LOCUS       ___12_PB_CAG_F_b        9083 bp    DNA        circular     20-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     LOCUS GS56925 pBSK-seq56925 3680 bp DNA circular SYN 19-DEC-2014
            DEFINITION Ligation of inverted seq56925 into modified pBSK
            ACCESSION GS56925 pBSK-seq56925 KEYWORDS . SOURCE Unknown. ORGANISM
            Unknown Unclassified. REFERENCE 1 (bases 1 to 3680) AUTHORS Self
            JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager,
            Scientific & Educational Software COMMENT SECNOTES|Vector molecule:
            modified pBSK Fragment ends: SmaI Fragment size: 2848 Insert
            molecule: seq56925 Fragment ends: blunt Fragment size: 830
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             129..986
                     /locus_tag="Amp+"
                     /label="Amp+"
                     /ApEinfo_label="Amp+"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1137..1804)
                     /locus_tag="pUC origin"
                     /label="pUC origin"
                     /ApEinfo_label="pUC origin"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2200..2606)
                     /note="3' piggyBac TR"
                     /locus_tag="3' piggyBac TR"
                     /label="3' piggyBac TR"
                     /ApEinfo_label="3' piggyBac TR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     enhancer        2620..2997
                     /note="hCMV enhancer"
                     /locus_tag="hCMV enhancer"
                     /label="hCMV enhancer"
                     /ApEinfo_label="hCMV enhancer"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2943..2964)
                     /note="HN853"
                     /locus_tag="HN853"
                     /label="HN853"
                     /ApEinfo_label="HN853"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3003..3280
                     /note="Chicken b-actin promoter"
                     /locus_tag="Chicken b-actin promoter"
                     /label="Chicken b-actin promoter"
                     /ApEinfo_label="Chicken b-actin promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3190..3194
                     /note="CCATT box"
                     /locus_tag="CCATT box"
                     /label="CCATT box"
                     /ApEinfo_label="CCATT box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3252..3259
                     /note="TATA box"
                     /locus_tag="TATA box"
                     /label="TATA box"
                     /ApEinfo_label="TATA box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3264..3280
                     /note="TSS"
                     /locus_tag="TSS"
                     /label="TSS"
                     /ApEinfo_label="TSS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3281..3371
                     /note="Chicken b-actin exon 1"
                     /locus_tag="Chicken b-actin exon 1"
                     /label="Chicken b-actin exon 1"
                     /ApEinfo_label="Chicken b-actin exon 1"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3372..4296
                     /note="Ch b-actin Rb globin hybrid intron"
                     /locus_tag="Ch b-actin Rb globin hybrid intron"
                     /label="Ch b-actin Rb globin hybrid intron"
                     /ApEinfo_label="Ch b-actin Rb globin hybrid intron"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3372..4247
                     /note="Chicken b-actin intron"
                     /locus_tag="Chicken b-actin intron"
                     /label="Chicken b-actin intron"
                     /ApEinfo_label="Chicken b-actin intron"
                     /ApEinfo_fwdcolor="#eb606c"
                     /ApEinfo_revcolor="#eb606c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4248..4344
                     /note="Rabbit b-globin splice acceptor"
                     /locus_tag="Rabbit b-globin splice acceptor"
                     /label="Rabbit b-globin splice acceptor"
                     /ApEinfo_label="Rabbit b-globin splice acceptor"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4261..4281
                     /note="HN737"
                     /locus_tag="HN737"
                     /label="HN737"
                     /ApEinfo_label="HN737"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4305..4324
                     /note="HN382"
                     /locus_tag="HN382"
                     /label="HN382"
                     /ApEinfo_label="HN382"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4356..4389)
                     /note="FRT"
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4390..4688
                     /note="bGH pA"
                     /locus_tag="bGH pA"
                     /label="bGH pA"
                     /ApEinfo_label="bGH pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4713..4940
                     /note="SV40 pA"
                     /locus_tag="SV40 pA"
                     /label="SV40 pA"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4941..4951
                     /note="3x STOP"
                     /locus_tag="3x STOP"
                     /label="3x STOP"
                     /ApEinfo_label="3x STOP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4952..4985)
                     /note="FRT"
                     /locus_tag="FRT(1)"
                     /label="FRT(1)"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4978..4995
                     /note="HN1149"
                     /locus_tag="HN1149"
                     /label="HN1149"
                     /ApEinfo_label="HN1149"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4998..5031)
                     /note="loxP"
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5032..5330
                     /note="bGH pA"
                     /locus_tag="bGH pA(1)"
                     /label="bGH pA(1)"
                     /ApEinfo_label="bGH pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5355..5582
                     /note="SV40 pA"
                     /locus_tag="SV40 pA(1)"
                     /label="SV40 pA(1)"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5583..5593
                     /note="3x STOP"
                     /locus_tag="3x STOP(1)"
                     /label="3x STOP(1)"
                     /ApEinfo_label="3x STOP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5600..5633)
                     /note="loxP"
                     /locus_tag="loxP(1)"
                     /label="loxP(1)"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5643..7103
                     /note="tdGFP"
                     /locus_tag="tdGFP"
                     /label="tdGFP"
                     /ApEinfo_label="tdGFP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5643..6356
                     /note="superfolder GFP"
                     /locus_tag="superfolder GFP"
                     /label="superfolder GFP"
                     /ApEinfo_label="superfolder GFP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6357..6386
                     /note="linker"
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7112..7697
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7718..8019
                     /note="bGH pA"
                     /locus_tag="bGH pA(2)"
                     /label="bGH pA(2)"
                     /ApEinfo_label="bGH pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(8026..8432)
                     /note="5' piggyBac TR"
                     /locus_tag="5' piggyBac TR"
                     /label="5' piggyBac TR"
                     /ApEinfo_label="5' piggyBac TR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             8469..8624
                     /locus_tag="lacZ"
