LOCUS       pAc3_Cas7               6266 bp ds-DNA     circular     08-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(775..1857)
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        complement(1858..1935)
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        2244..2262
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    2263..2287
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    2329..3363
                     /label="Ac3 Cas7"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3370..3387
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      3454..3501
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3538..3993
                     /label="f1 origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(4086..4901)
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      5023..5611
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(6041..6232)
                     /label="rop"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
ORIGIN
        1 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
       61 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
      121 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
      181 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
      241 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
      301 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
      361 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
      421 atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
      481 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
      541 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
      601 gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg
      661 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
      721 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
      781 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      841 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
      901 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
      961 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     1021 gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga
     1081 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     1141 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     1201 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     1261 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     1321 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
     1381 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     1441 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     1501 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     1561 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     1621 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     1681 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     1741 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     1801 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     1861 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
     1921 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
     1981 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
     2041 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     2101 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     2161 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     2221 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
     2281 caattcccct ctagataact catatttgaa tagctaggga ggtttattAT GACTAGATTT
     2341 GTGCAGCTTC ATCTTCTCAT CTCTTATCCA CCAGCCAATC TGAATCGTGA TGACTTGGGT
     2401 CAGCCTAAAA CGGCAAGACT TGGGGGCGTC GAACGTCTGC GTGTGAGTTC TCAAAGCCTC
     2461 AAAAGAGCGT GGCGCACATC CGAGCTGTTT CAGCAGCAGT TGGCTGGATC AATTGGCACT
     2521 CGTACTAAAC GTCTCGGTAG AGAAGCTTTC GATGCACTCA TTGCTGCCGG CATAGGCGAA
     2581 AAGCAGGCTA CAGAATGGGC GAGCCAGATT GCCAAAGTGT ATGGAGCAGT AAAAAAGGAT
     2641 AATCCGTTAG AGATTGAGCA GTTAGTACAT ATAGCACCGG AAGAGCGCGC ATCACTAGGA
     2701 TCGCTGGTTG CAATTCTCGC CCGTGAAAAA AGAGCCCCAT CGGACGAAGA GCTGGATGCG
     2761 CTTCTACACA AGCAGTCGGC TGTAGACATT GCGATGTTTG GTCGTATGCT GGCTGCAAAG
     2821 ACGGAATACA ACGGGGATGC GGCGGTTCAG GTTGCACACG CAATTGGTGT TCATGCAAGT
     2881 GCTATAGAAG ATGATTATTT TACCGCAGTC GATGACCTGA ATACGGATGA CCCGGGTGCA
     2941 GCACACATTG GTGAATCCGG TTTTGCGGCG GCCGTGTTTT ATCAGTATCT TTGCATCGAT
     3001 AGAGATCTGT TGAAACGTAA CCTTGGCGGT GACGAGGAAC TGACTCAGAA AGCCCTGATA
     3061 GCTTTGGCTG AAGCAGCGCT GAAAGTGGGC CCGAGCGGCA AGCAAAACTC ATTTGCTTCA
     3121 CGAGCATACG CCCATTATGC ACTCGCCGAA AAGGGTCCTC AGCAGCCCCG GTCCTTAAGC
     3181 CTGGCCTTCA TTAAGCCTGT GTCTGGGGGC GACTATGCAT CAGAAGCCGT CGAAGTCTTG
     3241 CAGCGTTTGC GCGACAACAT GGATAAGGTG TATGGGGCAT GCGCGGATTC ACGTTATCAA
     3301 TTTGATGTTT TAAAAGGAGA GGGTTCTCTT GCCCAACTGT TAGATTTTGT GGCAGCCGAG
     3361 TAActcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
     3421 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     3481 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
     3541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3841 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3961 cgaattttaa caaaatatta acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc
     4021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt
     4081 aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     4141 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     4201 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     4261 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     4321 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     4381 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     4441 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     4501 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     4561 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
     4621 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     4681 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     4741 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     4801 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     4861 cgcggcctag agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
     4921 tttatgtaag cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt
     4981 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5041 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5101 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5161 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5221 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5281 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5401 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5641 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5701 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5761 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     5821 gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat
     5881 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     5941 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     6001 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     6061 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     6121 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     6181 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     6241 ccgtgtaagg gggatttctg ttcatg
//