LOCUS       pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670 8372 bp ds-DNA     circular     30-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    7..1718
                     /label="CAG promotor"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1754..5218
                     /label="Translation 1754-5218"
     misc_feature    1757..2443
                     /label="PKCdelta(1-229)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2444..2479
                     /label="12aa linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2480..2974
                     /label="eDHFR(69K6)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2975..3010
                     /label="12aa linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3011..4198
                     /label="PKCdelta(280-675)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    4274..5215
                     /label="miRFP670"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5216..5218
                     /label="termination"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5392..5856
                     /label="Rabbit b-globin 3'-flanking sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(5871..5891)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(5897..5919)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(5923..5953)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(6604..7286)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(7384..8043)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcatagta GCTAGCagtt
     1741 gCTCGAGGCC ACCATGGCGC CGTTCCTGCG CATCGCCTTC AACTCCTATG AGCTGGGCTC
     1801 CCTGCAGGCC GAGGACGAGG CGAACCAGCC CTTCTGTGCC GTGAAGATGA AGGAGGCGCT
     1861 CAGCACAGAG CGTGGGAAAA CACTGGTGCA GAAGAAGCCG ACCATGTATC CTGAGTGGAA
     1921 GTCGACGTTC GACGCCCACA TCTATGAGGG GCGCGTCATC CAGATTGTGC TAATGCGGGC
     1981 AGCAGAGGAG CCAGTGTCTG AGGTGACCGT GGGTGTGTCG GTGCTGGCCG AGCGCTGCAA
     2041 GAAGAACAAT GGCAAGGCTG AGTTCTGGCT GGACCTGCAG CCTCAGGCCA AGGTGTTGAT
     2101 GTCTGTTCAG TATTTCCTGG AGGACGTGGA TTGCAAACAG TCTATGCGCA GTGAGGACGA
     2161 GGCCAAGTTC CCAACGATGA ACCGCCGCGG AGCCATCAAA CAGGCCAAAA TCCACTACAT
     2221 CAAGAACCAT GAGTTTATCG CCACCTTCTT TGGGCAACCC ACCTTCTGTT CTGTGTGCAA
     2281 AGACTTTGTC TGGGGCCTCA ACAAGCAAGG CTACAAATGC AGGCAATGTA ACGCTGCCAT
     2341 CCACAAGAAA TGCATCGACA AGATCATCGG CAGATGCACT GGCACCGCGG CCAACAGCCG
     2401 GGACACTATA TTCCAGAAAG AACGCTTCAA CATCGACATG CCGaccggtT CTGGGTCCGC
     2461 AGCCAGTAGC GCCGGAAGTA TGATCAGTCT GATTGCGGCG TTAGCGGTAG ATCGCGTTAT
     2521 CGGCATGGAA AACGCCATGC CGTGGAACCT GCCTGCCGAT CTCGCCTGGT TTAAACGCAA
     2581 CACCTTAAAT AAACCCGTGA TTATGGGCCG CCATACCTGG GAATCAATCG GTCGTCCGTT
     2641 GCCAGGACGC AAAAATATTA TCCTCAGCAG TCAACCGGGT ACGGACAAAA AAAAGAAAAA
     2701 GAAAGATCGC GTAACGTGGG TGAAGTCGGT GGATGAAGCC ATCGCGGCGT GTGGTGACGT
     2761 ACCAGAAATC ATGGTGATTG GCGGCGGTCG CGTTTATGAA CAGTTCTTGC CAAAAGCGCA
     2821 AAAACTGTAT CTGACGCATA TCGACGCAGA AGTGGAAGGC GACACCCATT TCCCGGATTA
     2881 CGAGCCGGAT GACTGGGAAT CGGTATTCAG CGAATTCCAC GATGCTGATG CGCAGAACTC
     2941 TCACAGCTAT TGCTTTGAGA TTCTGGAGCG GCGGggtGCT tcgggtggcg gcggaTCTtc
     3001 AGCTcccggg GGCATCAACC AGAAGCTTTT GGCTGAGGCC TTGAACCAAG TCACCCAGAG
     3061 AGCCTCCCGG AGATCAGACT CAGCCTCCTC AGAGCCTGTT GGGATATATC AGGGTTTCGA
     3121 GAAGAAGACC GGAGTTGCTG GGGAGGACAT GCAAGACAAC AGTGGGACCT ACGGCAAGAT
     3181 CTGGGAGGGC AGCAGCAAGT GCAACATCAA CAACTTCATC TTCCACAAGG TCCTGGGCAA
     3241 AGGCAGCTTC GGGAAGGTGC TGCTTGGAGA GCTGAAGGGC AGAGGAGAGT ACTTTGCCAT
     3301 CAAGGCCCTC AAGAAGGATG TGGTCCTGAT CGACGACGAC GTGGAGTGCA CCATGGTTGA
     3361 GAAGCGGGTG CTGACACTTG CCGCAGAGAA TCCCTTTCTC ACCCACCTCA TCTGCACCTT
     3421 CCAGACCAAG GACCACCTGT TCTTTGTGAT GGAGTTCCTC AACGGGGGGG ACCTGATGTA
     3481 CCACATCCAG GACAAAGGCC GCTTTGAACT CTACCGTGCC ACGTTTTATG CCGCTGAGAT
     3541 AATGTGTGGA CTGCAGTTTC TACACAGCAA GGGCATCATT TACAGGGACC TCAAACTGGA
     3601 CAATGTGCTG TTGGACCGGG ATGGCCACAT CAAGATTGCC GACTTTGGGA TGTGCAAAGA
