LOCUS       pATetO_6XHis-Taq_DNA_Pol        5344 bp    DNA     circular UNA 03-NOV-2021
DEFINITION  tet-inducible system with N-term periplasmic tag and C-term strep
            tag.
ACCESSION   urn.local...d-e1zgwtg
VERSION     urn.local...d-e1zgwtg
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(138..761)
                     /note="Geneious type: ORF"
                     /standard_name="tet repressor"
     misc_feature    complement(771..1631)
                     /note="Geneious type: ORF"
                     /standard_name="AmpR"
     promoter        complement(1632..1715)
     terminator      1747..1837
     promoter        1881..1946
     misc_feature    1970..1979
                     /standard_name="RBS"
     sig_peptide     <1991..2023
                     /standard_name="6X His tag wATG"
     primer_bind     complement(2018..2035)
                     /standard_name="Left flank"
     misc_feature    2024..2035
                     /note="Geneious type: polylinker"
                     /standard_name="Factor Xa site"
     misc_feature    2036..4531
                     /note="Geneious type: ORF"
                     /standard_name="Taq DNA Pol"
     primer_bind     4532..4561
                     /standard_name="Right flank"
     terminator      4578..4659
     rep_origin      complement(4741..5328)
ORIGIN      
        1 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
       61 agattatcaa aaaggatctt cacctagatc cttttaaact agtgaagtta ccatcacgga
      121 aaaaggttat gctgctttta agacccactt tcacatttaa gttgtttttc taatccgcat
      181 atgatcaatt caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg
      241 atagcttgtc gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta
      301 gcgacttgat gctcttgatc ttccaatacg caacctaaag taaaatgccc cactgcgctg
      361 agtgcatata atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt
      421 tcgagagttt catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg
      481 cgatgactta gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag
      541 ctttcccctt ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag
      601 gcgtcgagca aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct
      661 agcttctggg cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct
      721 aatgcgctgt taatcacttt acttttatct aaacgagaca tcattaattc ctaccaatgc
      781 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      841 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      901 atgataccgc gagagccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      961 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctatcaat
     1021 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1081 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1141 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1201 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1261 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1321 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1381 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1441 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1501 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1561 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1621 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1681 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1741 tttcccggga actgccagac atcaaataaa acaaaaggct cagtcggaag actgggcctt
     1801 ttgttttatc tgttgtttgt cggtgaacac tctcccgatt gcgatacaga ccctaatttc
     1861 acatcatatg acactaatcc taatttttgt tgacactcta tcattgatag agttatttta
     1921 cctctcccta tcagtgatag agaaaactag aaataatttt gtttaacttt aagaaggaga
