LOCUS       Exported               11847 bp ds-DNA     circular SYN 27-JAN-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Justin Lab
  TITLE     Direct Submission
  JOURNAL   Exported Feb 10, 2022 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     2594..2630
                     /label=Primer 1
                     /note="color: black; sequence: 
                     GAAAATTAATTAAAGATCTAACATATCCTGGTGTGGAGTAGG"
     misc_feature    2598
                     /label=CaMKIIa promoter
                     /note="color: #ccffcc"
     misc_feature    2602..2607
                     /label=BglII
                     /note="BglII"
                     /note="color: #ffff99"
     misc_feature    2608..3292
                     /label=GFAP promoter
                     /note="GFAP promoter"
                     /note="color: #cc99ff"
     primer_bind     complement(3282..3299)
                     /label=Primer 2
                     /note="color: black; sequence: GAAAAGGATCCCCGCGAGCAG"
     CDS             3321..4814
                     /codon_start=1
                     /label=PHR
                     /note="color: #00ffff"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     4469..4491
                     /label=PHR-D387A-F
                     /note="color: black; sequence: TACCCTTCTCGCCGCCGACTTGG"
     primer_bind     complement(4469..4491)
                     /label=PHR-D387A-R
                     /note="color: black; sequence: CCAAGTCGGCGGCGAGAAGGGTA"
     primer_bind     4743..4758
                     /label=PHR-CF
                     /note="color: black; sequence: GATACAGCACGCGAGC"
     primer_bind     4801..4827
                     /label=CRY2PHR-InsMu-F
                     /note="color: black; sequence: TGATCGGAGCAGCGGgCAGCGGCgcca"
     CDS             4824..4880
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     4847..4906
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: 
                     gcaagcaggagacgtggaagaaaaccccggtcctATGAATGGAGCTATAGGAGGTGACC
                     T"
     CDS             4881..5390
                     /codon_start=1
                     /label=CIBN
                     /note="color: #993366"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     primer_bind     5404..5422
                     /label=Primer 1
                     /note="color: black; sequence: 
                     GAAAAGCTAGCCACCCCACCATGGTGAGCAAGGG"
     CDS             5409..6116
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     5564..5575
                     /label=Primer 1
                     /note="color: black; sequence: 
                     GAAAAGCTAGCCACCCCACCATGGTGAGCAAGGG"
     primer_bind     complement(6096..6116)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     GCTTGATATCGAATTCttacttgtacagctcgtccatgcc"
     misc_feature    6126..6139
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     CDS             6147..6494
                     /codon_start=1
                     /label=VAMP2
                     /note="color: #3366ff"
                     /translation="MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDI
                     MRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICA
                     IILIIIIVYFSS"
     primer_bind     complement(6462..6506)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     GAAGCTTGAGCTCGAGGGCGCGCCTTAAGAGCTgaagtaaactatgatgatgatgagga
                     t"
     primer_bind     complement(6502..6530)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     GCTTGATATCGAATTCccctgcaggggcg"
     misc_feature    6539..7127
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7010..7021)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7009"
                     /translation="IEGR"
     primer_bind     complement(7130..7146)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             7652..7832
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    7864..8088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      8134..8562
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        8576..8905
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      8756..8891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        8953..9000
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             9019..9393
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(9226..9243)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     GCTTGATATCGAATTCttacttgtacagctcgtccatgcc"
     polyA_signal    9523..9644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(9693..9709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    9717..9733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9741..9771)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9741 .. 9747 / #ffffff / -10
                        2: 9748 .. 9765 / #ffffff
                        3: 9766 .. 9771 / #ffffff / -35"
     protein_bind    9786..9807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(10095..10683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10854..11714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10854 .. 11645 / #ccffcc
                        2: 11646 .. 11714 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11715..11819)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagatctaac atatcctggt gtggagtagg ggacgctgct
     2641 ctgacagagg ctcgggggcc tgagctggct ctgtgagctg gggaggaggc agacagccag
     2701 gcctttgtct gcaagcagac ctggcagcat tgggctggcc gccccccagg gcctcctctt
     2761 catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg cacagtgcct
     2821 gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt gagcaggggg
     2881 cttgcattgc accccagcct gacagcctgg catcttggga taaaagcagc acagccccct
     2941 aggggctgcc cttgctgtgt ggcgccaccg gcggtggaga acaaggctct attcagcctg
     3001 tgcccaggaa aggggatcag gggatgccca ggcatggaca gtgggtggca gggggggaga
     3061 ggagggctgt ctgcttccca gaagtccaag gacacaaatg ggtgagggga gagctctccc
     3121 catagctggg ctgcggccca accccacccc ctcaggctat gccagggggt gttgccaggg
     3181 gcacccgggc atcgccagtc tagcccactc cttcataaag ccctcgcatc ccaggagcga
     3241 gcagagccag agcaggttgg agaggagacg catcacctcc gctgctcgcg gggatccttc
     3301 gaaaccggtg ctagcccacc atgaagatgg acaaaaagac catcgtctgg tttcggagag
     3361 atttgagaat agaagataat cccgcgctcg ccgccgcggc ccacgagggt tccgtcttcc
     3421 ccgttttcat ttggtgtcct gaagaagaag gccagtttta tcccggaagg gcctctaggt
     3481 ggtggatgaa gcaaagtctg gcccatctta gccagtcact gaaagcactg ggcagtgatc
     3541 ttaccctgat caagacacac aataccatct ctgccatcct cgactgcatc agggtgaccg
     3601 gcgcaacgaa agtcgtgttt aaccacctgt acgatccagt tagtctggtg cgcgaccaca
     3661 ctgtgaagga gaagctggtg gaacggggga tcagtgtgca gagctacaac ggggaccttc
     3721 tgtacgagcc atgggagatc tattgcgaga aagggaaacc gttcacctcc ttcaacagtt
     3781 actggaagaa atgtttggat atgtcaatag agtccgttat gttgccccct ccctggagac
     3841 tgatgccgat tactgctgct gcagaggcca tctgggcctg ctccatcgag gaactcggtc
     3901 tggaaaatga agcagaaaag ccaagcaatg cacttctcac tagagcctgg agccccggct
     3961 ggtctaatgc cgacaagctg cttaacgagt tcatcgaaaa acaactgatt gactacgcga
     4021 agaactccaa gaaagtggta ggtaactcaa ctagcttgct ctctccatat ctccattttg
     4081 gcgagatttc tgtccgccat gtatttcagt gcgctcggat gaaacagatt atctgggctc
     4141 gcgataaaaa cagcgaaggc gaagaaagcg ccgatctgtt cctgcgaggg atcggacttc
     4201 gggaatactc ccggtatata tgtttcaact ttccattcac acacgagcag agtctgttgt
     4261 cccacctcag gttcttcccc tgggacgccg atgtcgacaa attcaaggca tggagacagg
     4321 gaaggacagg gtacccactc gtggatgctg gcatgagaga gctctgggct acaggctgga
     4381 tgcacaaccg catccgggta atcgtgtcct catttgctgt caagtttctg ctcctgcctt
     4441 ggaaatgggg aatgaagtac ttttgggata cccttctcga cgccgacttg gagtgtgaca
     4501 ttctgggatg gcaatatatt agcgggtcaa ttcctgacgg ccatgagttg gacaggttgg
