LOCUS       pCAGGS-chGrb2/eDHFR(69K6)-iRFP713 6846 bp ds-DNA     circular     30-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    7..1718
                     /label="CAG promotor"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1752..3689
                     /label="Translation 1752-3689"
     misc_feature    1755..1931
                     /label="Grb2(1-59)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1932..1976
                     /label="15aa linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1977..2471
                     /label="eDHFR(69K6)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2472..2507
                     /label="12aa linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2508..2702
                     /label="Grb2(152-216)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2529..2531
                     /label="Y160E"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2703..2741
                     /label="13aa linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2745..3689
                     /label="iRFP713"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3690..3692
                     /label="Translation 3690-3692"
     misc_feature    3690..3692
                     /label="termination"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3866..4330
                     /label="Rabbit b-globin 3'-flanking sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4345..4365)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(4371..4393)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(4397..4427)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(5078..5760)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(5858..6517)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcatagta GCTAGCagtt
     1741 gCTCGACCAC CATGGAAGCC ATCGCCAAAT ATGACTTCAA AGCTACTGCT GACGATGAGC
     1801 TGAGCTTCAA GAGGGGGGAC ATCCTTAAGG TTTTGAATGA AGAGTGTGAT CAGAACTGGT
     1861 ATAAGGCAGA ACTCAATGGG AAAGATGGCT TCATCCCCAA GAATTACATA GAAATGAAAC
     1921 CACATCCGTG GCGGcccggg TACAAGTCTG GGTCCGCAGC CAGTAGCGCC GGAAGTATGA
     1981 TCAGTCTGAT TGCGGCGTTA GCGGTAGATC GCGTTATCGG CATGGAAAAC GCCATGCCGT
     2041 GGAACCTGCC TGCCGATCTC GCCTGGTTTA AACGCAACAC CTTAAATAAA CCCGTGATTA
     2101 TGGGCCGCCA TACCTGGGAA TCAATCGGTC GTCCGTTGCC AGGACGCAAA AATATTATCC
     2161 TCAGCAGTCA ACCGGGTACG GACAAAAAAA AGAAAAAGAA AGATCGCGTA ACGTGGGTGA
     2221 AGTCGGTGGA TGAAGCCATC GCGGCGTGTG GTGACGTACC AGAAATCATG GTGATTGGCG
     2281 GCGGTCGCGT TTATGAACAG TTCTTGCCAA AAGCGCAAAA ACTGTATCTG ACGCATATCG
     2341 ACGCAGAAGT GGAAGGCGAC ACCCATTTCC CGGATTACGA GCCGGATGAC TGGGAATCGG
     2401 TATTCAGCGA ATTCCACGAT GCTGATGCGC AGAACTCTCA CAGCTATTGC TTTGAGATTC
     2461 TGGAGCGGCG Gctcgagtcg ggtggcggcg gaTCTtcAGC TggtGCTCAG GTGCCACAGC
     2521 AGCCAACGga aGTCCAGGCA CTCTTTGACT TTGACCCCCA GGAGGATGGT GAGCTGGGCT
     2581 TTCGCCGAGG AGACTTCATT CATGTCATGG ATAACTCAGA TCCCAATTGG TGGAAAGGGG
     2641 CCTGCCATGG GCAGACCGGC ATGTTTCCCC GCAATTATGT CACCCCAGTG AACCGGAACG
     2701 TCggcggccg cTCTGGTGCT GGTAGTGGCG CTGGTTCCGG TATGGCCGAA GGGAGCGTGG
     2761 CAAGACAGCC CGATCTGCTG ACATGCGACG ACGAACCAAT CCACATTCCA GGAGCTATCC
     2821 AGCCTCATGG ACTGCTGCTG GCTCTGGCCG CTGACATGAC TATCGTGGCA GGCTCTGATA
     2881 ACCTGCCAGA GCTGACCGGG CTGGCTATCG GAGCACTGAT TGGCCGGTCC GCAGCCGACG
     2941 TGTTCGATTC TGAGACTCAC AATAGACTGA CCATCGCCCT GGCTGAACCT GGGGCTGCAG
     3001 TGGGAGCCCC AATTACCGTC GGCTTCACAA TGCGAAAGGA CGCTGGCTTT ATCGGGTCCT
     3061 GGCACCGCCA TGATCAGCTG ATTTTTCTGG AGCTGGAACC ACCTCAGAGA GACGTGGCAG
     3121 AGCCACAGGC TTTCTTTCGG AGAACAAACA GCGCCATCAG GCGCCTGCAG GCCGCTGAGA
     3181 CTCTGGAATC CGCTTGCGCA GCCGCTGCAC AGGAAGTGAG GAAGATCACC GGGTTCGATC
     3241 GCGTCATGAT CTACAGATTC GCCTCAGACT TTAGCGGAGA AGTGATCGCA GAAGATCGCT
     3301 GTGCCGAGGT CGAAAGTAAA CTGGGCCTGC ATTACCCAGC CTCAACCGTG CCTGCACAGG
     3361 CACGACGGCT GTATACAATC AACCCCGTGC GGATCATTCC CGACATTAAT TACAGACCAG
     3421 TGCCCGTCAC ACCTGACCTG AACCCAGTGA CTGGGAGGCC CATCGATCTG AGCTTCGCCA
     3481 TTCTGCGCAG CGTGTCCCCT GTCCACCTGG AGTTTATGAG GAATATCGGA ATGCATGGCA
     3541 CCATGTCTAT CAGTATTCTG AGAGGCGAAA GGCTGTGGGG GCTGATCGTG TGCCACCATC
     3601 GGACACCTTA CTATGTGGAC CTGGACGGCA GGCAGGCTTG TGAACTGGTG GCTCAGGTGC
     3661 TGGCTTGGCA GATCGGCGTG ATGGAAGAGT AGgagctcca gcttttgttc cctttAGTGa
     3721 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttccTGTGTG AAATTGTTAT
     3781 CCGCTaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     3841 tgccctggct cacaaatacc actgaGATCT TTTTCCCTCT GCCAAAAATT ATGGGGACAT
     3901 CATGAAGCCC CTTGAGCATC TGACTTCTGG CTAATAAAGG AAATTTATTT TCATTGCAAT
     3961 AGTGTGTTGG AATTTTTTGT GTCTCTCACT CGGAAGGACA TATGGGAGGG CAAATCATTT
     4021 AAAACATCAG AATGAGTATT TGGTTTAGAG TTTGGCAACA TATGCCCATA TGCTGGCTGC
     4081 CATGAACAAA GGTTGGCTAT AAAGAGGTCA TCAGTATATG AAACAGCCCC CTGCTGTCCA
     4141 TTCCTTATTC CATAGAAAAG CCTTGACTTG AGGTTAGATT TTTTTTATAT TTTGTTTTGT
     4201 GTTATTTTTT TCTTTAACAT CCCTAAAATT TTCCTTACAT GTTTTACTAG CCAGATTTTT
     4261 CCTCCTCTCC TGACTACTCC CAGTCATAGC TGTCCCTCTT CTCTTATGGA GATCCCTCGA
     4321 CCTGCAGCCC aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4381 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4441 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4501 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4561 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4621 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4681 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     4741 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4801 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     4861 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4921 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4981 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5041 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5101 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5161 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5221 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5281 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5341 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5401 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5461 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5521 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5581 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5641 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5701 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5761 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5821 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5881 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5941 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6001 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6061 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6121 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6181 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6241 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6301 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6361 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6421 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6481 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6541 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6601 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6661 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6721 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6781 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6841 cacctg
//