LOCUS       pFastBacMam8_Fla        6696 bp    DNA     linear       01-Nov-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     rep_origin      6239..6695
                     /label="rep_origin f1 ori"
                     /note="f1 ori"
     rep_origin      4430..5103
                     /label="rep_origin pUC ori"
                     /note="pUC ori"
     CDS             complement(5251..5910)
                     /label="CDS AmpR"
                     /note="AmpR"
     misc_recomb     3962..4186
                     /label="misc_recomb Tn7R"
                     /note="Tn7R"
     CDS             3362..3895
                     /label="CDS GenR"
                     /note="GenR"
     promoter        2666..2793
                     /label="promoter Polyhedrin promoter"
                     /note="Polyhedrin promoter"
     polyA_signal     377..617
                     /label="polyA_signal SV40 PolyA signal"
                     /note="SV40 PolyA signal"
     misc_recomb     183..348
                     /label="misc_recomb Tn7L"
                     /note="Tn7L"
     primer_bind     2722..2746
                     /label="primer_bind pBlueBac Primer 1"
                     /note="pBlueBac Primer 1"
     primer_bind     536..557
                     /label="primer_bind pFastBac primer R1"
                     /note="pFastBac primer R1"
     primer_bind     417..438
                     /label="primer_bind pBlueBac primer 2"
                     /note="pBlueBac primer 2"
     gene            1844..1846
                     /label="gene stop"
                     /note="stop"
     promoter        1319..1400
                     /label="promoter CMV_promoter"
                     /note="CMV_promoter"
     primer_bind     2458..2477
                     /label="primer_bind EBV_rev_primer"
                     /note="EBV_rev_primer"
     primer_bind     1376..1400
                     /label="primer_bind LNCX_primer"
                     /note="LNCX_primer"
     promoter        1692..1710
                     /label="promoter T7_promoter"
                     /note="T7_promoter"
     promoter        821..1373
                     /label="promoter CMV_immearly_promoter"
                     /note="CMV_immearly_promoter"
     misc_feature     1849..2424
                     /label="misc_feature WPRE"
                     /note="WPRE"
     misc_feature     876..1163
                     /label="misc_feature CAG_enhancer"
                     /note="CAG_enhancer"
     terminator      2444..2563
                     /label="terminator SV40_PA_terminator"
                     /note="SV40_PA_terminator"
     primer_bind     1330..1350
                     /label="primer_bind CMV_fwd_primer"
                     /note="CMV_fwd_primer"
     primer_bind     1374..1393
                     /label="primer_bind pCEP_fwd_primer"
                     /note="pCEP_fwd_primer"
     intron          1515..1647
                     /label="intron chim_intron"
                     /note="chim_intron"
     primer_bind     1920..1943
                     /label="primer_bind BacMam seq2 primer"
                     /note="BacMam seq2 primer"
     primer_bind     1635..1658
                     /label="primer_bind BacMam seq1 primer"
                     /note="BacMam seq1 primer"
     CDS             1775..1798
                     /label="gene 3C protease site"
                     /note="3C protease site"
     CDS             1724..1762
                     /label="FLAG"
     misc_feature     1777..1828
                     /label="Found -> DNA"
                     /note="P565"
     misc_feature     complement(1750..1790)
                     /label="Found <- DNA"
                     /note="P570"
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa 
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg 
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc 
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt 
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa 
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag 
      361 gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac 
      421 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 
      481 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 
      541 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 
      601 tatggctgat tatgatcctc tagtacttct cgacaagctt ggtaccccta ggagatcttc 
      661 aatattggcc attagccata ttattcattg gttatatagc ataaatcaat attggctatt 
      721 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa 
      781 tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca attacggggt 
      841 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 
      901 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 
      961 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 
     1021 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg 
     1081 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc 
     1141 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca 
     1201 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 
     1261 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg 
     1321 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 
     1381 gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg 
     1441 ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt 
     1501 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac 
     1561 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg 
     1621 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag 
     1681 gctagagtac ttaatacgac tcactatagg gatcgccggc accatgggtg actacaagga 
     1741 cgacgacgac aagagcagcg gcatgggcag cagcttggaa gttctgtttc aagggccagc 
     1801 tagcgaattc gtcgactcta gactcgaggc atgcgcggcc gcatagaatc aacctctgga 
     1861 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 
     1921 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 
     1981 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 
     2041 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 
     2101 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 
     2161 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 
     2221 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 
     2281 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 
     2341 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 
     2401 gacgagtcgg atctcccttt gggccgcctc cccgcctgag acatgataag atacattgat 
     2461 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt 
     2521 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat 
     2581 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa 
     2641 aacctctaca aatgtggtag gatccgcgcc cgatggtggg acggtatgaa taatccggaa 
     2701 tatttatagg tttttttatt acaaaactgt tacgaaaaca gtaaaatact tatttatttg 
     2761 cgagatggtt atcattttaa ttatctccat gatctattaa tattccggag tatacgtagc 
     2821 caaccactag aactatagct agagtcctgg gcgaacaaac gatgctcgcc ttccagaaaa 
     2881 ccgaggatgc gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga 
     2941 ggtcttccga tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac 
     3001 agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc 
     3061 caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg 
     3121 aaacggatga aggcacgaac ccagttgaca taagcctgtt cggttcgtaa actgtaatgc 
     3181 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac 
     3241 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg 
     3301 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac 
     3361 gatgttacgc agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagg 
     3421 tggctcaagt atgggcatca ttcgcacatg taggctcggc cctgaccaag tcaaatccat 
     3481 gcgggctgct cttgatcttt tcggtcgtga gttcggagac gtagccacct actcccaaca 
     3541 tcagccggac tccgattacc tcgggaactt gctccgtagt aagacattca tcgcgcttgc 
     3601 tgccttcgac caagaagcgg ttgttggcgc tctcgcggct tacgttctgc ccaggtttga 
     3661 gcagccgcgt agtgagatct atatctatga tctcgcagtc tccggcgagc accggaggca 
     3721 gggcattgcc accgcgctca tcaatctcct caagcatgag gccaacgcgc ttggtgctta 
     3781 tgtgatctac gtgcaagcag attacggtga cgatcccgca gtggctctct atacaaagtt 
     3841 gggcatacgg gaagaagtga tgcactttga tatcgaccca agtaccgcca cctaacaatt 
     3901 cgttcaagcc gagatcggct tcccggccgc ggagttgttc ggtaaattgt cacaacgccg 
     3961 cgaatatagt ctttaccatg cccttggcca cgcccctctt taatacgacg ggcaatttgc 
     4021 acttcagaaa atgaagagtt tgctttagcc ataacaaaag tccagtatgc tttttcacag 
     4081 cataactgga ctgatttcag tttacaacta ttctgtctag tttaagactt tattgtcata 
     4141 gtttagatct attttgttca gtttaagact ttattgtccg cccacacccg cttacgcagg 
     4201 gcatccattt attactcaac cgtaaccgat tttgccaggt tacgcggctg gtctgcggtg 
     4261 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 
     4321 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     4381 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     4441 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     4501 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     4561 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     4621 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     4681 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     4741 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     4801 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     4861 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     4921 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     4981 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     5041 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     5101 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     5161 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     5221 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     5281 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     5341 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     5401 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     5461 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     5521 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     5581 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     5641 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     5701 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     5761 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     5821 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
     5881 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     5941 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     6001 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     6061 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     6121 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     6181 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 
     6241 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 
     6301 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 
     6361 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 
     6421 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 
     6481 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 
     6541 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 
     6601 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 
     6661 acccgccgcg cttaatgcgc cgctacaggg cgcgtc
//