LOCUS       pCMV-PB1-AG-FRB-TEVcs-m 7656 bp ds-DNA     circular     02-NOV-2021
DEFINITION  .
KEYWORDS    "Creator:Masahiro Ikuta" "Deposit:BOX16-1D"                        
FEATURES             Location/Qualifiers
     promoter        1..304
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        305..508
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             546..4250
                     /label="Translation 546-4250"
     CDS             549..851
                     /label="PB1-p62(Ash)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             1233..1907
                     /label="Azami-Green"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1959..2237
                     /label="FRB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2283..2303
                     /label="TEVCs-Low"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2343..3053
                     /label="mTagBFP2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3066..3092
                     /label="HA tag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             3105..4247
                     /label="mVav2 (183-563)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             3135..3671
                     /label="mVav2(DH)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3756..4064
                     /label="mVav2(PH)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             4083..4247
                     /label="mVav2(CRD)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(4644..4950)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        5141..5457
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        5261..5457
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             5495..6286
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      6818..7500
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
       61 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
      121 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
      181 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
      241 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
      301 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
      361 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
      421 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
      481 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCATTG
      541 CCACCATGGC GTCGCTCACC GTGAAGGCCT ACCTTCTGGG CAAGGAGGAC GCGGCGCGCG
      601 AGATTCGCCG CTTCAGCTTC TGTTGCAGCC CCGAGCCTGA GGCGGAAGCC GAGGCTGCGG
      661 CGGGTCCGGG ACCCTGCGAG CGGCTGCTGA GCCGGGTGGC CGCCCTGTTC CCCGCGCTGC
      721 GGCCTGGCGG CTTCCAGGCG CACTACCGCG ATGAGGACGG GGACTTGGTT GCCTTTTCCA
      781 GTGACGAGGA ATTGACAATG GCCATGTCCT ACGTGAAGGA TGACATCTTC CGAATCTACA
      841 TTAAAGAGAA AACCGGTTCT GGGAGTGGCG GTAGTGGTGC TGGAGGCAGC GCAGGCTCTG
      901 GAGCAGGCGG CAGTGCCGGA AGTGGCGCTG GAGGGTCTGC CGGATCTGGA GCCGGTGGTT
      961 CTGCCGGCTC CGGCGCTGGT GGGAGCGCTT CCGGAAGTAG TCCCGGTTCT GGGAGTGGCG
     1021 GTAGTGGTGC TGGAGGCAGC GCAGGCTCTG GAGCAGGCGG CAGTGCCGGA AGTGGCGCTG
     1081 GAGGGTCTGC CGGATCTGGA GCCGGTGGTT CTGCCGGCTC CGGCGCTGGT GGGAGCGCTT
     1141 CCGGAAGTAG TCCCGGTAAT TCCGCTGACG GCGGCGGAGG ATCGGGTGGT AGTGGTGGTT
     1201 CAGGAGGAGG ATCGACCCAA GGAGGATCCA TGGTGAGCGT GATCAAGCCC GAGATGAAGA
     1261 TCAAGCTGTG CATGAGGGGC ACCGTGAACG GCCACAACTT CGTGATCGAG GGCGAGGGCA
     1321 AGGGCAACCC CTACGAGGGC ACCCAGATCC TGGACCTGAA CGTGACCGAG GGCGCCCCCC
     1381 TGCCCTTCGC CTACGACATC CTGACCACCG TGTTCCAGTA CGGCAACAGG GCCTTCACCA
     1441 AGTACCCCGC CGACATCCAG GACTACTTCA AGCAGACCTT CCCCGAGGGC TACCACTGGG
     1501 AGAGGAGCAT GACCTACGAG GACCAGGGCA TCTGCACCGC CACCAGCAAC ATCAGCATGA
     1561 GGGGCGACTG CTTCTTCTAC GACATCAGGT TCGACGGCGT GAACTTCCCC CCCAACGGCC
     1621 CCGTGATGCA GAAGAAGACC CTGAAGTGGG AGCCCAGCAC CGAGAAGATG TACGTGAGGG
     1681 ACGGCGTGCT GAAGGGCGAC GTGAACATGG CCCTGCTGCT GGAGGGCGGC GGCCACTACA
     1741 GGTGCGACTT CAAGACCACC TACAAGGCCA AGAAGGACGT GAGGCTGCCC GACTACCACT
     1801 TCGTGGACCA CAGGATCGAG ATCCTGAAGC ACGACAAGGA CTACAACAAG GTGAAGCTGT
     1861 ACGAGAACGC CGTGGCCAGG TACAGCATGC TGCCCAGCCA GGCCAAGGGT ACCGGAACTG
     1921 CAGCAGAGAA TTCGGGAAAC TCGAGAACAA AGCTTATGAT CCTCTGGCAT GAGATGTGGC
     1981 ATGAAGGCCT GGAAGAGGCA TCTCGTTTGT ACTTTGGGGA AAGGAACGTG AAAGGCATGT
     2041 TTGAGGTGCT GGAGCCCTTG CATGCTATGA TGGAACGGGG CCCCCAGACT CTGAAGGAAA
     2101 CATCCTTTAA TCAGGCCTAT GGTCGAGATT TAATGGAGGC CCAAGAGTGG TGCAGGAAGT
     2161 ACATGAAATC AGGGAATGTC AAGGACCTCC TCCAAGCCTG GGACCTCTAT TATCATGTGT
     2221 TCCGACGAAT CTCAAAGCCC GGGGGTAGTG GTGCTGGCTC TGGTGCTGGT AGTGGCGCTG
     2281 GTGAAAACCT GTATTTTCAG CTGGTCGATG GTGGTAGTGC TGGTGGTTCC GGAGGTGTCG
     2341 ACatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg
     2401 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     2461 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     2521 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     2581 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     2641 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     2701 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     2761 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     2821 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga
     2881 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     2941 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     3001 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aatAGATCCG
