LOCUS       pSth_Cse2               5803 bp ds-DNA     circular     09-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(618..1206)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1328..2143
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(2236..2691)
                     /label="f1 origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      2728..2775
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(2842..2859)
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2860..3450)
                     /label="Sth Cse2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(2860..3471)
                     /label="Translation 3471-2860"
     protein_bind    3479..3503
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(3504..3522)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        3831..3908
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3909..4991
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             join(5800..5803,1..188)
                     /label="rop"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
ORIGIN
        1 ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
       61 ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
      121 acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa
      181 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      241 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
      301 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
      361 tgtactgaga gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
      421 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      481 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      541 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      601 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      661 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      721 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      781 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      841 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      961 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1021 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     1081 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1141 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1201 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1261 cgttaaggga ttttggtcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     1321 gggtgttatg agccatattc aacgggaaac gtcttgctct aggccgcgat taaattccaa
     1381 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1441 gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     1501 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     1561 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     1621 cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     1681 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     1741 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     1801 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     1861 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     1921 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     1981 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2041 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2101 gaataaattg cagtttcatt tgatgctcga tgagtttttc taagaattaa ttcatgagcg
     2161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2221 gaaaagtgcc acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     2281 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2341 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2401 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2461 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2521 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2581 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2641 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     2701 caatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
     2761 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc
     2821 tttgttagca gccggatctc agtggtggtg gtggtggtgG TTGTCATTAT TCTTTTCACC
     2881 TTTGATCTGT TTGTAATACG CCCGAGCCCA ATTCAATCGT ACGTTTTCCT GAGAATTGTT
     2941 TAAGAACCAG TAAAGATCAT TCGCAAGACC GCTATAATCG ACCTGAATAA CCGGCGATTC
     3001 AACTTTCAGT AAACTTATAA GGTGTCTAAG GTGGTACGTC AGTTCCTCGA AGGTGCTGCT
     3061 TGTGATCATG GTATTAAATC TGCGGTCAAT ACTAGTGGTA TCATCTTCCT TGCGCAGTTT
     3121 ACCCAGGGCG GAACCGATAT TCTCATACGG TTTACTATCA TCCAGCATAA CGCTCGTCGA
     3181 AACACCTTGC TGATGCAATG CATATAACTG TAGGGCTGTA TAAATAGCCA GTTCTTCATA
     3241 ACTAGGTTGC TGATAGCTAC TAAGAAATTC TTCAGGCAGA TTTTCAAACA GTATCGGCCA
     3301 AATTTCAGTC GCTTCACTCA ACGGTTTCCC GAGCGTTTTC CGAACTTTCG CCAGTGTTGC
     3361 TTTGTCATAG GTCTCTTCCT TCTGATTGCT CAGCTTGAAT ATAATTTTTT TAGTGACGGT
     3421 CCATACGGTC TGTTTAGTAT GCTGTGACAT AAAAAATCTG CTTATTCGAG Tctagagggg
     3481 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
     3541 ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     3601 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     3661 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     3721 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     3781 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg
     3841 aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
     3901 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
     3961 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
     4021 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
     4081 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
     4141 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
     4201 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
     4261 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
     4321 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
     4381 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
     4441 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
     4501 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
     4561 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
     4621 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
     4681 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
     4741 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
     4801 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     4861 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
     4921 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4981 gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct
     5041 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     5101 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     5161 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     5221 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     5281 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg
     5341 cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
     5401 tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
     5461 gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
     5521 cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
     5581 ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     5641 ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     5701 gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
     5761 acccccatga acagaaatcc cccttacacg gaggcatcag tga
//