LOCUS       pSth_Cas6               5854 bp ds-DNA     circular     09-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(590..1178)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1300..2115
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(2208..2663)
                     /label="f1 origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      2700..2747
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(2814..2831)
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2832..3467)
                     /label="Sth Cas6"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(2832..3467)
                     /label="Translation 3467-2832"
     protein_bind    3502..3526
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(3527..3545)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        3854..3931
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3932..5014
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             join(5823..5854,1..160)
                     /label="rop"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
ORIGIN
        1 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
       61 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
      121 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
      181 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      241 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
      301 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt
      361 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
      421 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      481 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      541 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      601 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      661 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      721 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      781 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      841 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      901 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      961 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1021 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1081 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1141 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1201 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgaacaata
     1261 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1321 acgtcttgct ctaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1381 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
     1441 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1501 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1561 atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc
     1621 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1681 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1741 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1801 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1861 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1921 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1981 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2041 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2101 gatgagtttt tctaagaatt aattcatgag cggatacata tttgaatgta tttagaaaaa
     2161 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt
     2221 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     2281 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     2341 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     2401 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     2461 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     2521 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     2581 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     2641 taatgcgccg ctacagggcg cgtcccattc gccaatccgg atatagttcc tcctttcagc
     2701 aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg
     2761 gtggcagcag ccaactcagc ttcctttcgg gctttgttag cagccggatc tcagtggtgg
     2821 tggtggtggt gTTCTTCAAG TGGCACGACC GTCATCAAGC CGAATCCATA AGCTTTTTTT
     2881 TTACCCACGC CCTCCGTCAA CATTTTTTTA AAGAGCGTGT CGTCCGAAAC CGTAAGGGTT
     2941 CCTTCATACA CGACCTTGCT CAGGCGTATC GGTTTTTCTG GCTTTTGAAA GACTTCATAA
     3001 CCTCGTTCCA CAATGCTGAA ACTTTCTCCA ACTAATGAGA ACCCATTTTT TTCAGATTGT
     3061 TTTACCAGAA AGTCCATCTG GTGTTTGATT GTCACATGCG GTTTAACTAC CCCGCGCTTA
     3121 GATTTATCAG GAGAGGTGAT ACTAATTACC GGGTTAAGCA CGATGCGAAA ATTCATACGG
     3181 AAACCGGTTT TGATAGAATC GAGAAAGTGC TGATAATCTT TAGTTTGTGC ACTACCAACC
     3241 ACGCCGTATT TCTCGAGTTT CTGCAGGTTC GGTTTTTCTT TGCTCACAAT GATCAAATAA
     3301 TCCTTGCCCT GGATACGGTC AATGCGCCAG AGCTTTCGCG TGCGAATGCT TTGTTCGAGC
     3361 TCTGACGGAA AAGACTCTTC GACCCAAGCG TGGTATGCAC CAACATGTGT CAGATCCCGC
     3421 ACCTTTCGGC GATTATACCG ATCAATTTCC ACGCGAGATA TATACATCTA AAAAACCTCC
     3481 TTACCTTGTT TACCctagag gggaattgtt atccgctcac aattccccta tagtgagtcg
     3541 tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
     3601 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     3661 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     3721 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     3781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     3841 cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg
     3901 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
     3961 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
     4021 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
     4081 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
     4141 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
     4201 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
     4261 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
     4321 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
     4381 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
     4441 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
     4501 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
     4561 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
     4621 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
     4681 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
     4741 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
     4801 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     4861 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
     4921 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4981 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc
     5041 tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct
     5101 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     5161 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     5221 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     5281 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     5341 ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
     5401 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
     5461 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
     5521 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct
     5581 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
     5641 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac
     5701 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc
     5761 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat
     5821 cagtgaccaa acaggaaaaa accgccctta acat
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