                     /label="lacZ"
                     /ApEinfo_label="lacZ"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(8757..9063)
                     /locus_tag="f1 origin"
                     /label="f1 origin"
                     /ApEinfo_label="f1 origin"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacctccccc ggtattcacg acagcaggct
     2221 gaataataaa aaaattagaa actattattt aaccctagaa agataatcat attgtgacgt
     2281 acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg tatatcgagg
     2341 tttatttatt aatttgaata gatattaagt tttattatat ttacacttac atactaataa
     2401 taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa
     2461 aatttcttct ataaagtaac aaaactttta aacattctct cttttacaaa aataaactta
     2521 ttttgtactt taaaaacagt catgttgtat tataaaataa gtaattagct taacctatac
     2581 ataatagaaa caaattatac ttattaaccg gtcatggtcg acattgatta ttgactagtt
     2641 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     2701 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2761 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2821 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2881 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2941 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3001 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     3061 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
     3121 ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg
     3181 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
     3241 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc
     3301 cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     3361 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     3421 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
     3481 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     3541 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     3601 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     3661 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     3721 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc
     3781 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     3841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     3901 gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     3961 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     4021 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     4081 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     4141 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     4201 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     4261 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     4321 gtgctgtctc atcattttgg caaagaattg ctagcgaagt tcctatactt tctagagaat
     4381 aggaacttca gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4441 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4501 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4561 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4621 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcttgcgg
     4681 aacccttacc accgcggtgg cggccgctct agaatcataa tcagccatac cacatttgta
     4741 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     4801 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4921 aaactcatca atgtatctta taactagata agaagttcct atactttcta gagaatagga
     4981 acttcgctag cacgcgtata acttcgtata gcatacatta tacgaagtta tagctcgctg
     5041 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5101 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5161 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5221 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5281 tgaggcggaa agaaccagct ggggctcgac tagagcttgc ggaaccctta ccaccgcggt
     5341 ggcggccgct ctagaatcat aatcagccat accacatttg tagaggtttt acttgcttta
     5401 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     5461 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5521 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5581 tataactaga taacctagga taacttcgta tagcatacat tatacgaagt tataggcgcg
     5641 ccatgagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     5701 gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg
     5761 gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac
     5821 ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac
     5881 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
     5941 tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
     6001 ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     6061 aactcgaata caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
     6121 gaatcaaagc taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag
     6181 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     6241 acctgtcgac acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc
     6301 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaaggtt
     6361 ctgctggatc tgcagcagga tctggtatga gcaaaggaga agaacttttc actggagttg
     6421 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag
     6481 agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc actactggaa
     6541 aactacctgt tccgtggcca acacttgtca ctactctgac ctatggtgtt caatgctttt
     6601 cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
     6661 atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg cgtgctgaag
     6721 tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaagggtatt gattttaaag
     6781 aagatggaaa cattcttgga cacaaactcg aatacaactt taactcacac aatgtataca
     6841 tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacgttg
     6901 aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     6961 ctgtcctttt accagacaac cattacctgt cgacacaatc tgtcctttcg aaagatccca
     7021 acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg
     7081 gcatggatga gctctacaaa tgattaatta aaacctctgg attacaaaat ttgtgaaaga
     7141 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7201 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7261 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7321 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7381 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7441 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7501 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7561 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7621 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7681 tgggccgcct ccccgcagga tccactagag cggccgccta gagctcgctg atcagcctcg
     7741 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7801 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7861 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     7921 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     7981 agaaccagct ggggctcgac tagagcttgc ggaaccctta agctttttta ttatatatta
     8041 gtcacgatat ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg
     8101 aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg
     8161 cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca
     8221 agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat
     8281 tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga
     8341 ctatctttct agggttaaaa aagatttgcg ctttactcga cctaaacttt aaacacgtca
     8401 tagaatcttc gtttgacaaa aaccacattg tgttgggtcg ccctatagtg agtcgtatta
     8461 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8521 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8581 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg taagcgttaa
     8641 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     8701 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     8761 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     8821 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     8881 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     8941 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     9001 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     9061 tgcgccgcta cagggcgcgt cag   
//
