 CATATGCTGG CTGCCATGAA CAAAGGTTGG CTATAAAGAG GTCATCAGTA TATGAAACAG
     4561 CCCCCTGCTG TCCATTCCTT ATTCCATAGA AAAGCCTTGA CTTGAGGTTA GATTTTTTTT
     4621 ATATTTTGTT TTGTGTTATT TTTTTCTTTA ACATCCCTAA AATTTTCCTT ACATGTTTTA
     4681 CTAGCCAGAT TTTTCCTCCT CTCCTGACTA CTCCCAGTCA TAGCTGTCCC TCTTCTCTTA
     4741 TGGAGATCCC TCGACCTGCA GCCCAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT
     4801 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA
     4861 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC
     4921 TTTCCAGTCG GGAAACCTGT CGTGCCAGCG GATCCGCATC TCAATTAGTC AGCAACCATA
     4981 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG
     5041 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG
     5101 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTAACT
     5161 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA
     5221 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC
     5281 ATGTCTGGAT CCGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
     5341 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
     5401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
     5461 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
     5521 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     5581 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     5641 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     5701 TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     5761 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     5821 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     5881 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     5941 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA
     6001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     6061 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     6121 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     6181 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     6241 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     6301 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     6361 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     6421 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     6481 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     6541 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     6601 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     6661 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     6721 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     6781 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     6841 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     6901 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     6961 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     7021 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     7081 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     7141 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     7201 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT
     7261 TCCCCGAAAA GTGCCACCTG
//