     3661 GAACATATTC GGGGAGAGCC GGGCCAGCAC CTTCTGCGGC ACCCCTGACT ATATCGCCCC
     3721 TGAGATCCTA CAGGGCCTGA AGTACACATT CTCTGTGGAC TGGTGGTCTT TCGGGGTCCT
     3781 TCTGTACGAG ATGCTCATTG GCCAGTCCCC CTTCCATGGT GATGATGAGG ATGAACTCTT
     3841 CGAGTCCATC CGTGTGGACA CGCCACATTA TCCCCGCTGG ATCACCAAGG AGTCCAAGGA
     3901 CATCCTGGAG AAGCTCTTTG AAAGGGAACC AACCAAGAGG CTGGGAGTGA CGGGAAACAT
     3961 CAAAATCCAC CCCTTCTTCA AGACCATAAA CTGGACTCTG CTGGAAAAGC GGAGGTTGGA
     4021 GCCACCCTTC AGGCCCAAAG TGAAGTCACC CAGAGACTAC AGTAACTTTG ACCAGGAGTT
     4081 CCTGAACGAG AAGGCGCGCC TCTCCTACAG CGACAAGAAC CTCATCGACT CCATGGACCA
     4141 GTCTGCATTC GCTGGCTTCT CCTTTGTGAA CCCCAAATTC GAGCACCTCC TGGAAGATGC
     4201 GGCCGCtGGT AGTGGTGCTG GCTCTGGTGC TGGTAGTGGC GCTGGTTCCG GTGCTGGCTC
     4261 TtCGGCCGCt atggtagcag gtcatgcctc tggcagcccc gcattcggga ccgcctctca
     4321 ttcgaattgc gaacatgaag agatccacct cgccggctcg atccagccgc atggcgcgct
     4381 tctggtcgtc agcgaacatg atcatcgcgt catccaggcc agcgccaacg ccgcggaatt
     4441 tctgaatctc ggaagcgtac tcggcgttcc gctcgccgag atcgacggcg atctgttgat
     4501 caagatcctg ccgcatctcg atcccaccgc cgaaggcatg ccggtcgcgg tgcgctgccg
     4561 gatcggcaat ccctctacgg agtactgcgg tctgatgcat cggcctccgg aaggcgggct
     4621 gatcatcgaa ctcgaacgtg ccggcccgtc gatcgatctg tcaggcacgc tggcgccggc
     4681 gctggagcgg atccgcacgg cgggttcact gcgcgcgctg tgcgatgaca ccgtgctgct
     4741 gtttcagcag tgcaccggct acgaccgggt gatggtgtat cgtttcgatg agcaaggcca
     4801 cggcctggta ttctccgagt gccatgtgcc tgggctcgaa tcctatttcg gcaaccgcta
     4861 tccgtcgtcg actgtcccgc agatggcgcg gcagctgtac gtgcggcagc gcgtccgcgt
     4921 gctggtcgac gtcacctatc agccggtgcc gctggagccg cggctgtcgc cgctgaccgg
     4981 gcgcgatctc gacatgtcgg gctgcttcct gcgctcgatg tcgccgtgcc atctgcagtt
     5041 cctgaaggac atgggcgtgc gcgccaccct ggcggtgtcg ctggtggtcg gcggcaagct
     5101 gtggggcctg gttgtctgtc accattatct gccgcgcttc atccgtttcg agctgcgggc
     5161 gatctgcaaa cggctcgccg aaaggatcgc gacgcggatc accgcgcttg agagcTAgGa
     5221 gctccagctt ttgttccctt tAGTGagggt taattgcgcg cttggcgtaa tcatggtcat
     5281 agctgtttcc TGTGTGAAAT TGTTATCCGC Taattcactc ctcaggtgca ggctgcctat
     5341 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg aGATCTTTTT
     5401 CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA
     5461 TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA
     5521 AGGACATATG GGAGGGCAAA TCATTTAAAA CATCAGAATG AGTATTTGGT TTAGAGTTTG
     5581 GCAACATATG CCCATATGCT GGCTGCCATG AACAAAGGTT GGCTATAAAG AGGTCATCAG
     5641 TATATGAAAC AGCCCCCTGC TGTCCATTCC TTATTCCATA GAAAAGCCTT GACTTGAGGT
     5701 TAGATTTTTT TTATATTTTG TTTTGTGTTA TTTTTTTCTT TAACATCCCT AAAATTTTCC
     5761 TTACATGTTT TACTAGCCAG ATTTTTCCTC CTCTCCTGAC TACTCCCAGT CATAGCTGTC
     5821 CCTCTTCTCT TATGGAGATC CCTCGACCTG CAGCCCaagc ttggcgtaat catggtcata
     5881 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5941 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6001 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag
     6061 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6121 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6181 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6241 aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6301 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6361 atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg
     6421 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6721 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6781 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     6841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7021 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     7081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7261 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7321 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7981 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8041 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8341 tccgcgcaca tttccccgaa aagtgccacc tg
//