LOCUS       pRoDonor                3203 bp ds-DNA     circular     30-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(192..211)
                     /label="IMo036"
                     /note="sequence: acttactctagcttcccggc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          630..649
                     /label="IMo060"
                     /note="sequence: TCTCAAGGATCTTACCGCTG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          630..649
                     /label="IMo060"
                     /note="sequence: tctcaaggatcttaccgctg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          636..654
                     /label="IMo039"
                     /note="sequence: ggatcttaccgctgttgag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(636..654)
                     /label="IMo038"
                     /note="sequence: ctcaacagcggtaagatcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(799..903)
                     /label="pAmp promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          835..854
                     /label="IMo008"
                     /note="sequence: gggttattgtctcatgagcg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(901..917)
                     /label="IMo201"
                     /note="sequence: ttcggggaaatgtgcgc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          1065..1081
                     /label="IMo198"
                     /note="sequence: agctaagcttAAGTGCCATTCCGCCTG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1070..1087)
                     /label="IMo223"
                     /note="sequence: TTAGGTCAGGCGGAATGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1098..1129
                     /label="Duplicated site"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(1099..1123)
                     /label="IMo221"
                     /note="sequence: TATTATTTAAAGTTTGTTTTTGCCG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1100..1118)
                     /label="End repeat B"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1124..1128
                     /label="Duplication site"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1129..1153
                     /label="IMo221"
                     /note="sequence: TATTATTTAAAGTTTGTTTTTGCCG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1129..1291
                     /label="RTE TXTL seq"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1134..1152
                     /label="Repeat type B"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1157..1175
                     /label="Repeat type B"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1173..1193)
                     /label="IMo229_qPCR- cargo specific through RTE"
                     /note="sequence: GGTTTCAGAGAATCGAGTGGC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1173..1198)
                     /label="IMo238"
                     /note="sequence: ACTAGGGTTTCAGAGAATCGAGTGGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1222..1226)
                     /label="?duplication region"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1234..1252
                     /label="Repeat type B"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1240..1261
                     /label="Repeat type A"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1264..1282
                     /label="Repeat type B"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1270..1291
                     /label="Repeat type A"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1323..1355
                     /label="IMo219"
                     /note="sequence: cGCTCTTCGttgcatggagaaaaaaatcactgg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1332..1355
                     /label="IMo219"
                     /note="sequence: cGCTCTTCGttgcatggagaaaaaaatcactgg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     source          1335..2022
                     /label="source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1335..2117
                     /label="Cy8801_RELE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1336..1995
                     /label="CmR"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     primer_bind     complement(1402..1421)
                     /label="CAT-R"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     primer          complement(1402..1421)
                     /label="IMo007"
                     /note="sequence: gcaactgactgaaatgcctc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(1646..1665)
                     /label="IMo016"
                     /note="sequence: gtggtattcactccagagcg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1697..1717
                     /label="FE-114"
                     /note="sequence: cgcaagatgtggcgtgttacg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1899..1918
                     /label="IMo235"
                     /note="sequence: gattcaggttcatcatgccg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1929..1948)
                     /label="FW0063_pBBR122 CmR rev"
                     /note="sequence: GCATTCTGCCGACATGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1930..1949)
                     /label="FW529 Cm for"
                     /note="sequence: agcattctgccgacatggaa"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1960..1977
                     /label="IMo017"
                     /note="sequence: caacagtactgcgatgag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2006..2022)
                     /label="IMo105"
                     /note="sequence: cGCTCTTCGcaaagggcaccaataactgc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2006..2022)
                     /label="IMo109"
                     /note="sequence: cGCTCTTCGcttagggcaccaataactgc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2006..2022)
                     /label="IMo107"
                     /note="sequence: cGCTCTTCGgagagggcaccaataactgc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2006..2025)
