LOCUS       pSth_Cas7               6288 bp ds-DNA     circular     09-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      26..73
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(140..157)
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(158..1222)
                     /label="Sth Cas7"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(158..1222)
                     /label="Translation 1222-158"
     protein_bind    1262..1286
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(1287..1305)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1614..1691
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1692..2774
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             3583..3774
                     /label="rop"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     rep_origin      complement(4204..4792)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             4914..5729
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(5822..6277)
                     /label="f1 origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgAAT GTACTGTGCG ATCATTTCGG
      181 AAAAATCCTC CAGTAACTTC GAGACAGAAT CCTTTTCCTC ACCGATCTGC TTCAGAGAAT
      241 CAGATTCATG TAGGGTAACG TAAAAGGTCA TTAATGGTTT ATCTACGAAT TTTTCCACTT
      301 TTATAAACTC TTTAGCTAGT TTGTCTATGC TTTTAGTGAC ATAGCCTTCG ATGCTTTTTA
      361 CAGGTTCTTC AAAGGCGGAG ACCAAATTGA CCGGACGGTC GGAGCGGACG GTAACAATCA
      421 AAGTTTGCGG TAAGGTCTGA TTTGCAAATG TGTTAACCTT CCCAGTTGGA AGGCTCTTAG
      481 CGAAGGCTTC GATAAACAGC GACAGACTGT TTTCCAAACT CTCCTTATTC CCCAGCTGTT
      541 TCGATAGCTC ATGAATCGCC ACATTGGCAT AACGGTAAAG TGTAGAAGAG TTAAACTCAA
      601 CGGTGCCCAG CATGCCGGCG CCCGCATTAT CTTCAGGGGC TAAGTCGTCT ACCGCAGTGA
      661 AGAAATCGAA TTCCGTCTGC ACTGGATGTG TAGAAATAGC ATGGGCCACT TGACTCGACG
      721 AGTCCTCATT AAGTGTCGGA TCTTCAGCGA CCATTCGGCC AAAAAGGGCA ATGTCGATAG
      781 CTGGATCTTC ATTGGCCAAT TTCTGTAATT CCTTTTTGTG CGTTATGTTA TCATACGCAG
      841 CCTGGGCAAG TTTTTTGGCC TGAGAATCCC CCATAAAGTA AAGTGCTTTA ACTTCGCCTT
      901 TTTTTTTGAT CCCCGCAGCG ATCAGAACTT TATCGGCTTT TTCTAATGCA TCCTCTATGC
      961 TTATGGAGGA ATCAAGTTCT ACGATTTTAT CCGCTACATA ACGCACGATT TCTTTCGTAC
     1021 GTATTCCGAC ATTACTATGG TCGCCGTGGG TATTAAAATA TTCGCGCATG GCTTTTTTCC
     1081 ACGCCTGTGA CGATACACGG CTTCGACGAA CACCACCATA CTGGGCGGTT TTCGGACTAC
     1141 CGGTATCATC CCGATTCATA TTTGACGGTG GTAAGGACTG AATGGCGTGA ATGTCCAGAA
     1201 AAAGCCTCTG CTCGGTGGTC ATAATAACCT TCCTTAATTT GTAGTGTGTA ATGTctagag
     1261 gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga
     1321 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     1381 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     1441 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     1501 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     1561 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca
     1621 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
     1681 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     1741 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     1801 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     1861 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     1921 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     1981 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     2041 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     2101 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     2161 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     2221 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     2281 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     2341 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     2401 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     2461 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     2521 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     2581 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     2641 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     2701 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     2761 aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga
     2821 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2881 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2941 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     3001 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     3061 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccccacgg
     3121 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
     3181 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
     3241 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
     3301 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
     3361 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
     3421 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
     3481 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     3541 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa
     3601 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc
     3661 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat
     3721 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     3781 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     3841 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     3901 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     3961 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     4021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4621 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4861 ggtcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     4921 atattcaacg ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg gatgctgatt
     4981 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat
     5041 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     5101 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     5161 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     5221 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     5281 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     5341 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     5401 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     5461 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     5521 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     5581 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     5641 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     5701 ttcatttgat gctcgatgag tttttctaag aattaattca tgagcggata catatttgaa
     5761 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5821 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     5881 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     5941 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     6001 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     6061 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     6121 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     6181 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     6241 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca
//