LOCUS       pFastBacMam5_Fla        7446 bp    DNA     linear       05-Nov-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     misc_feature     1973..1996
                     /label="EGFP_Int_Rv1 1"
                     /note="EGFP_Int_Rv1"
     misc_feature     1728..1784
                     /label="pFBM4_Flag_Fw  1"
                     /note="pFBM4_Flag_Fw "
     misc_feature     1687..1744
                     /label="pFBM4_Flag_Rv  1"
                     /note="pFBM4_Flag_Rv "
     rep_origin      6989..7445
                     /label="rep_origin f1 ori"
                     /note="f1 ori"
     rep_origin      5180..5853
                     /label="rep_origin pUC ori"
                     /note="pUC ori"
     CDS             complement(6001..6660)
                     /label="CDS AmpR"
                     /note="AmpR"
     misc_recomb     4712..4936
                     /label="misc_recomb Tn7R"
                     /note="Tn7R"
     CDS             4112..4645
                     /label="CDS GenR"
                     /note="GenR"
     promoter        3416..3543
                     /label="promoter Polyhedrin promoter"
                     /note="Polyhedrin promoter"
     polyA_signal     377..617
                     /label="polyA_signal SV40 PolyA signal"
                     /note="SV40 PolyA signal"
     misc_recomb     183..348
                     /label="misc_recomb Tn7L"
                     /note="Tn7L"
     primer_bind     3472..3496
                     /label="primer_bind pBlueBac Primer 1"
                     /note="pBlueBac Primer 1"
     primer_bind     536..557
                     /label="primer_bind pFastBac primer R1"
                     /note="pFastBac primer R1"
     primer_bind     417..438
                     /label="primer_bind pBlueBac primer 2"
                     /note="pBlueBac primer 2"
     gene            2594..2596
                     /label="gene stop"
                     /note="stop"
     promoter        1319..1400
                     /label="promoter CMV_promoter"
                     /note="CMV_promoter"
     primer_bind     3208..3227
                     /label="primer_bind EBV_rev_primer"
                     /note="EBV_rev_primer"
     primer_bind     1376..1400
                     /label="primer_bind LNCX_primer"
                     /note="LNCX_primer"
     promoter        1692..1710
                     /label="promoter T7_promoter"
                     /note="T7_promoter"
     promoter        821..1373
                     /label="promoter CMV_immearly_promoter"
                     /note="CMV_immearly_promoter"
     misc_feature     2599..3174
                     /label="misc_feature WPRE"
                     /note="WPRE"
     misc_feature     876..1163
                     /label="misc_feature CAG_enhancer"
                     /note="CAG_enhancer"
     terminator      3194..3313
                     /label="terminator SV40_PA_terminator"
                     /note="SV40_PA_terminator"
     primer_bind     1330..1350
                     /label="primer_bind CMV_fwd_primer"
                     /note="CMV_fwd_primer"
     CDS             1763..2515
                     /label="gene 8xHis-FGFP(A207K)"
                     /note="8xHis-FGFP(A207K)"
     primer_bind     1374..1393
                     /label="primer_bind pCEP_fwd_primer"
                     /note="pCEP_fwd_primer"
     intron          1515..1647
                     /label="intron chim_intron"
                     /note="chim_intron"
     primer_bind     2448..2470
                     /label="primer_bind EGFP-IntF1 primer"
                     /note="EGFP-IntF1 primer"
     primer_bind     2670..2693
                     /label="primer_bind BacMam seq2 primer"
                     /note="BacMam seq2 primer"
     primer_bind     1635..1658
                     /label="primer_bind BacMam seq1 primer"
                     /note="BacMam seq1 primer"
     gene            2525..2548
                     /label="gene 3C protease site"
                     /note="3C protease site"
     misc_feature     complement(2501..2544)
                     /label="P564"
     misc_feature     2527..2578
                     /label="P565"
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa 
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg 
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc 
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt 
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa 
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag 
      361 gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac 
      421 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 
      481 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 
      541 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 
      601 tatggctgat tatgatcctc tagtacttct cgacaagctt ggtaccccta ggagatcttc 
      661 aatattggcc attagccata ttattcattg gttatatagc ataaatcaat attggctatt 
      721 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa 
      781 tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca attacggggt 
      841 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 
      901 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 
      961 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 
     1021 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg 
     1081 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc 
     1141 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca 
     1201 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 
     1261 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg 
     1321 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 
     1381 gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg 
     1441 ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt 
     1501 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac 
     1561 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg 
     1621 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag 
     1681 gctagagtac ttaatacgac tcactatagg gatcgccggc accatgggtg actacaagga 
     1741 cgacgacgac aagagcagcg gcatgggcag cagccatcat catcatcatc accaccacat 
     1801 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 
     1861 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 
     1921 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 
     1981 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 
     2041 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 
     2101 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 
     2161 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 
     2221 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 
     2281 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 
     2341 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 
     2401 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 
     2461 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagagcag 
     2521 cggcttggaa gttctgtttc aagggccagc tagcgaattc gtcgactcta gactcgaggc 
     2581 atgcgcggcc gcatagaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 
     2641 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 
     2701 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 
     2761 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 
     2821 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 
     2881 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 
     2941 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 
     3001 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 
     3061 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 
     3121 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 
     3181 cccgcctgag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag 
     3241 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 
     3301 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 
     3361 gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtag gatccgcgcc 
     3421 cgatggtggg acggtatgaa taatccggaa tatttatagg tttttttatt acaaaactgt 
     3481 tacgaaaaca gtaaaatact tatttatttg cgagatggtt atcattttaa ttatctccat 
     3541 gatctattaa tattccggag tatacgtagc caaccactag aactatagct agagtcctgg 
     3601 gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc gaaccacttc atccggggtc 
     3661 agcaccaccg gcaagcgccg cgacggccga ggtcttccga tctcctgaag ccagggcaga 
     3721 tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg 
     3781 gagaccgaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg 
     3841 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagttgaca 
     3901 taagcctgtt cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg caactggtcc 
     3961 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 
     4021 gactgttttt ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg 
     4081 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagcaacg atgttacgca 
     4141 gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg 
     4201 taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga 
     4261 gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt 
     4321 gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc 
     4381 tctcgcggct tacgttctgc ccaggtttga gcagccgcgt agtgagatct atatctatga 
     4441 tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct 
     4501 caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga 
     4561 cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga 
     4621 tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct tcccggccgc 
     4681 ggagttgttc ggtaaattgt cacaacgccg cgaatatagt ctttaccatg cccttggcca 
     4741 cgcccctctt taatacgacg ggcaatttgc acttcagaaa atgaagagtt tgctttagcc 
     4801 ataacaaaag tccagtatgc tttttcacag cataactgga ctgatttcag tttacaacta 
     4861 ttctgtctag tttaagactt tattgtcata gtttagatct attttgttca gtttaagact 
     4921 ttattgtccg cccacacccg cttacgcagg gcatccattt attactcaac cgtaaccgat 
     4981 tttgccaggt tacgcggctg gtctgcggtg tgaaataccg cacagatgcg taaggagaaa 
     5041 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     5101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     5161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     5221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     5281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     5341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     5401 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 
     5461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     5521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     5581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     5641 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 
     5701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     5761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     5821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     5881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     5941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     6001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     6061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     6121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     6181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     6241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     6301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     6361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
     6421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
     6481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
     6541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
     6601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
     6661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
     6721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
     6781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
     6841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
     6901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 
     6961 gcgcacattt ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt 
     7021 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 
     7081 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 
     7141 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 
     7201 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 
     7261 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 
     7321 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 
     7381 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 
     7441 cgcgtc
//