                     /label="IMo109"
                     /note="sequence: cGCTCTTCGcttagggcaccaataactgc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2006..2027
                     /label="IMo228_qPCR- cargo specific through LTE"
                     /note="sequence: gcagttattggtgccctAAGAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2006..2035
                     /label="IMo237"
                     /note="sequence: gcagttattggtgccctAAGACTATTTGAC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2043..2061)
                     /label="Repeat type B"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(2043..2117)
                     /label="LTE TXTL seq"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2043..2117)
                     /label="Left Terminal End"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(2069..2087)
                     /label="Repeat type B"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2088..2117
                     /label="Edge of the 1st TXTL seq result"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2092..2117
                     /label="Duplicated site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(2093..2117)
                     /label="IMo221"
                     /note="sequence: TATTATTTAAAGTTTGTTTTTGCCG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2094..2112)
                     /label="Repeat type A"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2123..2142
                     /label="IMo222"
                     /note="sequence: GGTCATAGCTGTTTCCTGTG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2130..2148)
                     /label="IMo227"
                     /note="sequence: GCTAACTTACATTAATTGCGATTTCACACAGGAAACAGC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2254..2275)
                     /label="prDC309"
                     /note="sequence: ttttgctggccttttgctcaca"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2312..2900)
                     /label="pMB1 ORI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2357..2376)
                     /label="FW0325 seq SpCas9 sgRNA for"
                     /note="sequence: ttcgccacctctgacttgag"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(join(3141..3203,1..798))
                     /label="AmpR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
       61 TGCTGCAATG ATACCGCGAG AGCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
      121 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
      181 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
      241 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
      301 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
      361 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
      421 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
      481 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
      541 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
      601 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
      661 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
      721 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
      781 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
      841 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
      901 GCGCACATTT CCCCGAAAAG TGCCAGATAC CTGAAACAAA ACCCATCGTA CGGCCAAGGA
      961 AGTCTCCAAT AACTGTGATC CACCACAAGC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA
     1021 AACGACGGCC AGTCATGCAT AATCCGCACG CATCTGGAAT AAGGAAGTGC CATTCCGCCT
     1081 GACCTAACAT TAGCTTCGCG GCAAAAACAA ACTTTAAATA ATAGAAAGTA TTATTTAAAG
     1141 TTTGTTTTTG CCGTATTTTA AAGTTTGTTT TTGCCACTCG ATTCTCTGAA ACCCTAGTAA
     1201 AATCGTTGTA CTAAAATTCT AGCATGACCG CTTTTTCAAG TTTGAGTTTG CCGAAATCGT
     1261 TATTTTCAGG TTTGACATTG CCGAAATAGT TGGGACTTGC TATGATGGTT TTATCGTAAA
     1321 TTTGTATTAA TTTGcatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     1381 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     1441 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     1501 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg
     1561 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     1621 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     1681 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     1741 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     1801 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac tatgggcaaa
     1861 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     1921 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     1981 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc ctAAGACTAT TTGACTTAAA
     2041 ATTTTTCAGG CATAGCTTTG CTGTCAGATT TTCAGGCATA ACCTTGCCGA TGCGGCAAAA
     2101 ACAAACTTTA AATAATAGAA AGGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
     2161 AGAGGGCACA ATCCTATTCC GCGCTATCCG ACAATCTCCA AGACATTAGG TGGAGTTCAG
     2221 TTCGGCGTAT GGCATATGTC GCTGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA
     2281 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
     2341 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
     2401 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
     2461 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
     2521 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
     2581 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
     2641 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
     2701 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT
     2761 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
     2821 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     2881 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCTAT
     2941 TCAACAAAGC CGCCGTCCCG TCAAGTCAGC GTAAATGGGT AGGGGGCTTC AAATCGTCCT
     3001 CGTGATACCA ATTCGGAGCC TGCTTTTTTG TACAAACTTG TTGATAATGG CAATTCAAGG
     3061 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     3121 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     3181 TGTCTATTTC GTTCATCCAT AGT
//