     3061 GCTCTTACCC ATACGATGTT CCAGATTACG CTAGATCTCG AGGGCCCATG AAAATGGGCA
     3121 TGACTGAGGA CGACAAGAGA AGCTGCTGCT TGTTAGAGAT TCAGGAGACC GAGGCCAAGT
     3181 ACTACCGCAC CCTGGAGGAC ATTGAGAAGA ACTACATGGG TCCCTTGCGG CTGGTGCTGA
     3241 GCCCGGCGGA TATGGCTGCT GTCTTCATCA ACCTGGAGGA CCTCATCAAG GTGCATCACA
     3301 GCTTTCTGCG AGCCATCGAT GTGTCCATGA TGGCTGGTGG CAGTACCCTG GCTAAGGTCT
     3361 TTCTGGAGTT TAAGGAAAGG CTCCTGATCT ATGGAGAGTA CTGTAGCCAC ATGGAACACG
     3421 CTCAGAGTAC ACTGAACCAG CTCCTCGCCA GCCGAGAGGA CTTCAGGCAG AAAGTGGAGG
     3481 AGTGCACACT CAGGGTTCAG GATGGCAAGT TCAAGCTGCA AGACCTGCTG GTGGTGCCCA
     3541 TGCAACGGGT GCTGAAGTAC CACCTGCTGC TCAAGGAGCT CCTGAGCCAT TCTGCAGACC
     3601 GACCAGAAAG ACAACAGCTC AAAGAAGCCC TGGAAGCCAT GCAGGACTTG GCCATGTACA
     3661 TCAATGAAGT GAAGCGGGAC AAGGAGACCT TGAAGAAGAT TAGCGAGTTC CAGTGCTCCA
     3721 TAGAAAACCT GCAAGTGAAG CTGGAGGAAT TTGGGAGGCC AAAGATTGAC GGGGAGCTTA
     3781 AAGTCCGGTC CATAGTCAAC CACACCAAGC AAGACAGGTA CCTGTTCCTA TTTGACAAGG
     3841 TGGTCATCGT GTGCAAGAGG AAGGGCTACA GCTATGAGCT GAAGGAGGTC ATTGAGCTGC
     3901 TCTTCCACAA GATGACCGAT GACCCGATGC ACAACAAGGA CATCAAGAAG TGGTCCTATG
     3961 GCTTCTACCT GATTCACCTC CAAGGAAAGC AAGGCTTTCA GTTCTTCTGC AAGACGGAAG
     4021 ACATGAAGCG GAAGTGGATG GAGCAGTTCG AGATGGCCAT GTCAAACATC AAGCCAGATA
     4081 AGGCCAATGC CAACCATCAT AGCTTCCAGA TGTACACATT CGACAAGACT ACCAACTGCA
     4141 AAGCCTGCAA GATGTTTCTC AGGGGTACCT TCTACCAGGG ATACCTGTGT ACCAGATGTG
     4201 GCGTCGGGGC ACACAAGGAA TGCCTGGAGG TGATCCCCCC CTGCAAGTAA CCCGGGatac
     4261 tcGCGGCCGC GACTCTAGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT
     4321 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT
     4381 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
     4441 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
     4501 TCTTAAGGCG TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA
     4561 AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA
     4621 ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA
     4681 CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA
     4741 ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC
     4801 TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA
     4861 AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG
     4921 CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCAG GTGGCACTTT
     4981 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA
     5041 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTCC
     5101 TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC
     5161 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA
     5221 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA
     5281 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT
     5341 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC
     5401 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT
     5461 CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
     5521 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
     5581 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
     5641 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT
     5701 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
     5761 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
     5821 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
     5881 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
     5941 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
     6001 GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
     6061 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
     6121 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
     6181 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA
     6241 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG
     6301 TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC
     6361 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC
     6421 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCTA GGGGGAGGCT AACTGAAACA
     6481 CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA
     6541 ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT
     6601 GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA
     6661 CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC
     6721 CCTGCCATAG CCTCAGGTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
     6781 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
     6841 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
     6901 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
     6961 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
     7021 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
     7081 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
     7141 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     7201 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
     7261 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
     7321 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
     7381 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
     7441 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
     7501 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC
     7561 CCTGATTCTG TGGATAACCG TATTACCGCC ATGCATTAGT TATTAATAGT AATCAATTAC
     7621 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCG
//