LOCUS       pLX_HA_c7orf26v2        9457 bp    DNA     circular UNA 29-OCT-2021
DEFINITION  an expression clone created from pLX_HA and pDONR221 - RC219786
            extraction PCR Product Entry clone.
ACCESSION   urn.local...u-e1a9kpd
VERSION     urn.local...u-e1a9kpd
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     1..27
                     /standard_name="ha_triple_stop_F"
     misc_binding    51..639
                     /note="WPRE"
     LTR             710..945
                     /standard_name="3'\LTR\SIN"
     polyA_signal    1022..1152
                     /note="SV40 polyadenylation signal"
     rep_origin      1232..1307
     rep_origin      1525..1980
     CDS             2111..>2390
                     /note="AmpR"
                     /standard_name="AmpR CDS"
     CDS             <2365..2971
                     /codon_start=2
                     /note="AmpR"
                     /standard_name="AmpR CDS"
     primer_bind     2365..2390
                     /standard_name="753_ampR_F"
     primer_bind     complement(2365..2390)
                     /standard_name="754_ampR_R"
     rep_origin      3119..3786
     LTR             4195..4607
                     /standard_name="5'LTR"
     misc_feature    4658..4795
                     /standard_name="psi"
     misc_feature    4762..5126
                     /standard_name="gag"
     misc_feature    5272..5513
                     /standard_name="RRE"
     promoter        5914..6188
                     /note="SV40"
                     /standard_name="SV40 promoter"
     CDS             6214..6816
                     /note="PuroR"
                     /standard_name="PuroR CDS"
     misc_feature    6859..6976
                     /standard_name="cPPT"
     promoter        7034..8293
                     /note="EF1a"
                     /standard_name="EF1a promoter"
     misc_feature    8312..>8319
                     /standard_name="attR1"
     misc_feature    8313..8338
                     /note="Geneious type: attB1"
                     /standard_name="attB1"
     misc_feature    8339..8341
                     /standard_name="Kozak"
     primer_bind     8342..8362
                     /standard_name="1 F"
     primer_bind     8342..8362
                     /standard_name="pFastBac-C7orf26-F"
     primer_bind     8342..8361
                     /standard_name="1 F"
     misc_feature    <8342..9389
                     /standard_name="C7orf26"""
     misc_feature    8341^8342
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GCGATCGCC"
     misc_feature    8533^8534
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="AGACTGAATTCCCTTCAGGAGCTTCAACTTCTTGAAATCATG
                     TGCAATTATTTCCAG"
     misc_feature    8926^8927
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="AGGTTGATTCTCATCACTTTTTTAAATACTCCGATTGCGGCC
                     AATCTGCCAATAGGATTCTTAGAGCTCACCCCGCTCGTTGGATTGATCCGCTGGTGCG
                     TGAAGGCACCCCTGGCTTATAAAAGGAAAAAGAAGCCCCCCTTATCCAATGGCCATGT
                     CAGCAACAAGGTCACAAAGGACCCGGGCGTGGGGATGGACAGAGACTCCCACCTCTTG
                     TACTCAAAACTCCACCTC"
     primer_bind     complement(9378..9397)
                     /standard_name="1,347 R"
     primer_bind     complement(9378..9397)
                     /standard_name="pFastBac-C7orf26-R_no_stop"
     misc_feature    9389..9395
                     /standard_name="Restriction Enzyme"""
     misc_feature    complement(9398..9422)
                     /note="Geneious type: attB2"
                     /standard_name="attB2"
     misc_feature    <9409..9423
                     /standard_name="attR2"
     misc_feature    <9431..9457
                     /standard_name="Wheeler_HA"
     misc_feature    complement(9431..9457)
                     /standard_name="HA_rev"
     misc_feature    9431..9457
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
ORIGIN      
        1 tagtaatgaa ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac aatcaacctc
       61 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
      121 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
      181 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
      241 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
      301 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
      361 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
      421 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
      481 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
      541 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
      601 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
      661 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
      721 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
      781 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      841 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      901 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
      961 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     1021 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1081 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1141 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     1201 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1261 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     1321 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     1381 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1441 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1501 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1561 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     1621 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     1681 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     1741 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     1801 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     1861 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     1921 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     1981 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2041 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2101 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2161 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2221 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2281 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2341 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2401 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2461 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2521 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2581 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2641 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2701 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2761 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2821 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2881 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2941 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3001 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3061 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3781 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3841 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3901 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3961 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4021 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4081 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     4141 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     4201 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     4261 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     4321 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     4381 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     4441 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4501 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4561 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4621 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4681 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4741 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4801 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4861 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4921 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4981 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     5041 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     5101 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     5161 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     5221 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5281 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5341 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5401 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5461 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5521 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5581 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5641 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     5701 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     5761 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     5821 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     5881 acccacctcc caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt
     5941 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt
     6001 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6061 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6121 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6181 aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc cacggtgcgc
     6241 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6301 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6361 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6421 ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6481 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6541 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6601 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6661 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6721 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6781 acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct tgagacaaat
     6841 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     6901 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     6961 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     7021 cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     7081 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     7141 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     7201 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     7261 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     7321 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     7381 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     7441 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     7501 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     7561 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     7621 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     7681 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     7741 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     7801 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     7861 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     7921 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     7981 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     8041 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     8101 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     8161 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     8221 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     8281 caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag caggcttaac
     8341 catgagcgac atccgccact cgctgctgcg ccgcgatgcg ctgagcgccg ccaaggaggt
     8401 gttgtaccac ctggacatct acttcagcag ccagctgcag agcgcgccgc tgcccatcgt
     8461 ggacaagggc cccgtggagc tgctggagga gttcgtgttc caggtgccca aggagcgcag
     8521 cgcgcagccc aaggagcaaa ccaaggactc tgttcggcag attatttttt catccctttt
     8581 cagccctcaa gggaacaaag ccgatgacag ccggatgagc ttgttgggaa aactggtctc
     8641 catggcggtg gctgtgtgtc gaatcccggt gttggagtgt gctgcctcct ggcttcagcg
     8701 gacgcccgtg gtttactgtg tgaggttagc caaggccctt gtagatgact actgctgttt
     8761 ggtgccggga tccattcaga cgctgaagca gatattcagt gccagcccga gattctgctg
     8821 ccagttcatc acctccgtta ccgcgctcta tgacctgtca tcagatgacc tcattccacc
     8881 tatggacttg cttgaaatga ttgtcacctg gatttttgag gacccaagcg tcctgcaagt
     8941 gctcatgacg ctgcagctgc acctgaccga gaagaatctg tatgggcgcc tggggctgat
     9001 cctcttcgac cacatggtcc cgctggtaga ggagatcaac aggttggcgg atgaactgaa
     9061 ccccctcaac gcctcccagg agattgagct ctcgctggac cggctggcgc aggctctgca
     9121 ggtggccatg gcctcaggag ctctgctgtg cacgagagat gacctgagaa ccttgtgctc
     9181 caggctgccc cataataacc tcctccagct ggtgatctcg ggtcccgtgc agcagtcgcc
     9241 tcacgccgcg ctccccccgg ggttctaccc ccacatccac acgcccccgc tgggctacgg
     9301 ggctgtcccg gcccaccccg ccgcccaccc cgccctgccc acgcaccccg gccacacctt
     9361 catctccggc gtgacctttc ccttcaggcc catccgctac ccagctttct tgtacaaagt
     9421 ggttgatatc tacccatacg atgttccaga ttacgct
//
