LOCUS       pCMV-PB1-AG-FRB-TEVcs-m 7958 bp ds-DNA     circular     02-NOV-2021
DEFINITION  .
KEYWORDS    "Creator:Masahiro Ikuta" "Deposit:BOX23-4B"                        
FEATURES             Location/Qualifiers
     promoter        1..304
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        305..508
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             546..4553
                     /label="Translation 546-4553"
     CDS             549..851
                     /label="PB1-p62(Ash)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             1233..1907
                     /label="Azami-Green"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1959..2237
                     /label="FRB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2283..2303
                     /label="TEVCs-Low"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2343..3053
                     /label="mTagBFP2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3066..3092
                     /label="HA tag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3099..4550
                     /label="hSOS1 (566-1049)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(4946..5252)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        5443..5759
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        5563..5759
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             5797..6588
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      7120..7802
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
       61 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
      121 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
      181 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
      241 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
      301 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
      361 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
      421 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
      481 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCATTG
      541 CCACCATGGC GTCGCTCACC GTGAAGGCCT ACCTTCTGGG CAAGGAGGAC GCGGCGCGCG
      601 AGATTCGCCG CTTCAGCTTC TGTTGCAGCC CCGAGCCTGA GGCGGAAGCC GAGGCTGCGG
      661 CGGGTCCGGG ACCCTGCGAG CGGCTGCTGA GCCGGGTGGC CGCCCTGTTC CCCGCGCTGC
      721 GGCCTGGCGG CTTCCAGGCG CACTACCGCG ATGAGGACGG GGACTTGGTT GCCTTTTCCA
      781 GTGACGAGGA ATTGACAATG GCCATGTCCT ACGTGAAGGA TGACATCTTC CGAATCTACA
      841 TTAAAGAGAA AACCGGTTCT GGGAGTGGCG GTAGTGGTGC TGGAGGCAGC GCAGGCTCTG
      901 GAGCAGGCGG CAGTGCCGGA AGTGGCGCTG GAGGGTCTGC CGGATCTGGA GCCGGTGGTT
      961 CTGCCGGCTC CGGCGCTGGT GGGAGCGCTT CCGGAAGTAG TCCCGGTTCT GGGAGTGGCG
     1021 GTAGTGGTGC TGGAGGCAGC GCAGGCTCTG GAGCAGGCGG CAGTGCCGGA AGTGGCGCTG
     1081 GAGGGTCTGC CGGATCTGGA GCCGGTGGTT CTGCCGGCTC CGGCGCTGGT GGGAGCGCTT
     1141 CCGGAAGTAG TCCCGGTAAT TCCGCTGACG GCGGCGGAGG ATCGGGTGGT AGTGGTGGTT
     1201 CAGGAGGAGG ATCGACCCAA GGAGGATCCA TGGTGAGCGT GATCAAGCCC GAGATGAAGA
     1261 TCAAGCTGTG CATGAGGGGC ACCGTGAACG GCCACAACTT CGTGATCGAG GGCGAGGGCA
     1321 AGGGCAACCC CTACGAGGGC ACCCAGATCC TGGACCTGAA CGTGACCGAG GGCGCCCCCC
     1381 TGCCCTTCGC CTACGACATC CTGACCACCG TGTTCCAGTA CGGCAACAGG GCCTTCACCA
     1441 AGTACCCCGC CGACATCCAG GACTACTTCA AGCAGACCTT CCCCGAGGGC TACCACTGGG
     1501 AGAGGAGCAT GACCTACGAG GACCAGGGCA TCTGCACCGC CACCAGCAAC ATCAGCATGA
     1561 GGGGCGACTG CTTCTTCTAC GACATCAGGT TCGACGGCGT GAACTTCCCC CCCAACGGCC
     1621 CCGTGATGCA GAAGAAGACC CTGAAGTGGG AGCCCAGCAC CGAGAAGATG TACGTGAGGG
     1681 ACGGCGTGCT GAAGGGCGAC GTGAACATGG CCCTGCTGCT GGAGGGCGGC GGCCACTACA
     1741 GGTGCGACTT CAAGACCACC TACAAGGCCA AGAAGGACGT GAGGCTGCCC GACTACCACT
     1801 TCGTGGACCA CAGGATCGAG ATCCTGAAGC ACGACAAGGA CTACAACAAG GTGAAGCTGT
     1861 ACGAGAACGC CGTGGCCAGG TACAGCATGC TGCCCAGCCA GGCCAAGGGT ACCGGAACTG
     1921 CAGCAGAGAA TTCGGGAAAC TCGAGAACAA AGCTTATGAT CCTCTGGCAT GAGATGTGGC
     1981 ATGAAGGCCT GGAAGAGGCA TCTCGTTTGT ACTTTGGGGA AAGGAACGTG AAAGGCATGT
     2041 TTGAGGTGCT GGAGCCCTTG CATGCTATGA TGGAACGGGG CCCCCAGACT CTGAAGGAAA
     2101 CATCCTTTAA TCAGGCCTAT GGTCGAGATT TAATGGAGGC CCAAGAGTGG TGCAGGAAGT
     2161 ACATGAAATC AGGGAATGTC AAGGACCTCC TCCAAGCCTG GGACCTCTAT TATCATGTGT
     2221 TCCGACGAAT CTCAAAGCCC GGGGGTAGTG GTGCTGGCTC TGGTGCTGGT AGTGGCGCTG
     2281 GTGAAAACCT GTATTTTCAG CTGGTCGATG GTGGTAGTGC TGGTGGTTCC GGAGGTGTCG
     2341 ACatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg
     2401 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     2461 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     2521 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     2581 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     2641 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     2701 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     2761 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     2821 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga
     2881 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     2941 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     3001 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aatAGATCCG
     3061 GCTCTTACCC ATACGATGTT CCAGATTACG CTAGATCTca gatgaggctg cctagtgctg
     3121 atgtttatag atttgcagag cctgactctg aagagaatat tatatttgaa gagaacatgc
     3181 agcccaaggc tggaattcca attatcaaag caggaactgt tattaaactt atagagaggc
