LOCUS       pLm_gRNA1               1852 bp ds-DNA     circular     09-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    362..396
                     /label="J23119"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    397..425
                     /label="Lm I-E repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    426..457
                     /label="Lm I-E spacer 1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    458..486
                     /label="Lm I-E repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    487..525
                     /label="Terminator"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    complement(554..662)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(663..1322)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     promoter        complement(1323..1427)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(join(1428..1852,1..339))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    join(1503..1852,1..240)
                     /label="pBR322 ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
       61 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      301 ttctttcctg cgttatcccc tgattctgtg gataaccgtC GAGAGCATCG ATCCTAGCAT
      361 Gttgacagct agctcagtcc taggtataat gctagcGTGG TCCCCGCGCT GGCGGGGATG
      421 AACCGAATTC TGGCGAATCC TTTAATTAAC TGACCAGGTG GTCCCCGCGC TGGCGGGGAT
      481 GAACCGaaaa aaaaaccccg cccctgacag ggcggggttt tttttAAAGA ATCGATCGAT
      541 CCCGAATGAG TAGaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      601 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      661 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      721 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      781 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
      841 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
      901 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
      961 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1021 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1081 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1141 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1201 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1261 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     1321 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1381 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     1441 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1501 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1561 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1621 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1681 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1741 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1801 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tg
//