LOCUS       Exported                4957 bp ds-DNA     circular SYN 10-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 29, 2021 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(134..136,614..619)
                     /organism="Cloning vector pTurbo-Cre"
                     /mol_type="other DNA"
                     /label=source:Cloning vector pTurbo-Cre
                     /label=source:Cloning vector pTurbo-Cre(1)
                     /db_xref="taxon:152298"
     source          614..619
                     /organism="Cloning vector pTurbo-Cre"
                     /mol_type="other DNA"
                     /label=source:Cloning vector pTurbo-Cre
                     /db_xref="taxon:152298"
     source          626..1390
                     /organism="synthetic construct"
                     /mol_type="mRNA"
                     /label=source:synthetic construct
                     /db_xref="taxon:32630"
     misc_feature    1..130
                     /label=L-ITR
                     /note="/vntifkey=21"
     misc_feature    14..29
                     /label=AAV REP78 binding element
     misc_feature    38..46
                     /label=AAV Hairpin element 2
                     /label=AAV Hairpin element 2(2)
     misc_feature    complement(47)
                     /label=AAV Hairpin element 2
                     /label=AAV Hairpin element 2(3)
     misc_feature    49..57
                     /label=AAV Hairpin element 1
     misc_feature    complement(61..69)
                     /label=AAV Hairpin element 1
                     /label=AAV Hairpin element 1(1)
     misc_feature    complement(78..93)
                     /label=AAV REP78 binding element
                     /label=AAV REP78 binding element(1)
     misc_feature    110..130
                     /label=AAV D' element
     misc_feature    137..613
                     /label=synapsin-1 promoter from pBOB-synP
     primer_bind     619..637
                     /label=itTA-fp-57
     misc_feature    620..625
                     /label=Kozak sequence
     CDS             626..1390
                     /codon_start=1
                     /transl_table=11
                     /product="improved tetracycline-responsive activator"
                     /label=improved tetracycline-responsive activator
                     /note="itTA"
                     /db_xref="GI:89277221"
                     /protein_id="ABD66750.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSHVPKKRKHVPADALD
                     DFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     misc_feature    626..1390
                     /label=itTA from Shimshek et al. '05
     primer_bind     complement(1292..1312)
                     /label=itTA-rp-57
     primer_bind     complement(1370..1393)
                     /label=itTA-rp-57
     misc_feature    1394..1401
                     /label=NotI
     misc_feature    1402..1993
                     /label=WPRE (match to WHV8 genome, J04514)
     misc_feature    1994..2218
                     /label=BGH pA
     misc_feature    1994
                     /label=BamHI site
     misc_feature    2220..2360
                     /label=R-ITR
                     /note="/vntifkey=21"
     misc_feature    complement(2220..2240)
                     /label=AAV D' element
                     /label=AAV D' element(1)
     misc_feature    2257..2272
                     /label=AAV REP78 binding element
                     /label=AAV REP78 binding element(2)
     misc_feature    2281..2289
                     /label=AAV Hairpin element 1
                     /label=AAV Hairpin element 1(2)
     misc_feature    complement(2293..2301)
                     /label=AAV Hairpin element 1
                     /label=AAV Hairpin element 1(3)
     misc_feature    2303..2311
                     /label=AAV Hairpin element 2
     misc_feature    complement(2315..2323)
                     /label=AAV Hairpin element 2
                     /label=AAV Hairpin element 2(1)
     misc_feature    complement(2332..2347)
                     /label=AAV REP78 binding element
                     /label=AAV REP78 binding element(3)
     rep_origin      2435..2890
                     /label=M13 origin
     rep_origin      2452..2758
                     /label=F1 ori
     rep_origin      2452..2758
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    3277..4134
                     /label=AmpR
                     /note="/vntifkey=21"
     CDS             3475..4134
                     /label=AmpR
                     /label=AmpR(1)
     rep_origin      4232..4914
                     /label=ColE1 origin
     rep_origin      4285..4952
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gtcgacgcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta
      181 ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca
      241 agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca
      301 ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc
      361 cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg
      421 gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag
      481 ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg
      541 gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc
      601 tgagagcgca gtcgaattcg ccaccatgtc cagactggac aagagcaaag tcatcaactc
      661 tgccttggag ctcctgaatg aagttggcat tgagggcttg accaccagga agctggccca
      721 gaagctgggt gtggagcagc ctaccctgta ctggcatgtg aagaacaaga gggctctgct
      781 tgatgccctg gccattgaga tgttggacag gcaccacacc cacttctgcc ctctggaagg
      841 ggagtcctgg caggacttcc tgaggaacaa tgccaagagc ttcagatgtg ccttgctctc
      901 ccaccgggat ggtgccaaag ttcacttggg caccaggcct acagagaagc agtatgagac
      961 cctggagaac cagctggcat tcctgtgcca acaaggcttc tccctggaaa atgccttgta
     1021 tgccctctct gctgtgggcc acttcacctt gggctgtgtg ctggaggacc aggagcacca
     1081 agttgccaag gaggagaggg agacccccac cactgactcc atgccaccac tgctgcggca
     1141 agctattgag ttgtttgacc accaaggggc tgagcctgca ttcctttttg gcctggaact
     1201 gatcatctgt ggcctggaaa agcagctgaa atgtgagtct ggctctcacg tgcccaaaaa
     1261 gagaaagcac gtgcctgctg atgccttgga tgattttgac ctggacatgc tgcctgctga
     1321 tgccctggat gactttgatt tggacatgct ccctgctgat gcacttgatg attttgacct
     1381 ggatatgctg tgagcggccg caatcaacct ctggattaca aaatttgtga aagattgact
     1441 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     1501 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     1561 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     1621 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     1681 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     1741 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     1801 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     1861 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     1921 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     1981 gcctccccgc ctgctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2041 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2101 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2161 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggca
     2221 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     2281 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     2341 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2401 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     2461 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2521 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2581 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2641 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2701 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2761 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2821 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2881 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2941 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3001 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3061 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3121 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3181 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3241 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3301 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3361 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3421 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3481 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3541 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3601 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3661 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3721 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3781 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3841 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3901 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3961 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4021 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4081 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4141 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4201 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4381 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4441 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4621 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4921 cggttcctgg ccttttgctg gccttttgct cacatgt
//
