LOCUS       Sth_I-E_array_GFP_dropo 2850 bp ds-DNA     circular     09-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(655..759)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(760..1523)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    835..1424
                     /label="pBR322 ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1546..1580
                     /label="J23119"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1581..1608
                     /label="Sth I-E repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1611..1616
                     /label="BbsI recognition"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        1619..1748
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     RBS             1749..1784
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1785..2501
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      2502..2630
                     /label="BBa_B0015 Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2633..2638
                     /label="BbsI recognition"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2641..2668
                     /label="Sth I-E repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2669..2707
                     /label="Terminator"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    complement(2736..2844)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(join(2845..2850,1..654))
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
ORIGIN
        1 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
       61 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      121 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
      181 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
      241 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
      301 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
      361 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
      421 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
      481 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      541 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      601 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      661 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
      721 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
      781 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      841 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      901 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      961 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1021 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1081 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1141 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1201 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1261 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1321 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1381 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1441 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1501 cccctgattc tgtggataac cgtCGAGAGC ATCGATCCTA GCATGttgac agctagctca
     1561 gtcctaggta taatgctagc GTTTTTCCCG CACACGCGGG GGTGATCCgg GTCTTCgtga
     1621 aagtgaaacg tgatttcatg cgtcattttg aacattttgt aaatcttatt taataatgtg
     1681 tgcggcaatt cacatttaat ttatgaatgt tttcttaaca tcgcggcaac tcaagaaacg
     1741 gcaggttcgg atcttagcta ctagagaaag aggagaaata ctagatgcgt aaaggcgaag
     1801 agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc aacggtcata
     1861 agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt
     1921 tcatctgtac tactggtaaa ctgccggttc cttggccgac tctggtaacg acgctgactt
     1981 atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac ttcttcaagt
     2041 ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat gacggcacgt
     2101 acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc attgagctga
     2161 aaggcattga ctttaaagag gacggcaata tcctgggcca taagctggaa tacaatttta
     2221 acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa gcgaatttta
     2281 aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac cagcaaaaca
     2341 ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc acgcaaagcg
     2401 ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag ttcgtaaccg
     2461 cagcgggcat cacgcatggt atggatgaac tgtacaaatg accaggcatc aaataaaacg
     2521 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2581 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata gtGAAGACct
     2641 GTTTTTCCCG CACACGCGGG GGTGATCCaa aaaaaaaccc cgcccctgac agggcggggt
     2701 tttttttAAA GAATCGATCG ATCCCGAATG AGTAGaccaa taaaaaacgc ccggcggcaa
     2761 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     2821 agtccaagcg agctcgatat caaattacgc
//