LOCUS       pAc2_gRNA1              1851 bp ds-DNA     circular     30-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    368..402
                     /label="J23119"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    403..430
                     /label="Ac2 I-E repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    431..463
                     /label="Ac2 I-E spacer1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    464..491
                     /label="Ac2 I-E repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    492..530
                     /label="Terminator"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    complement(559..667)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(668..1327)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     promoter        complement(1328..1432)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(join(1433..1851,1..345))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    join(1508..1851,1..246)
                     /label="pBR322 ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
       61 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtCGAGA GCATCGATCC
      361 TAGCATGttg acagctagct cagtcctagg tataatgcta gcGTCTTCCC CACGCCCGTG
      421 GGGGTGTTTC AATTCTGGCG AATCCTttaa ttaaCTGACC AGCGTCTTCC CCACGCCCGT
      481 GGGGGTGTTT Caaaaaaaaa ccccgcccct gacagggcgg ggtttttttt AAAGAATCGA
      541 TCGATCCCGA ATGAGTAGac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      601 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
      661 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
      721 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
      781 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
      841 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
      901 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
      961 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1021 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1081 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     1141 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1201 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1261 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1321 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1381 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc
     1441 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1501 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1561 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1621 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1681 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1741 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1801 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc c
//