LOCUS       pXalb_IF1_Cascade       8668 bp ds-DNA     circular     09-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     gene            complement(793..1452)
                     /label="CAT/CamR"
                     /ApEinfo_revcolor=#00a600
                     /ApEinfo_fwdcolor=#00a600
     rep_origin      1814..2383
                     /label="p15A_origin"
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_fwdcolor=#808080
     rep_origin      2384..2653
                     /label="p15A_origin"
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_fwdcolor=#808080
     promoter        2812..2846
                     /label="J23119(SpeI) promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     RBS             3006..3017
                     /label="RBS (strong)"
                     /ApEinfo_revcolor=#996633
                     /ApEinfo_fwdcolor=#996633
     misc_feature    3024..4391
                     /label="I-F1 Cas8"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4388..5371
                     /label="I-F1 Cas5"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5398..6447
                     /label="I-F1 Cas7"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    6457..7023
                     /label="I-F1 Cas6"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      7042..7170
                     /label="dblTerm"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    7053..7096
                     /label="rrnB_T1_Terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7291..7448
                     /label="rrnB terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    7414..7457
                     /label="rrnB_T1_Terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7562..7719
                     /label="rrnB terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    7685..7728
                     /label="rrnB_T1_Terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      7860..7887
                     /label="rrnB_T2_terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        7928..7956
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            8125..8409
                     /label="Ampicillin (576 - 860)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      join(8468..8668,1..99)
                     /label="f1_origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
       61 gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc
      121 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
      181 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa
      241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
      301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      481 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtcaggcatt tgagaagcac
      601 acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct
      661 atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat
      721 tcatccgctt attatcactt attcaggcgt agcaccaggc gtttaagggc accaataact
      781 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
      841 cattctgccg acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat
      901 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
      961 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     1021 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1081 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1141 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1201 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     1261 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1321 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1381 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     1441 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     1501 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
     1561 tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac caggatttat
     1621 ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga
     1681 tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg
     1741 tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca
