LOCUS       pCAGGS-PB1-MR-LOV2      6753 bp ds-DNA     circular     26-OCT-2021
DEFINITION  .
KEYWORDS    "Creator:Masaru Yoshikawa" "Deposit:BOX30-8D"                      
FEATURES             Location/Qualifiers
     misc_feature    1..1712
                     /label="CAG promotor"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        12..324
                     /label="CMV immediate early enhancer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        78..365
                     /label="CAG enhancer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1740..3566
                     /label="Translation 1740-3566"
     CDS             1743..2045
                     /label="PB1-p62(Ash)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             2427..3089
                     /label="Monti-Red"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3138..3563
                     /label="LOV2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3767..4231
                     /label="Rabbit b-globin 3'-flanking sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4246..4266)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(4272..4294)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(4298..4328)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(4979..5661)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(5759..6418)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
       61 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      121 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      181 tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca gtacatcaag
      241 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      301 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      361 tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
      421 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
      481 ggggcggggg gggggggggc gcgcgccagg cggggcgggg cgggggcgag ggcggggcgg
      541 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      601 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      661 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct
      721 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      781 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa
      841 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg
      901 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg
      961 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc
     1021 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     1081 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc
     1141 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg
     1201 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     1261 cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag
     1321 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     1381 ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     1441 gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     1501 tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttgttgt gctgtctcat cattttggca aagaattcat agtaGCTAGC ATTGCCACCA
     1741 TGGCGTCGCT CACCGTGAAG GCCTACCTTC TGGGCAAGGA GGACGCGGCG CGCGAGATTC
     1801 GCCGCTTCAG CTTCTGTTGC AGCCCCGAGC CTGAGGCGGA AGCCGAGGCT GCGGCGGGTC
     1861 CGGGACCCTG CGAGCGGCTG CTGAGCCGGG TGGCCGCCCT GTTCCCCGCG CTGCGGCCTG
     1921 GCGGCTTCCA GGCGCACTAC CGCGATGAGG ACGGGGACTT GGTTGCCTTT TCCAGTGACG
     1981 AGGAATTGAC AATGGCCATG TCCTACGTGA AGGATGACAT CTTCCGAATC TACATTAAAG
     2041 AGAAAACCGG TTCTGGGAGT GGCGGTAGTG GTGCTGGAGG CAGCGCAGGC TCTGGAGCAG
     2101 GCGGCAGTGC CGGAAGTGGC GCTGGAGGGT CTGCCGGATC TGGAGCCGGT GGTTCTGCCG
     2161 GCTCCGGCGC TGGTGGGAGC GCTTCCGGAA GTAGTCCCGG TTCTGGGAGT GGCGGTAGTG
     2221 GTGCTGGAGG CAGCGCAGGC TCTGGAGCAG GCGGCAGTGC CGGAAGTGGC GCTGGAGGGT
     2281 CTGCCGGATC TGGAGCCGGT GGTTCTGCCG GCTCCGGCGC TGGTGGGAGC GCTTCCGGAA
     2341 GTAGTCCCGG TAATTCCGCT GACGGCGGCG GAGGATCGGG TGGTAGTGGT GGTTCAGGAG
     2401 GAGGATCGAC CCAAGGAGGA TCCATGGTGG GCGTGATCGC CAAGCAGATG ACCTACAAGG
     2461 TGTACATGAG CGGCACCGTG AACGGCCACT ACTTCGAGGT GGAGGGCGAC GGCAAGGGCA
