LOCUS       pFastBacMam7_Fla        6699 bp    DNA     linear       01-Nov-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     rep_origin      6242..6698
                     /label="rep_origin f1 ori"
                     /note="f1 ori"
     rep_origin      4433..5106
                     /label="rep_origin pUC ori"
                     /note="pUC ori"
     CDS             complement(5254..5913)
                     /label="CDS AmpR"
                     /note="AmpR"
     misc_recomb     3965..4189
                     /label="misc_recomb Tn7R"
                     /note="Tn7R"
     CDS             3365..3898
                     /label="CDS GenR"
                     /note="GenR"
     promoter        2669..2796
                     /label="promoter Polyhedrin promoter"
                     /note="Polyhedrin promoter"
     polyA_signal     377..617
                     /label="polyA_signal SV40 PolyA signal"
                     /note="SV40 PolyA signal"
     misc_recomb     183..348
                     /label="misc_recomb Tn7L"
                     /note="Tn7L"
     primer_bind     2725..2749
                     /label="primer_bind pBlueBac Primer 1"
                     /note="pBlueBac Primer 1"
     primer_bind     536..557
                     /label="primer_bind pFastBac primer R1"
                     /note="pFastBac primer R1"
     primer_bind     417..438
                     /label="primer_bind pBlueBac primer 2"
                     /note="pBlueBac primer 2"
     gene            1847..1849
                     /label="gene stop"
                     /note="stop"
     promoter        1319..1400
                     /label="promoter CMV_promoter"
                     /note="CMV_promoter"
     primer_bind     2461..2480
                     /label="primer_bind EBV_rev_primer"
                     /note="EBV_rev_primer"
     primer_bind     1376..1400
                     /label="primer_bind LNCX_primer"
                     /note="LNCX_primer"
     promoter        821..1373
                     /label="promoter CMV_immearly_promoter"
                     /note="CMV_immearly_promoter"
     misc_feature     1852..2427
                     /label="misc_feature WPRE"
                     /note="WPRE"
     misc_feature     876..1163
                     /label="misc_feature CAG_enhancer"
                     /note="CAG_enhancer"
     terminator      2447..2566
                     /label="terminator SV40_PA_terminator"
                     /note="SV40_PA_terminator"
     primer_bind     1330..1350
                     /label="primer_bind CMV_fwd_primer"
                     /note="CMV_fwd_primer"
     primer_bind     1374..1393
                     /label="primer_bind pCEP_fwd_primer"
                     /note="pCEP_fwd_primer"
     CDS             1775..1795
                     /label="protein_bind TEV protease site"
                     /note="TEV protease site"
     intron          1515..1647
                     /label="intron chim_intron"
                     /note="chim_intron"
     primer_bind     1923..1946
                     /label="primer_bind BacMam seq2 primer"
                     /note="BacMam seq2 primer"
     primer_bind     1635..1658
                     /label="primer_bind BacMam seq1 primer"
                     /note="BacMam seq1 primer"
     CDS             1724..1762
                     /label="FLAG"
     promoter        1692..1710
                     /label="promoter T7_promoter"
                     /note="T7_promoter"
     misc_feature     1766..1800
                     /label="Found -> DNA"
                     /note="P569"
     misc_feature     complement(1750..1781)
                     /label="Found <- DNA"
                     /note="P568"
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa 
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg 
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc 
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt 
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa 
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag 
      361 gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac 
      421 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 
      481 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 
      541 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 
      601 tatggctgat tatgatcctc tagtacttct cgacaagctt ggtaccccta ggagatcttc 
      661 aatattggcc attagccata ttattcattg gttatatagc ataaatcaat attggctatt 
      721 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa 
      781 tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca attacggggt 
      841 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 
      901 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 
      961 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 
     1021 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg 
     1081 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc 
     1141 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca 
     1201 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 
     1261 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg 
     1321 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 
     1381 gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg 
     1441 ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt 
     1501 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac 
     1561 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg 
     1621 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag 
     1681 gctagagtac ttaatacgac tcactatagg gatcgccggc accatgggtg actacaagga 
     1741 cgacgacgac aagagcagcg gcatgggcag cagcgagaac ctgtacttcc agggaggtgg 
     1801 agctagcgaa ttcgtcgact ctagactcga ggcatgcgcg gccgcataga atcaacctct 
     1861 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 
     1921 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 
     1981 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 
     2041 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 
     2101 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 
     2161 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 
     2221 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 
     2281 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 
     2341 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 
     2401 tcagacgagt cggatctccc tttgggccgc ctccccgcct gagacatgat aagatacatt 
     2461 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt 
     2521 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac 
     2581 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag 
     2641 taaaacctct acaaatgtgg taggatccgc gcccgatggt gggacggtat gaataatccg 
     2701 gaatatttat aggttttttt attacaaaac tgttacgaaa acagtaaaat acttatttat 
     2761 ttgcgagatg gttatcattt taattatctc catgatctat taatattccg gagtatacgt 
     2821 agccaaccac tagaactata gctagagtcc tgggcgaaca aacgatgctc gccttccaga 
     2881 aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc 
     2941 cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt gccgtagaag 
     3001 aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc 
     3061 agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg 
     3121 tggaaacgga tgaaggcacg aacccagttg acataagcct gttcggttcg taaactgtaa 
     3181 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt 
     3241 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct 
     3301 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc 
     3361 aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt 
     3421 aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc aagtcaaatc 
     3481 catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca cctactccca 
     3541 acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat tcatcgcgct 
     3601 tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc tgcccaggtt 
     3661 tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg agcaccggag 
     3721 gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg cgcttggtgc 
     3781 ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc tctatacaaa 
     3841 gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg ccacctaaca 
     3901 attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat tgtcacaacg 
     3961 ccgcgaatat agtctttacc atgcccttgg ccacgcccct ctttaatacg acgggcaatt 
     4021 tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta tgctttttca 
     4081 cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga ctttattgtc 
     4141 atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacac ccgcttacgc 
     4201 agggcatcca tttattactc aaccgtaacc gattttgcca ggttacgcgg ctggtctgcg 
     4261 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc 
     4321 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     4381 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     4441 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     4501 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     4561 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     4621 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     4681 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     4741 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     4801 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     4861 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     4921 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     4981 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     5041 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     5101 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     5161 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     5221 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     5281 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     5341 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     5401 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     5461 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     5521 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     5581 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     5641 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     5701 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
     5761 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
     5821 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
     5881 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
     5941 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     6001 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     6061 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     6121 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     6181 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
     6241 tgaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
     6301 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
     6361 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
     6421 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
     6481 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
     6541 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
     6601 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
     6661 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtc
//