     1801 ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg
     1861 tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat
     1921 atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     1981 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
     2041 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
     2101 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     2161 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     2221 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     2281 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     2341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
     2401 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
     2461 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
     2521 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
     2581 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
     2641 taatcagata aaaTATTTCT AGGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT
     2701 CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA
     2761 CGATGCGTCC GGCGTAGAGG ATCTGCTCAT GTTTGACAGC TTATCATCGa tttgacagct
     2821 agctcagtcc taggtataat actagtATAG GTTAATGTCA ATTCAAgttt tagagctaga
     2881 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2941 gctttttttg aagctCTGAC AGCTAGCTCA GTCCTAGGTA TAATGCTAGc CCCGGGAAAA
     3001 GATCTAAAGA GGAGAAAGGA TCTATGACAG AAGACCCGCA ACATCTCACC CGCTCCGAGC
     3061 GCTTCCGCTC GGCCATCGCC GCGTTCATCG ACGCGCGGCG CGAGGCCAAG CTGAAGGGCA
     3121 ATGAGGGCGA TAGCGACACA GATTCCAAGT ACGACTACGC CACCTGGCTG TCGGACGCGG
     3181 CCCGCCGGGT CAGCCAGATC CAGGCCGTGA CCCATGTGCT CAAGGCCACA CACCCCGATG
     3241 CGCGCGGCAG CAGCCTACAC GTCGCCCCCA CCCGTTTGCA GGCGCATACG GAAGTCGGCA
     3301 CCCACGTGCT CGGCACGGAC TATGCCGAAG ACGTGGTCGG CAACGCCGCG GCGCTGGACG
     3361 TGTTCAAACT GCTCAAGCTT GAGGTCGACG GTCGGCGCCT GCTCGACTGG ATGCAGGACG
     3421 ACGACGCGGA TCTGCGCGTG GCGCTGCATG ACAATGCGAA GGTCGCCTCG GACTGGATGG
     3481 GGGCATTCTG CAATCTGGTC CGTCACGATG CCCTACCCAG TTCGCACCAG GCCGCCAAGC
     3541 AAGTGTACTG GCTAGTCGGC GACGAGCCGA GAGAGGATAC CCACTACCAC CTGCTGCAAC
     3601 CGCTGTTTTC CAGCAGTCTG GCCCACGCAG TGCATGCGGA TATTCAGGAC GCGCGTTTCG
     3661 GGGAACGCAA CAAACAGGCG CGACAGGCAT ATCGCGACAA GCACCCATTC GACGGCACCT
     3721 ACCACGACTA CCGCAATCTG GTCGCACGCA AACTGGGCGG CACCAAGCCG CAGAACATCA
     3781 GCCAGCTCAA TAGCGAGCGC GGCGGCATCA ACTACCTGCT GGCCTCGCTG CCACCGCGCT
     3841 GGACGCAGGA AGAGCGTCCT CGCACGTTGC TCAAGCTGGA CTCGGCGCTG GATCGCTTCG
     3901 CCTATTTCGA TGAAGTGCCG ACGCTGATCA AGAAGCTGGC GGATTTCCTG GCCACCGATC
     3961 CCCCAAAGAA CGATGCAACT CAGAAAAAAC GCGAGGGCAT GGAAAACGAC TTGATCAACA
     4021 AGCTGGCGCT GTTTGCCGCC GAGACCGCAG TGCGTTTCGA GCCAGGCTGG AGCCGCAACC
     4081 CGGACTGCGT ATTGCCACTC TCGCAGCAAC TCTGGCTGGA CGCCGAACGC GTGGAACTGC
     4141 CAATCCGCAT CGATCCGGAG CATGCAGAGT GGGAACAGCA AGATCGCAAC TTCGTGGACA
     4201 CTTACCATCT CGGTGATTGG CAGGACGAAA TCGCCGGACA ATTCGCCACT TGGCTCAATG
     4261 ACCGACTACG CGCCGCCGGG ATCACCGGCC TCGGTGACGA CCAGTACCGG CATTGGGCCA
     4321 AGCAAGCCAT CGTCGATGCG GCTTGGCCCG TGCCGATGCG CCGACGCGCA CCGGCCGGAG
     4381 GTGTGACATG AGCAGTTGTC CCCCTTTCGA CCACTTGCTG GTGGTGCCGC ACCTGCGCGT
     4441 GCAGAACGCC AATGCCGTTT CCAGCCCGCT CACCCACGGC TTTCCCTCGA TCACCGCGTT
     4501 CCTGGGCCTG ATGTGGGCGC TGGAACGCAA GGCGCGTATC GCCGGACTGG ATCTGGCCTT
     4561 CAACGCAGTC GGCGTGGTCT GCCACGACCA TCAAGAACAG GTCACCCAAG GGAACTTCGT
     4621 CAAGAGCTTC TGTCTGACCC GCAATCCGGT AAACAAAGAC GGTGCAACCG CGGCGATTGT
     4681 TGAGGAAGGC CGCATTCACT TGGAACTGAG CCTGGTATTC GCGGTGCACA GCATGCGCTG
     4741 GCTAGGCGAT CCGGCCACTC ACGTCGCGGA TCTGGCCGTG GTGACCAAGC TGCTGGCCGG
     4801 CATGCGCGTC GCTGGCGGCA GCGTGCTGCC GTCCACGCAC GGCTGGCGTC CACGCCATCA
     4861 AGCGCAGGTC ATCGCCTGCA CTGGCACGCC GTCCGACCAG CAGGCGTTGT TCCGCAAACT
     4921 ACGACTGCGG CTGTTGCCCG GCTTCGCCCT GGTGGCACGC GACGACCTGC TCGGCACGCG
     4981 GCTGGCCGAA CTGCAAGAAC ACACGCCCGA GGCCACCGGC CTGCACGCTT GGCTTTCGCT
     5041 GTCGCGCATC AACTGGCGTC ACGTGCCGGA TGCAGCGGCT GGCAAAGGCG AGTGGCAACA
     5101 CGATCGCAGC GGCCGGGGCT GGATCGTGCC CATCCCGGTG GGCTACGGCG CGCTCGGAGC
