LOCUS       Exported                4093 bp ds-DNA     circular SYN 07-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Nov 7, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        197..1365
                     /label=EF1a P
                     /note="EF1 alpha promoter"
                     /note="color: #8080ff"
     CDS             1386..2027
                     /codon_start=1
                     /label=cas5
                     /note="color: #993366"
                     /translation="MRFILEISGDLACFTRSELKVERVSYPVITPSAARNILMAILWKP
                     AIRWKVLKIEILKPIQWTNIRRNEVGTKMSERSGSLYIEDNRQQRASMLLKDVAYRIHA
                     DFDMTSEAGESDNYVKFAEMFKRRAKKGQYFHQPYLGCREFPCDFRLLEKAEDGLPLED
                     ITQDFGFMLYDMDFSKSDPRDSNNAEPMFYQCKAVNGVITVPPADSEEVKR"
     CDS             2040..2066
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             2067..2087
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    2091..2109
                     /label=BGH polyA
                     /note="BGH poly A signal (Bovine growth hormone)"
                     /note="color: #8000ff"
     polyA_signal    2110..2296
                     /label=BGH polyA
                     /note="BGH poly A signal (Bovine growth hormone)"
                     /note="color: #8000ff"
     misc_feature    2306..4093
                     /label=pSmart-Kan HC backbone
                     /note="pSmart-Kan HC backbone"
                     /note="color: #ff8000"
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
      241 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
      301 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
      361 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
      421 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
      481 gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc
      541 tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc
      601 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
      661 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
      721 ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg
      781 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
      841 gcgagcgcgg ccaccgagaa tcggacgggg gtcggacggg ggtagtctca agctggccgg
      901 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
      961 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctccaggg
     1021 ggctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1081 aaaggggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1141 tccaggcacc tcgattagtt ctggagcttt tggagtacgt cgtctttagg ttggggggag
     1201 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1261 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1321 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaacaccg
     1381 ccaccatgcg gttcatcctg gaaatcagcg gcgacctggc ctgcttcaca agaagcgagc
     1441 tgaaggtcga gcgggtgtca taccctgtga tcacccctag cgccgccaga aacatcctga
     1501 tggccattct gtggaagccc gccatcagat ggaaggtgct gaagatcgag atcctgaagc
     1561 ctatccagtg gaccaacatc cggcggaacg aagtgggcac caagatgagc gagagaagcg
     1621 gcagcctgta catcgaggac aacagacagc agcgggcctc catgctgctg aaggatgtgg
     1681 cctatagaat ccacgccgac ttcgacatga caagcgaggc cggcgagagc gacaactacg
     1741 tgaagttcgc cgagatgttc aagcggagag ccaagaaggg ccagtacttc caccagcctt
     1801 acctgggctg cagagagttc ccctgcgact tcagactgct ggaaaaggcc gaggatggcc
     1861 tgcctctgga agatatcacc caggacttcg gcttcatgct gtacgacatg gacttcagca
     1921 agagcgaccc cagagacagc aacaacgccg agcctatgtt ctaccagtgc aaggccgtga
     1981 acggcgtgat cactgtgcct ccagccgata gcgaggaagt gaagagaggc agcgtcggct
     2041 acccctacga tgtgcctgat tacgccccta agaaaaagcg gaaagtgtga ctcgctgatc
     2101 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     2161 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     2221 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     2281 ggaggattgg gaagacaata gcaggaacga attcaagctt gatatcattc aggacgagcc
     2341 tcagactcca gcgtaactgg actgcaatca actcactggc tcaccttcac gggtgggcct
     2401 ttcttcggta gaaaatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2521 caactctttt tccgaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2941 tcctgtcggg tttcgccacc tctgacttga gcatcgattt ttgtgatgct cgtcaggggg
     3001 gcggagccta tggaaaaacg ccagcaacgc agaaaggccc acccgaaggt gagccaggtg
     3061 attacatttg ggccctcatt agaaaaactc atcgagcatc aagtgaaact gcaatttatt
     3121 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     3181 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     3241 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     3301 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca
     3361 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcac caaccaaacc
     3421 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgccgt taaaaggaca
     3481 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     3541 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt
     3601 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     3661 aaattccgtc agccagttta gcctgaccat ctcatctgta acatcattgg caacgctacc
     3721 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     3781 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     3841 gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcattttagc
     3901 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     3961 attatggtga aagttggaac ctcttacgtg ccgatcaagt caaaagcctc cggtcggagg
     4021 cttttgactt tctgctatgg aggtcaggta tgatttaaat ggtcagtatt gagcgatatc
     4081 tagagaattc gtc
//
