LOCUS       pFastBacMam6_3C_        6589 bp    DNA     circular     01-Nov-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     rep_origin      6132..6588
                     /label="rep_origin f1 ori"
                     /note="f1 ori"
     rep_origin      4323..4996
                     /label="rep_origin pUC ori"
                     /note="pUC ori"
     CDS             complement(5144..5803)
                     /label="CDS AmpR"
                     /note="AmpR"
     misc_recomb     3855..4079
                     /label="misc_recomb Tn7R"
                     /note="Tn7R"
     CDS             3255..3788
                     /label="CDS GenR"
                     /note="GenR"
     promoter        2559..2686
                     /label="promoter Polyhedrin promoter"
                     /note="Polyhedrin promoter"
     polyA_signal     377..617
                     /label="polyA_signal SV40 PolyA signal"
                     /note="SV40 PolyA signal"
     promoter        701..1253
                     /label="promoter CMV_immearly_promoter"
                     /note="CMV_immearly_promoter"
     primer_bind     1256..1280
                     /label="primer_bind LNCX_primer"
                     /note="LNCX_primer"
     primer_bind     2351..2370
                     /label="primer_bind EBV_rev_primer"
                     /note="EBV_rev_primer"
     misc_recomb     183..348
                     /label="misc_recomb Tn7L"
                     /note="Tn7L"
     misc_feature     756..1043
                     /label="misc_feature CAG_enhancer"
                     /note="CAG_enhancer"
     intron          1395..1527
                     /label="intron chim_intron"
                     /note="chim_intron"
     CDS             1644..1667
                     /label="gene 3C protease site"
                     /note="3C protease site"
     primer_bind     2615..2639
                     /label="primer_bind pBlueBac Primer 1"
                     /note="pBlueBac Primer 1"
     promoter        1199..1280
                     /label="promoter CMV_promoter"
                     /note="CMV_promoter"
     promoter        1572..1590
                     /label="promoter T7_promoter"
                     /note="T7_promoter"
     gene            1713..1739
                     /label="gene 8His-stop"
                     /note="8His-stop"
     primer_bind     536..557
                     /label="primer_bind pFastBac primer R1"
                     /note="pFastBac primer R1"
     primer_bind     1210..1230
                     /label="primer_bind CMV_fwd_primer"
                     /note="CMV_fwd_primer"
     misc_feature     1742..2317
                     /label="misc_feature WPRE"
                     /note="WPRE"
     gene            1668..1712
                     /label="gene GAAEDQVDPRLIDGK"
                     /note="GAAEDQVDPRLIDGK"
     primer_bind     417..438
                     /label="primer_bind pBlueBac primer 2"
                     /note="pBlueBac primer 2"
     primer_bind     1254..1273
                     /label="primer_bind pCEP_fwd_primer"
                     /note="pCEP_fwd_primer"
     terminator      2337..2456
                     /label="terminator SV40_PA_terminator"
                     /note="SV40_PA_terminator"
     primer_bind     1515..1538
                     /label="primer_bind BacMam seq1 primer"
                     /note="BacMam seq1 primer"
     primer_bind     1813..1836
                     /label="primer_bind BacMam seq2 primer"
                     /note="BacMam seq2 primer"
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa 
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg 
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc 
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt 
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa 
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag 
      361 gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac 
      421 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 
      481 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 
      541 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 
      601 tatggctgat tatgatcctc tagtacttct cgacaagctt ggtaccgcat gcctcgagac 
      661 tgcaggctct agattcgaaa gcggccgcga ctagttatta atagtaatca attacggggt 
      721 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 
      781 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 
      841 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 
      901 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg 
      961 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc 
     1021 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca 
     1081 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 
     1141 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg 
     1201 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 
     1261 gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg 
     1321 ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt 
     1381 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac 
     1441 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg 
     1501 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag 
     1561 gctagagtac ttaatacgac tcactatagg ctagcctcga gaattcacgc gtggtacctc 
     1621 tagagtcgac ccgggcggcc gcattggaag ttctgtttca agggcctggg gccgcagaag 
     1681 atcaagttga tccaagattg attgatggca agcatcacca tcaccatcac catcactaga 
     1741 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 
     1801 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 
     1861 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 
     1921 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 
     1981 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 
     2041 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 
     2101 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 
     2161 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 
     2221 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 
     2281 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagacatgat 
     2341 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat 
     2401 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt 
     2461 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt 
     2521 ttaaagcaag taaaacctct acaaatgtgg taggatccgc gcccgatggt gggacggtat 
     2581 gaataatccg gaatatttat aggttttttt attacaaaac tgttacgaaa acagtaaaat 
     2641 acttatttat ttgcgagatg gttatcattt taattatctc catgatctat taatattccg 
     2701 gagtatacgt agccaaccac tagaactata gctagagtcc tgggcgaaca aacgatgctc 
     2761 gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg 
     2821 ccgcgacggc cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt 
     2881 gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg 
     2941 ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg 
     3001 acgcacaccg tggaaacgga tgaaggcacg aacccagttg acataagcct gttcggttcg 
     3061 taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg 
     3121 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca 
     3181 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt 
     3241 atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta 
     3301 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc 
     3361 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca 
     3421 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat 
     3481 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc 
     3541 tgcccaggtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg 
     3601 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg 
     3661 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc 
     3721 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg 
     3781 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat 
     3841 tgtcacaacg ccgcgaatat agtctttacc atgcccttgg ccacgcccct ctttaatacg 
     3901 acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta 
     3961 tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga 
     4021 ctttattgtc atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacac 
     4081 ccgcttacgc agggcatcca tttattactc aaccgtaacc gattttgcca ggttacgcgg 
     4141 ctggtctgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 
     4201 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     4261 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     4321 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     4381 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     4441 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     4501 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     4561 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     4621 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     4681 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     4741 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     4801 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     4861 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     4921 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     4981 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     5041 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     5101 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     5161 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     5221 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     5281 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     5341 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     5401 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     5461 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     5521 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     5581 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     5641 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     5701 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     5761 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     5821 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     5881 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     5941 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     6001 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     6061 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     6121 aagtgccacc tgaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt 
     6181 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 
     6241 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 
     6301 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 
     6361 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 
     6421 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 
     6481 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 
     6541 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtc
//