LOCUS       pCMV-PB1-AG-FRB-TEVcs-m 6498 bp ds-DNA     circular     26-OCT-2021
DEFINITION  .
KEYWORDS    "Creator:Masahiro Ikuta" "Deposit:BOX16-1C"                        
FEATURES             Location/Qualifiers
     promoter        1..304
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        305..508
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             546..3101
                     /label="Translation 546-3101"
     CDS             549..851
                     /label="PB1-p62(Ash)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             1233..1907
                     /label="Azami-Green"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1959..2237
                     /label="FRB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2283..2303
                     /label="TEVCs-Low"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2343..3053
                     /label="mTagBFP2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3066..3092
                     /label="HA tag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3486..3792)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        3983..4299
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        4103..4299
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             4337..5128
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      5660..6342
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
       61 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
      121 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
      181 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
      241 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
      301 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
      361 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
      421 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
      481 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCATTG
      541 CCACCATGGC GTCGCTCACC GTGAAGGCCT ACCTTCTGGG CAAGGAGGAC GCGGCGCGCG
      601 AGATTCGCCG CTTCAGCTTC TGTTGCAGCC CCGAGCCTGA GGCGGAAGCC GAGGCTGCGG
      661 CGGGTCCGGG ACCCTGCGAG CGGCTGCTGA GCCGGGTGGC CGCCCTGTTC CCCGCGCTGC
      721 GGCCTGGCGG CTTCCAGGCG CACTACCGCG ATGAGGACGG GGACTTGGTT GCCTTTTCCA
      781 GTGACGAGGA ATTGACAATG GCCATGTCCT ACGTGAAGGA TGACATCTTC CGAATCTACA
      841 TTAAAGAGAA AACCGGTTCT GGGAGTGGCG GTAGTGGTGC TGGAGGCAGC GCAGGCTCTG
      901 GAGCAGGCGG CAGTGCCGGA AGTGGCGCTG GAGGGTCTGC CGGATCTGGA GCCGGTGGTT
      961 CTGCCGGCTC CGGCGCTGGT GGGAGCGCTT CCGGAAGTAG TCCCGGTTCT GGGAGTGGCG
     1021 GTAGTGGTGC TGGAGGCAGC GCAGGCTCTG GAGCAGGCGG CAGTGCCGGA AGTGGCGCTG
     1081 GAGGGTCTGC CGGATCTGGA GCCGGTGGTT CTGCCGGCTC CGGCGCTGGT GGGAGCGCTT
     1141 CCGGAAGTAG TCCCGGTAAT TCCGCTGACG GCGGCGGAGG ATCGGGTGGT AGTGGTGGTT
     1201 CAGGAGGAGG ATCGACCCAA GGAGGATCCA TGGTGAGCGT GATCAAGCCC GAGATGAAGA
     1261 TCAAGCTGTG CATGAGGGGC ACCGTGAACG GCCACAACTT CGTGATCGAG GGCGAGGGCA
     1321 AGGGCAACCC CTACGAGGGC ACCCAGATCC TGGACCTGAA CGTGACCGAG GGCGCCCCCC
     1381 TGCCCTTCGC CTACGACATC CTGACCACCG TGTTCCAGTA CGGCAACAGG GCCTTCACCA
     1441 AGTACCCCGC CGACATCCAG GACTACTTCA AGCAGACCTT CCCCGAGGGC TACCACTGGG
     1501 AGAGGAGCAT GACCTACGAG GACCAGGGCA TCTGCACCGC CACCAGCAAC ATCAGCATGA
     1561 GGGGCGACTG CTTCTTCTAC GACATCAGGT TCGACGGCGT GAACTTCCCC CCCAACGGCC
     1621 CCGTGATGCA GAAGAAGACC CTGAAGTGGG AGCCCAGCAC CGAGAAGATG TACGTGAGGG
     1681 ACGGCGTGCT GAAGGGCGAC GTGAACATGG CCCTGCTGCT GGAGGGCGGC GGCCACTACA
     1741 GGTGCGACTT CAAGACCACC TACAAGGCCA AGAAGGACGT GAGGCTGCCC GACTACCACT
     1801 TCGTGGACCA CAGGATCGAG ATCCTGAAGC ACGACAAGGA CTACAACAAG GTGAAGCTGT
     1861 ACGAGAACGC CGTGGCCAGG TACAGCATGC TGCCCAGCCA GGCCAAGGGT ACCGGAACTG
     1921 CAGCAGAGAA TTCGGGAAAC TCGAGAACAA AGCTTATGAT CCTCTGGCAT GAGATGTGGC
     1981 ATGAAGGCCT GGAAGAGGCA TCTCGTTTGT ACTTTGGGGA AAGGAACGTG AAAGGCATGT
     2041 TTGAGGTGCT GGAGCCCTTG CATGCTATGA TGGAACGGGG CCCCCAGACT CTGAAGGAAA
     2101 CATCCTTTAA TCAGGCCTAT GGTCGAGATT TAATGGAGGC CCAAGAGTGG TGCAGGAAGT
     2161 ACATGAAATC AGGGAATGTC AAGGACCTCC TCCAAGCCTG GGACCTCTAT TATCATGTGT
     2221 TCCGACGAAT CTCAAAGCCC GGGGGTAGTG GTGCTGGCTC TGGTGCTGGT AGTGGCGCTG
     2281 GTGAAAACCT GTATTTTCAG CTGGTCGATG GTGGTAGTGC TGGTGGTTCC GGAGGTGTCG
     2341 ACatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg
     2401 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     2461 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     2521 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     2581 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     2641 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     2701 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     2761 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     2821 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga
     2881 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     2941 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     3001 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aatAGATCCG
     3061 GCTCTTACCC ATACGATGTT CCAGATTACG CTAGATCTTA AAAAGCGGCC GCGACTCTAG
     3121 ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC
     3181 CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA
     3241 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
     3301 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAAGG CGTAAATTGT
     3361 AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA
     3421 CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT
     3481 GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA
     3541 AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG
     3601 TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT
     3661 TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG
     3721 AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC
     3781 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG
     3841 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA
     3901 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG AAAGAACCAG
     3961 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
     4021 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA
     4081 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
     4141 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
     4201 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
     4261 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG
     4321 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
     4381 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
     4441 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
     4501 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
     4561 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
     4621 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
     4681 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
     4741 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
     4801 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
     4861 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
     4921 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA
     4981 CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG
     5041 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT
     5101 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA
     5161 GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
     5221 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
     5281 CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG
     5341 GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG
     5401 TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA
     5461 CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG
     5521 GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCCTCAGGT
     5581 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG
     5641 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA
     5701 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA
     5761 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA
     5821 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT
     5881 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA
     5941 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT
     6001 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG
     6061 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG
     6121 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA
     6181 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT
     6241 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG
     6301 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
     6361 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
     6421 CGTATTACCG CCATGCATTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG
     6481 CCCATATATG GAGTTCCG
//