     3241 ttacgtacca tatgtacgca gatcccaatt ttgttcggac atttcttaca acatacagat
     3301 ccttttgcaa acctcaagaa ctactgagtc ttataataga aaggtttgaa attccagagc
     3361 ctgagccaac agaagctgat cgcatagcta tagagaatgg agatcaaccc ttgagtgcag
     3421 aactgaaaag atttagaaaa gaatatatac agcctgtgca actgcgagta ttaaatgtat
     3481 gtcggcactg ggtagagcac cacttctatg attttgaaag agatgcatat cttttgcaac
     3541 gaatggaaga atttattgga acagtaagag gtaaagcaat gaaaaaatgg gttgaatcca
     3601 tcactaaaat aatccaaagg aaaaaaattg caagagacaa tggaccaggt cataatatta
     3661 catttcagag ttcacctccc acagttgagt ggcatataag cagacctggg cacatagaga
     3721 cttttgacct gctcacctta cacccaatag aaattgctcg acaactcact ttacttgaat
     3781 cagatctata ccgagctgta cagccatcag aattagttgg aagtgtgtgg acaaaagaag
     3841 acaaagaaat taactctcct aatcttctga aaatgattcg acataccacc aacctcactc
     3901 tgtggtttga gaaatgtatt gtagaaactg aaaatttaga agaaagagta gctgtggtga
     3961 gtcgaattat tgagattcta caagtctttc aagagttgaa caactttaat ggtgtccttg
     4021 aggttgtcag tgctatgaat tcatcacctg tttacagact agaccacaca tttgagcaaa
     4081 taccaagtcg ccagaagaaa attttagaag aagctcatga attgagtgaa gatcactata
     4141 agaaatattt ggcaaaactc aggtctatta atccaccatg tgtgcctttc tttggaattt
     4201 atctcactaa tatcttgaaa acagaagaag gcaaccctga ggtcctaaaa agacatggaa
     4261 aagagcttat aaactttagc aaaaggagga aagtagcaga aataacagga gagatccagc
     4321 agtaccaaaa tcagccttac tgtttacgag tagaatcaga tatcaaaagg ttctttgaaa
     4381 acttgaatcc gatgggaaat agcatggaga aggaatttac agattatctt ttcaacaaat
     4441 ccctagaaat agaaccacga aaccctaagc ctctcccaag atttccaaaa aaatatagct
     4501 atcccctaaa atctcctggt gttcgtccat caaacccaag accaggtacc taaGATCTTA
     4561 AAAAGCGGCC GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG
     4621 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG
     4681 TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT
     4741 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
     4801 TATCTTAAGG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT
     4861 TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA
     4921 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG
     4981 AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT
     5041 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC
     5101 CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG
     5161 GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG
     5221 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT
     5281 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG
     5341 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT
     5401 CCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG
     5461 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG
     5521 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG
     5581 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC
     5641 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG
     5701 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG
     5761 ATCGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA
     5821 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
     5881 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC
     5941 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG
     6001 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG
     6061 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT
     6121 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
     6181 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA
     6241 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA
     6301 CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC
     6361 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT
     6421 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT
     6481 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC
     6541 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG
     6601 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG
     6661 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
     6721 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA
     6781 CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA
     6841 AAACGCACGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT
     6901 CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC
     6961 CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG
     7021 GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT
     7081 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC
     7141 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     7201 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     7261 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     7321 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     7381 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     7441 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     7501 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     7561 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     7621 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     7681 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     7741 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     7801 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT
     7861 CCCCTGATTC TGTGGATAAC CGTATTACCG CCATGCATTA GTTATTAATA GTAATCAATT
     7921 ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCG
//