LOCUS       pCAG-PB1-AG(Y63L)-LOV2  6765 bp ds-DNA     circular     26-OCT-2021
DEFINITION  .
KEYWORDS    "Creator:Masaru Yoshikawa"                                         
FEATURES             Location/Qualifiers
     misc_feature    1..1712
                     /label="CAG promotor"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        12..324
                     /label="CMV immediate early enhancer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        78..365
                     /label="CAG enhancer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1740..3578
                     /label="Translation 1740-3578"
     CDS             1743..2045
                     /label="PB1-p62(Ash)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             2427..3101
                     /label="Azami-Green (Y63L)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3150..3575
                     /label="LOV2 (404-546)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3779..4243
                     /label="Rabbit b-globin 3'-flanking sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4258..4278)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(4284..4306)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(4310..4340)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(4991..5673)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(5771..6430)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
       61 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      121 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      181 tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca gtacatcaag
      241 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      301 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      361 tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
      421 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
      481 ggggcggggg gggggggggc gcgcgccagg cggggcgggg cgggggcgag ggcggggcgg
      541 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      601 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      661 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct
      721 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      781 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa
      841 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg
      901 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg
      961 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc
     1021 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     1081 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc
     1141 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg
     1201 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     1261 cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag
     1321 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     1381 ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     1441 gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     1501 tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttgttgt gctgtctcat cattttggca aagaattcat agtaGCTAGC ATTGCCACCA
     1741 TGGCGTCGCT CACCGTGAAG GCCTACCTTC TGGGCAAGGA GGACGCGGCG CGCGAGATTC
     1801 GCCGCTTCAG CTTCTGTTGC AGCCCCGAGC CTGAGGCGGA AGCCGAGGCT GCGGCGGGTC
     1861 CGGGACCCTG CGAGCGGCTG CTGAGCCGGG TGGCCGCCCT GTTCCCCGCG CTGCGGCCTG
     1921 GCGGCTTCCA GGCGCACTAC CGCGATGAGG ACGGGGACTT GGTTGCCTTT TCCAGTGACG
     1981 AGGAATTGAC AATGGCCATG TCCTACGTGA AGGATGACAT CTTCCGAATC TACATTAAAG
     2041 AGAAAACCGG TTCTGGGAGT GGCGGTAGTG GTGCTGGAGG CAGCGCAGGC TCTGGAGCAG
     2101 GCGGCAGTGC CGGAAGTGGC GCTGGAGGGT CTGCCGGATC TGGAGCCGGT GGTTCTGCCG
     2161 GCTCCGGCGC TGGTGGGAGC GCTTCCGGAA GTAGTCCCGG TTCTGGGAGT GGCGGTAGTG
     2221 GTGCTGGAGG CAGCGCAGGC TCTGGAGCAG GCGGCAGTGC CGGAAGTGGC GCTGGAGGGT
     2281 CTGCCGGATC TGGAGCCGGT GGTTCTGCCG GCTCCGGCGC TGGTGGGAGC GCTTCCGGAA
     2341 GTAGTCCCGG TAATTCCGCT GACGGCGGCG GAGGATCGGG TGGTAGTGGT GGTTCAGGAG
     2401 GAGGATCGAC CCAAGGAGGA TCCATGGTGA GCGTGATCAA GCCCGAGATG AAGATCAAGC
     2461 TGTGCATGAG GGGCACCGTG AACGGCCACA ACTTCGTGAT CGAGGGCGAG GGCAAGGGCA
     2521 ACCCCTACGA GGGCACCCAG ATCCTGGACC TGAACGTGAC CGAGGGCGCC CCCCTGCCCT
     2581 TCGCCTACGA CATCCTGACC ACCGTGTTCC AGCTGGGCAA CAGGGCCTTC ACCAAGTACC
     2641 CCGCCGACAT CCAGGACTAC TTCAAGCAGA CCTTCCCCGA GGGCTACCAC TGGGAGAGGA
     2701 GCATGACCTA CGAGGACCAG GGCATCTGCA CCGCCACCAG CAACATCAGC ATGAGGGGCG
     2761 ACTGCTTCTT CTACGACATC AGGTTCGACG GCGTGAACTT CCCCCCCAAC GGCCCCGTGA
     2821 TGCAGAAGAA GACCCTGAAG TGGGAGCCCA GCACCGAGAA GATGTACGTG AGGGACGGCG
     2881 TGCTGAAGGG CGACGTGAAC ATGGCCCTGC TGCTGGAGGG CGGCGGCCAC TACAGGTGCG
     2941 ACTTCAAGAC CACCTACAAG GCCAAGAAGG ACGTGAGGCT GCCCGACTAC CACTTCGTGG
     3001 ACCACAGGAT CGAGATCCTG AAGCACGACA AGGACTACAA CAAGGTGAAG CTGTACGAGA
     3061 ACGCCGTGGC CAGGTACAGC ATGCTGCCCA GCCAGGCCAA GGGTACCGGA ACTGCAGCAG
     3121 AGAATTCGGG AAACTCGAGA ACAAAGCTTg ctactacact tgaacgtatt gagaagaact
     3181 ttgtcattac tgacccaaga ttgccagata atcccattat attcgcgtcc gatagtttct
     3241 tgcagttgac agaatatagc cgtgaagaaa ttttgggaag aaactgcagg tttctacaag
     3301 gtcctgaaac tgatcgcgcg acagtgagaa aaattagaga tgccatagat aaccaaacag
     3361 aggtcactgt tcagctgatt aattatacaa agagtggtaa aaagttctgg aacctctttc
     3421 acttgcagcc tatgcgagat cagaagggag atgtccagta ctttattggg gttcagttgg
     3481 atggaactga gcatgtccga gatgctgccg agagagaggg agtcatgctg attaagaaaa
     3541 ctgcagaaaa tattgatgag gcggcaaaag aactttaaCC CGGGTAAGCG GCCGCcaccg
     3601 cggtggagct ccagcttttg ttccctttAG TGagggttaa ttgcgcgctt ggcgtaatca
     3661 tggtcatagc tgtttccTGT GTGAAATTGT TATCCGCTaa ttcactcctc aggtgcaggc
     3721 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaGA
     3781 TCTTTTTCCC TCTGCCAAAA ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC
     3841 TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC
     3901 ACTCGGAAGG ACATATGGGA GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA
     3961 GAGTTTGGCA ACATATGCCC ATATGCTGGC TGCCATGAAC AAAGGTTGGC TATAAAGAGG
     4021 TCATCAGTAT ATGAAACAGC CCCCTGCTGT CCATTCCTTA TTCCATAGAA AAGCCTTGAC
     4081 TTGAGGTTAG ATTTTTTTTA TATTTTGTTT TGTGTTATTT TTTTCTTTAA CATCCCTAAA
     4141 ATTTTCCTTA CATGTTTTAC TAGCCAGATT TTTCCTCCTC TCCTGACTAC TCCCAGTCAT
     4201 AGCTGTCCCT CTTCTCTTAT GGAGATCCCT CGACCTGCAG CCCaagcttg gcgtaatcat
     4261 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4321 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4381 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     4441 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4501 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4561 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4621 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4681 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4741 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     4801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     5221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     5461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6361 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6721 taggggttcc gcgcacattt ccccgaaaag tgccacctgg tcgac
//