     1981 tatacatatg gctagcagag gatcgcatca ccatcaccat cacatcgaag ggcgcatgcg
     2041 tggtatgctg ccgctgttcg aaccgaaagg tcgtgttctg ctggttgacg gtcaccacct
     2101 ggcgtaccgt accttccacg cgctgaaagg tctgaccacc tctcgtggtg aaccggttca
     2161 ggcggtttac ggtttcgcga aatctctgct gaaagcgctg aaagaagacg gtgacgcggt
     2221 tatcgttgtt ttcgacgcga aagcgccgtc tttccgtcac gaagcgtacg gtggttacaa
     2281 agcgggtcgt gcgccgaccc cggaagactt cccgcgtcag ctggcgctga tcaaagaact
     2341 ggttgacctg ctgggtctgg cgcgtctgga agttccgggt tacgaagcgg acgacgttct
     2401 ggcgtctctg gcgaaaaaag cggaaaaaga aggttacgaa gttcgtatcc tgaccgcgga
     2461 caaagacctg taccagctgc tgtctgaccg tatccacgcg ctgcacccgg aaggttacct
     2521 gatcaccccg gcgtggctgt gggaaaaata cggtctgcgt ccggaccagt gggcggacta
     2581 ccgtgcgctg accggtgacg aatctgacaa cctgccgggt gttaaaggta tcggtgaaaa
     2641 aaccgcgcgt aaactgctgg aagaatgggg ttctctggaa gcgctgctga aaaacctgga
     2701 ccgtctgaaa ccggcgatcc gtgaaaaaat cctggcgcac atggacgacc tgaaactgtc
     2761 ttgggacctg gcgaaagttc gtaccgacct gccgctggaa gttgacttcg cgaaacgtcg
     2821 tgaaccggac cgtgaacgtc tgcgtgcgtt cctggaacgt ctggaattcg gttctctgct
     2881 gcacgaattc ggtctgctgg aatctccgaa agcgctggaa gaagcgccgt ggccgccgcc
     2941 ggaaggtgcg ttcgttggtt tcgttctgtc tcgtaaagaa ccgatgtggg cggacctgct
     3001 ggcgctggcg gcggcgcgtg gtggtcgtgt tcaccgtgcg ccggaaccgt acaaagcgct
     3061 gcgtgacctg aaagaagcgc gtggtctgct ggcgaaagac ctgtctgttc tggcgctgcg
     3121 tgaaggtctg ggtctgccgc cgggtgacga cccgatgctg ctggcgtacc tgctggaccc
     3181 gtctaacacc accccggaag gtgttgcgcg tcgttacggt ggtgaatgga ccgaagaagc
     3241 gggtgaacgt gcggcgctgt ctgaacgtct gttcgcgaac ctgtggggtc gtctggaagg
     3301 tgaagaacgt ctgctgtggc tgtaccgtga agttgaacgt ccgctgtctg cggttctggc
     3361 gcacatggaa gcgaccggtg ttcgtctgga cgttgcgtac ctgcgtgcgc tgtctctgga
     3421 agttgcggaa gaaatcgcgc gtctggaagc ggaagttttc cgtctggcgg gtcacccgtt
     3481 caacctgaac tctcgtgacc aactggaacg tgttctgttc gacgaactgg gtctgccggc
     3541 gatcggtaaa accgaaaaaa ccggtaaacg ttctacctct gcggcggttc tggaagcgct
     3601 gcgtgaagcg cacccgatcg ttgaaaaaat cctgcagtac cgtgaactga ccaaactgaa
     3661 atctacctac atcgacccgc tgccggacct gatccacccg cgtaccggtc gtctgcacac
     3721 ccgtttcaac cagaccgcga ccgcgaccgg tcgtctgtct tcttctgacc cgaacctgca
     3781 gaacatcccg gttcgtaccc cactgggtca gcgtatccgt cgtgcgttca tcgcggaaga
     3841 aggttggctt ctggttgcgc tggactactc tcagatcgaa ctgcgtgttc tggcgcacct
     3901 gtctggtgac gaaaacctga tccgtgtttt ccaggaaggt cgtgacatcc acaccgaaac
     3961 cgcgtcttgg atgttcggtg ttccgcgtga agcggttgac ccgctgatgc gtcgtgcggc
     4021 gaaaaccatc aacttcggtg ttctgtacgg tatgtctgcg caccgtctgt ctcaggaact
     4081 ggcgatcccg tacgaagaag cgcaggcgtt catcgaacgt tacttccagt ctttcccgaa
     4141 agttcgtgcg tggatcgaaa aaaccctgga agaaggtcgt cgtcgtggtt acgttgaaac
     4201 cctgttcggt cgtcgtcgtt acgttccgga cctggaagcg cgtgttaaat ctgttcgtga
     4261 agcggcggaa cgtatggcgt tcaacatgcc ggttcagggt accgcggcgg acctgatgaa
     4321 actggcgatg gttaaactgt tcccgcgtct ggaagaaatg ggtgcgcgta tgctgctgca
     4381 ggttcacgac gaactggttc tggaagcgcc gaaagaacgt gcggaagcgg ttgcgcgtct
     4441 ggcgaaagaa gttatggaag gtgtttaccc gctggcggtt ccgctggaag ttgaagttgg
     4501 tatcggtgaa gactggctgt ctgcgaaaga atgataattg cctctgccaa aattctgtcc
     4561 tcaagcgttt tagttcgtcg gtcagtttca cctgatttac gtaaaaaccc gcttcggcgg
     4621 gtttttgctt ttggaggggc agaaagatga atgactgtcg gccattcgat ggtgtcgggt
     4681 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4921 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5161 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5341 gatc
//
