LOCUS       pCMV-FKBP-mTagBFP2-SOSc 6498 bp ds-DNA     circular     02-NOV-2021
DEFINITION  .
KEYWORDS    "Creator:Masaru Yoshikawa" "Deposit:BOX15-5A"                      
FEATURES             Location/Qualifiers
     promoter        1..304
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        305..508
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             544..3084
                     /label="Translation 544-3084"
     CDS             547..867
                     /label="FKBP12"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             898..1608
                     /label="mTagBFP2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1630..2184
                     /label="Rem domain"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1630..3081
                     /label="hSOS1 cat (566-1049)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2185..3081
                     /label="Cdc25 domain"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(3486..3792)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        3983..4299
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        4103..4299
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             4337..5128
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      5660..6342
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
       61 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
      121 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
      181 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
      241 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
      301 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
      361 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
      421 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
      481 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCCGCC
      541 ACCATGGGAG TGCAGGTGGA AACCATCTCC CCAGGAGACG GGCGCACCTT CCCCAAGCGC
      601 GGCCAGACCT GCGTGGTGCA CTACACCGGG ATGCTTGAAG ATGGAAAGAA ATTTGATTCC
      661 TCCCGGGACA GAAACAAGCC CTTTAAGTTT ATGCTAGGCA AGCAGGAGGT GATCCGAGGC
      721 TGGGAAGAAG GGGTTGCCCA GATGAGTGTG GGTCAGAGAG CCAAACTGAC TATATCTCCA
      781 GATTATGCCT ATGGTGCCAC TGGGCACCCA GGCATCATCC CACCACATGC CACTCTCGTC
      841 TTCGATGTGG AGCTTCTAAA ACTGGAAGGC TCCGGTGCCA GTGCAccggt cgccaccatg
      901 gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc
      961 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     1021 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     1081 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
     1141 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     1201 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     1261 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
     1321 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
     1381 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca
     1441 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
     1501 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
     1561 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatAG ATCTCGAGCT
     1621 CAAGCTTCGc agatgaggct gcctagtgct gatgtttata gatttgcaga gcctgactct
     1681 gaagagaata ttatatttga agagaacatg cagcccaagg ctggaattcc aattatcaaa
     1741 gcaggaactg ttattaaact tatagagagg cttacgtacc atatgtacgc agatcccaat
     1801 tttgttcgga catttcttac aacatacaga tccttttgca aacctcaaga actactgagt
     1861 cttataatag aaaggtttga aattccagag cctgagccaa cagaagctga tcgcatagct
     1921 atagagaatg gagatcaacc cttgagtgca gaactgaaaa gatttagaaa agaatatata
     1981 cagcctgtgc aactgcgagt attaaatgta tgtcggcact gggtagagca ccacttctat
     2041 gattttgaaa gagatgcata tcttttgcaa cgaatggaag aatttattgg aacagtaaga
     2101 ggtaaagcaa tgaaaaaatg ggttgaatcc atcactaaaa taatccaaag gaaaaaaatt
     2161 gcaagagaca atggaccagg tcataatatt acatttcaga gttcacctcc cacagttgag
     2221 tggcatataa gcagacctgg gcacatagag acttttgacc tgctcacctt acacccaata
     2281 gaaattgctc gacaactcac tttacttgaa tcagatctat accgagctgt acagccatca
     2341 gaattagttg gaagtgtgtg gacaaaagaa gacaaagaaa ttaactctcc taatcttctg
     2401 aaaatgattc gacataccac caacctcact ctgtggtttg agaaatgtat tgtagaaact
     2461 gaaaatttag aagaaagagt agctgtggtg agtcgaatta ttgagattct acaagtcttt
     2521 caagagttga acaactttaa tggtgtcctt gaggttgtca gtgctatgaa ttcatcacct
     2581 gtttacagac tagaccacac atttgagcaa ataccaagtc gccagaagaa aattttagaa
     2641 gaagctcatg aattgagtga agatcactat aagaaatatt tggcaaaact caggtctatt
     2701 aatccaccat gtgtgccttt ctttggaatt tatctcacta atatcttgaa aacagaagaa
     2761 ggcaaccctg aggtcctaaa aagacatgga aaagagctta taaactttag caaaaggagg
     2821 aaagtagcag aaataacagg agagatccag cagtaccaaa atcagcctta ctgtttacga
     2881 gtagaatcag atatcaaaag gttctttgaa aacttgaatc cgatgggaaa tagcatggag
     2941 aaggaattta cagattatct tttcaacaaa tccctagaaa tagaaccacg aaaccctaag
     3001 cctctcccaa gatttccaaa aaaatatagc tatcccctaa aatctcctgg tgttcgtcca
     3061 tcaaacccaa gaccaggtac ctaaaCCGCG GGCCCGGGAT CCACCGGATC TAGATAACTG
     3121 ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC
     3181 CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA
     3241 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
     3301 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAACG CGTAAATTGT
     3361 AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA
     3421 CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT
     3481 GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA
     3541 AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG
     3601 TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT
     3661 TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG
     3721 AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC
     3781 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG
     3841 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA
     3901 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG AAAGAACCAG
     3961 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
     4021 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA
     4081 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
     4141 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
     4201 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
     4261 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG
     4321 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
     4381 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
     4441 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
     4501 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
     4561 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
     4621 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
     4681 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
     4741 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
     4801 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
     4861 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
     4921 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA
     4981 CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG
     5041 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT
     5101 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA
     5161 GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
     5221 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
     5281 CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG
     5341 GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG
     5401 TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA
     5461 CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG
     5521 GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCCTCAGGT
     5581 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG
     5641 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA
     5701 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA
     5761 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA
     5821 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT
     5881 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA
     5941 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT
     6001 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG
     6061 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG
     6121 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA
     6181 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT
     6241 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG
     6301 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
     6361 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
     6421 CGTATTACCG CCATGCATTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG
     6481 CCCATATATG GAGTTCCG
//