     4561 acaatccggc cttgcaggga gctaagtatg atcccgaagg agagtatatt cgacagtggc
     4621 tccccgagct ggcccgactt cctacggagt ggattcacca tccttgggac gcaccactga
     4681 cagtgctcaa ggcaagcggg gtggagctgg gcaccaatta cgctaagcct atagttgata
     4741 tagatacagc acgcgagctg ctggctaaag cgatctctcg cactcgggag gcgcagatta
     4801 tgatcggtgc tgccggcagc ggcgccacga acttctctct gttaaagcaa gcaggagacg
     4861 tggaagaaaa ccccggtcct atgaatggag ctataggagg tgaccttttg ctcaattttc
     4921 ctgacatgtc ggtcctagag cgccaaaggg ctcacctcaa gtacctcaat cccacctttg
     4981 attctcctct cgccggcttc tttgccgatt cttcaatgat taccggcggc gagatggaca
     5041 gctatctttc gactgccggt ttgaatcttc cgatgatgta cggtgagacg acggtggaag
     5101 gtgattcaag actctcaatt tcgccggaaa cgacgcttgg gactggaaat ttcaaggcag
     5161 cgaagtttga tacagagact aaggattgta atgaggcggc gaagaagatg acgatgaaca
     5221 gagatgacct agtagaagaa ggagaagaag agaagtcgaa aataacagag caaaacaatg
     5281 ggagcacaaa aagcatcaag aagatgaaac acaaagccaa gaaagaagag aacaatttct
     5341 ctaatgattc atctaaagtg acgaaggaat tggagaaaac ggattatatt catgtaccgg
     5401 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     5461 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     5521 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     5581 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     5641 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     5701 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     5761 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     5821 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     5881 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     5941 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     6001 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     6061 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggca
     6121 gtggttccgg actcagatct cgagctatgt cggctaccgc tgccaccgtc ccgcctgccg
     6181 ccccggccgg cgagggtggc ccccctgcac ctcctccaaa ccttactagt aacaggagac
     6241 tgcagcagac ccaggcccag gtggatgagg tggtggacat catgagggtg aatgtggaca
     6301 aggtcctgga gcgggaccag aagttgtcgg agctggatga ccgtgcagat gccctccagg
     6361 caggggcctc ccagtttgaa acaagtgcag ccaagctcaa gcgcaaatac tggtggaaaa
     6421 acctcaagat gatgatcatc ttgggagtga tctgcgccat catcctcatc atcatcatag
     6481 tttacttcag ctcttaaggc gcgcccctgc aggggaattc gatatcaagc ttatcgataa
     6541 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6601 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6661 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6721 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6781 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6841 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6901 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6961 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7021 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7081 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     7141 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     7201 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     7261 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7321 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     7381 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     7441 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     7501 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     7561 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     7621 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     7681 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7741 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7801 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     7861 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7921 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7981 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     8041 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     8101 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     8161 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     8221 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     8281 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8341 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8401 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8461 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8521 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     8581 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8641 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8701 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8761 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8821 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     8881 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     8941 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     9001 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     9061 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     9121 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     9181 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     9241 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     9301 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     9361 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     9421 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     9481 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     9541 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9601 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     9661 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9721 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9781 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9841 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9901 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9961 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    10021 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    10081 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10141 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10201 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10261 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10321 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10381 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10441 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10501 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    10561 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10621 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10681 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10741 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10801 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10861 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10921 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10981 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    11041 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    11101 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    11161 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    11221 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    11281 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    11341 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    11401 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    11461 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11521 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11581 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11641 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11701 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11761 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11821 acatttcccc gaaaagtgcc acctgac
//
