LOCUS       pSth_Cas5               5943 bp ds-DNA     circular     09-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      26..73
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(140..157)
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(158..880)
                     /label="Sth Cas5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(158..880)
                     /label="Translation 880-158"
     protein_bind    917..941
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(942..960)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1269..1346
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1347..2429
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             3238..3429
                     /label="rop"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     rep_origin      complement(3859..4447)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             4569..5384
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(5477..5932)
                     /label="f1 origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgATC GCCTATGGAT TCAAATATAT
      181 TGTGTTCTGT ATTTTTATTC AAATAATGCG GGTTCTGCAC ACTGATTAGT TCTCGGACCT
      241 CGTAGCGGAA CCCGAAGCGA CGACCTTTTT GAGAAAAGGA GCGTACATAG TCTTGCCGTA
      301 ATTGATATAC ATCTTTATTG ACTAACTGAC TATCCAGGTA CACTTCTAGT GAAATGGCAT
      361 CTTTATCGTG GCTTTTTTTC ATGAACCAGA GTGAAGCTTG CCATTTAAGA GATTTCAGGG
      421 ATTCCAGGGG GTTGACATCG GTTTCGCCAA GGATAAAATC AGCCGTAATT GGCAACGCAC
      481 GGCGACCCAT GAACGGTTGG AAATACGGGT TTTTGAGTGC TCCTTTAATA ATATCAATGA
      541 AACTGTCGTC GGTATGGGAG AGCGCCACTA CAAACACAGC GTCTTCTAGA TAATAACGAT
      601 TTGTAACATA ATTCCGATCG AATTTACCTT TTTCAGTATA TTTCTGGGCC ACATGAAAGT
      661 CTCGAAGCAA GTTCCCCTGC TGATCTACAC GGACCGCAAA ATCGAGCTCA TTAAGTTTTT
      721 GTATCTTCTC GTCCTCATCC CGTCTATACC CTAAACTTGC AGCCAGGAGA CCGATGACTG
      781 CGCTTTTAGA CGGGTAAAAG TCGGTGTGCC TGGTTTCAAA ATGTGAGCTG GTCCCCCAAC
      841 TTTGCAGAGG ACCTGCAAAC TTTAACAGGA TTGTTTTCAT AAATTACCTC CTTATTGGAC
      901 TAATTTAGGc tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta
      961 atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg
     1021 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     1081 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     1141 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     1201 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     1261 agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg
     1321 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
     1381 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
     1441 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
     1501 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
     1561 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
     1621 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
     1681 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
     1741 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
     1801 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
     1861 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
     1921 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
     1981 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
     2041 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
     2101 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
     2161 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
     2221 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     2281 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     2341 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2401 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact
     2461 cattaggcac cgggatctcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     2521 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     2581 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     2641 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     2701 gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     2761 atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     2821 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     2881 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     2941 tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
     3001 gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
     3061 tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
     3121 caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     3181 cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     3241 accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     3301 cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt
     3361 cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa
     3421 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3481 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     3541 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     3601 ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     3661 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3721 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3901 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3961 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4021 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4081 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4141 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4201 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4261 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4321 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4381 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4441 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4501 acgttaaggg attttggtca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     4561 ggggtgttat gagccatatt caacgggaaa cgtcttgctc taggccgcga ttaaattcca
     4621 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     4681 cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     4741 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     4801 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     4861 ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     4921 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     4981 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     5041 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     5101 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     5161 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     5221 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     5281 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     5341 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagaatta attcatgagc
     5401 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5461 cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     5521 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     5581 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     5641 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     5701 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     5761 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     5821 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     5881 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     5941 cca
//