     5161 CGTGCACAAG GCCGGCAGCG TGGCCAATGC ACGCGATGCC AACACCCCGT TCCGCTTCGT
     5221 CGAGAGCCTC TACTCGGTCG GCGAATGGAT CAGCCCGCAC CGCCTGCAAA CGCCGCATCA
     5281 CCTGCTCTGG TACGCCGACA GCCAACCCGC TGCCGACCTG TACCGCTGCC GTAACGATTA
     5341 TCGCCCCGCC ACCACCCACC ACTTCGATTG ACCACACGCA CGACAAGGAA TTCCATCATG
     5401 GCTCACGATA CCCTCAAGAC CGCATCCGTC CTCGCCTTCG AGCGCAAACT CGATCCGTCC
     5461 GACGCGCTGT TCTTCTCCGG CAGTTGGTCC GCACGCCATA CGGCGCGCGA TTGGCAGCCG
     5521 GTGCGCATCC GCGAAAAGTC GGTGCGCGGC ACCATTTCCA ACCGGCTCAA GGACAAGAGC
     5581 AAGGAACAAT CCGACCCGGC CAAACTCGAC GCCGCCATCC AGAACCCCAA CCTGCAAACC
     5641 GTGGACGTCG CCGCACTCCC GCTGGAGGCG GACACGCTGA AAGTGCAGTT CACCCTACGC
     5701 GTGCTTGACG GCACTGGCGA ACCTTCGGCC TGCAACGATA CCGACTATCG CAAGAAACTC
     5761 CTCGGCACCG TCAGTGATTA CTTGCAGCAG CATGGCTTCA GCGAGCTGGC ACGGCGCTAC
     5821 GCGGCGAATC TCGCCAATGG TCGCTTCCTC TGGCGCAACC GCATCGGTGC AGAACAGGTG
     5881 GAGGTGCATG TCGCGCACCT GCGCGACGGT AACCCAGTGA CGCAGTGGAC CTTCCAGGCG
     5941 CTTGACCATG CCCTACGCAC GCTGTCCGCA CCCAGCGACG CAGCCGAGAG CCTGAACGCC
     6001 TTGGCCGCGA CCATCGCCGA CGGCCTGACC GGCAAAACAC ATGTGCTGCT GCAAGTCACC
     6061 GCCTTCGTCC GCATCGGTGC CGGTCAGGAA GTGTTCCCCT CGCAGGAACT GATCCTCGAC
     6121 CGCGGCCGCG GCGACAAAAG CAAGACGCTC TACGCGGTCG GCGAAGGCGA CAAAGCCGTG
     6181 GCGGCGATTC ACTCGCAGAA GATCGGCAAT GCACTGCGCA CCATCGACAC GTGGTATCCG
     6241 GGCGTCGAGG AAAACGGCGC GATTGCCGTC GAACCCTATG GCTCGGTCAC CACGCAAGGC
     6301 AAAGCGTATC GACAACCGAA GGACAAGCAG GACTTCTACA ACCTGCTTGA TGCGTGGGTG
     6361 ATCAAGGACA AAGTGCCGAG CGTGGAGCAA CAGCATTTCG TCATCGCCAC GTTGATTCGC
     6421 GGCGGTGTGT TCGGCGAATC GAACTGAGGC AAGGCTATGC AGCATTATCT CGATCTGCAT
     6481 CTTCGCCCCG ACCCGGAGCT TGCGCCCTAT CAACTGCTTG GCGCGCTGTA CGCGCGCCTG
     6541 CACCGGTCCC TGGTGACATT GAACACCACC AGGATCGGCG TGAGCTTTCC CGGCCATGAC
     6601 AACCGCGTAC CGACTCTAGG GACGCATCTG CGGCTGCATG GTGACGACAG CACCCTGCAC
     6661 CATCTGATGG CAACCACCTG GCTGCATGGT GTTCGCGACC ATGTGACCAT CACCAGCATC
     6721 GGTGCCGTGC CATCCGAGGC AGTGCACCGA CAAGTGACGC GTGTGCAGGC CAAAAGCAGC
     6781 CCTGAACGCC TGCGTCGGCG TGCCATGCGC CGACATGGGA TAAGTGAGGA TCTCGCCGTC
     6841 CAACGCATTC CAGATTCGGC GGCAGAACAG TTGCGACTTC CGTTCGTCGT ACTGGGCAGC
     6901 CGCAGCACCG GCCAAACCGC GTTCCCGGTC TTCGTACGGC ATGGCCCCGT ACAGCAAGAA
     6961 CCCGTACCTG GCGACTTCAG CAGTTATGGA CTCAGCCGGG GAGCCACCGT TCCCTGGTTC
     7021 TGAtaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
     7081 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     7141 caccttcggg tggGCCTTTC TGCGTTTATA CCTAGGGAGA CGTCcaaata ggggttccgc
     7201 gcacgcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
     7261 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     7321 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     7381 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     7441 tGGGCCTTTC GTTTTATCTG Tctgcaggca tgcaagcttg gctgttttgg cggatgagag
     7501 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
     7561 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
     7621 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
     7681 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     7741 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     7801 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca
     7861 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct
     7921 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7981 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8041 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8101 aagatcagtt ggggcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8161 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8221 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8281 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8341 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8401 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt acgcgccctg
     8461 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8521 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8581 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8641 gcacctcgac cccaaaaaac ttgatttg
//