LOCUS       pHN781_pET15b_H8        5938 bp    DNA     circular     05-Nov-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     Terminator      5623..5751
                     /label="Terminator T7_transcription_termination_region"
                     /note="T7"
     Other           5293..5309
                     /label="Other T7_gene_10_translational_enhancer_RBS"
                     /note="T7_transl_en_RBS"
     Regulatory      5248..5275
                     /label="Regulatory lac_operator"
                     /note="lacO"
     Promoter        5230..5248
                     /label="Promoter T7_promoter"
                     /note="Pr_T7"
     Regulatory      complement(3761..4852)
                     /label="Regulatory lacI_ORF"
                     /note="lacI"
     Other           complement(2258..2449)
                     /label="Other ROP_ORF"
                     /note="ROP"
     Origin          1224..1843
                     /label="Origin pBR322_replication_origin"
                     /note="Ori_pBR322"
     Selection       209..1069
                     /label="Selection ampicillin_resistance_gene_ORF"
                     /note="amp"
     Promoter        139..167
                     /label="Promoter ampicillin_resistance_gene(b-lactamase)promoter"
                     /note="Pr_amp"
     misc_feature     5199..5222
                     /label="P186 1"
                     /note="P186"
     misc_feature     5709..5733
                     /label="P187 1"
                     /note="P187"
     CDS             5323..5616
                     /label="His8_TEV_hSapA_60-140"
     misc_feature     complement(5280..5334)
                     /label="P314 1"
                     /note="P314"
     misc_feature     5317..5341
                     /label="P328 1"
                     /note="P328"
     misc_feature     complement(5602..5647)
                     /label="P31 1"
                     /note="P315"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact 
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc 
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga 
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc 
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg 
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac 
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga 
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg 
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc 
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg 
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt 
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt 
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg 
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat 
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc 
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt 
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat 
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg 
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg 
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg 
     3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc 
     3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca 
     3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat 
     4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc 
     4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct 
     4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt 
     4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac 
     4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga 
     4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg 
     4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag 
     4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga 
     4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca 
     4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg 
     4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt 
     4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc 
     4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga 
     4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt 
     4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt 
     4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg 
     4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac 
     5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg 
     5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca 
     5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc 
     5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag 
     5281 aaataatttt gtttaacttt aagaaggaga tataccggat ccatgcacca tcaccatcat 
     5341 catcaccatg aaaacctgta ctttcagggg agcttaccgt gtgacatttg caaagatgtg 
     5401 gttactgcag ctggcgatat gctcaaggat aatgcgaccg aagaagagat tctggtgtat 
     5461 ctggagaaaa cgtgtgattg gttgccaaaa ccgaacatgt cagcgtcgtg caaagagatc 
     5521 gttgacagct atcttccggt aattctggac atcatcaaag gcgaaatgtc tcgccctggt 
     5581 gaagtctgta gtgccctgaa tctgtgcgaa tcctgactcg agctgctaac aaagcccgaa 
     5641 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct 
     5701 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatcccgcaa 
     5761 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc 
     5821 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt 
     5881 taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
//