     2521 AGCCCTACGA GGGCGAGCAG ACCGTGAGGC TGACCGTGAC CAAGGGTGGC CCCCTGCCCT
     2581 TCGCCTGGGA CATCCTGAGC CCCCAGCTCA TGTACGGCAG CATCACCTTC ACCAAGTACC
     2641 CCGAGGACAT CCCCGACTAC TTCAAACAGA GCTTCCCCGA GGGCTTCACC TGGGAGCGCA
     2701 TCATGAACTT CGAGGACGGC GCCGTGTGCA CCGTGAGCAA CGACAGCAGC ATCCAGGGCA
     2761 ACTGCTTCAT CTACAACGTG AAGATCAGCG GCGTGAACTT CCCCCCCAAC GGCCCCGTGA
     2821 TGCAGAAGAA GACCCAGGGC TGGGAGCCCA GCACCGAGCG CCTGTTCGCC CGCGACGGAA
     2881 TGCTGATCGG CTACAACTAC ATGGCCCTGA AGCTGGAGGG CGGCGGCCAC TACCTGTGCG
     2941 AGTTCAAGAC CACCTACAAG GCCAAGAAGC CCGTGAGGAT GCCCGGCTTC CACTTCATCG
     3001 ACCGCAAGCT GGACGTGACC AGCCACAACC GCGACTACAC CAGCGTGGAG CAGTGCGAGA
     3061 TCGCCATCGC CCGCCACAGC CTGCTGGGCG GTACCGGAAC TGCAGCAGAG AATTCGGGAA
     3121 ACTCGAGAAC AAAGCTTgct actacacttg aacgtattga gaagaacttt gtcattactg
     3181 acccaagatt gccagataat cccattatat tcgcgtccga tagtttcttg cagttgacag
     3241 aatatagccg tgaagaaatt ttgggaagaa actgcaggtt tctacaaggt cctgaaactg
     3301 atcgcgcgac agtgagaaaa attagagatg ccatagataa ccaaacagag gtcactgttc
     3361 agctgattaa ttatacaaag agtggtaaaa agttctggaa cctctttcac ttgcagccta
     3421 tgcgagatca gaagggagat gtccagtact ttattggggt tcagttggat ggaactgagc
     3481 atgtccgaga tgctgccgag agagagggag tcatgctgat taagaaaact gcagaaaata
     3541 ttgatgaggc ggcaaaagaa ctttaaCCCG GGTAAGCGGC CGCcaccgcg gtggagctcc
     3601 agcttttgtt ccctttAGTG agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     3661 tttccTGTGT GAAATTGTTA TCCGCTaatt cactcctcag gtgcaggctg cctatcagaa
     3721 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgaGATC TTTTTCCCTC
     3781 TGCCAAAAAT TATGGGGACA TCATGAAGCC CCTTGAGCAT CTGACTTCTG GCTAATAAAG
     3841 GAAATTTATT TTCATTGCAA TAGTGTGTTG GAATTTTTTG TGTCTCTCAC TCGGAAGGAC
     3901 ATATGGGAGG GCAAATCATT TAAAACATCA GAATGAGTAT TTGGTTTAGA GTTTGGCAAC
     3961 ATATGCCCAT ATGCTGGCTG CCATGAACAA AGGTTGGCTA TAAAGAGGTC ATCAGTATAT
     4021 GAAACAGCCC CCTGCTGTCC ATTCCTTATT CCATAGAAAA GCCTTGACTT GAGGTTAGAT
     4081 TTTTTTTATA TTTTGTTTTG TGTTATTTTT TTCTTTAACA TCCCTAAAAT TTTCCTTACA
     4141 TGTTTTACTA GCCAGATTTT TCCTCCTCTC CTGACTACTC CCAGTCATAG CTGTCCCTCT
     4201 TCTCTTATGG AGATCCCTCG ACCTGCAGCC Caagcttggc gtaatcatgg tcatagctgt
     4261 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4321 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4381 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     4441 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4501 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4561 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4621 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4681 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4741 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4801 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5161 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     5221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5401 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5641 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5701 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5761 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5821 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5881 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5941 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6001 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6061 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6121 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6181 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6241 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6301 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6361 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6421 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6481 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6541 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6601 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6661 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6721 gcacatttcc ccgaaaagtg ccacctggtc gac
//