LOCUS       Exported                4300 bp ds-DNA     circular SYN 07-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Nov 14, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        197..1365
                     /label=EF1a P
                     /note="EF1 alpha promoter"
                     /note="color: #8080ff"
     CDS             1386..2234
                     /codon_start=1
                     /label=cas7
                     /note="color: #993366"
                     /translation="MTIEKRYDFVFLFDVQDGNPNGDPDAGNLPRIDPQTGEGLVTDVC
                     LKRKVRNFIQMTQNDEHHDIFIREKGILNNLIDEAHEQENVKGKEKGEKTEAARQYMCS
                     RYYDIRTFGAVMTTGKNAGQVRGPVQLTFSRSIDPIMTLEHSITRMAVTNEKDASETGD
                     NRTMGRKFTVPYGLYRCHGFISTHFAKQTGFSENDLELFWQALVNMFDHDHSAARGQMN
                     ARGLYVFEHSNNLGDAPADSLFKRIQVVKKDGVEVVRSFDDYLVSVDDKNLEETKLLRK
                     LG"
     CDS             2247..2273
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             2274..2294
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    2298..2316
                     /label=BGH polyA
                     /note="BGH poly A signal (Bovine growth hormone)"
                     /note="color: #8000ff"
     polyA_signal    2317..2503
                     /label=BGH polyA
                     /note="BGH poly A signal (Bovine growth hormone)"
                     /note="color: #8000ff"
     misc_feature    2513..4300
                     /label=pSmart-Kan HC backbone
                     /note="pSmart-Kan HC backbone"
                     /note="color: #ff8000"
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
      241 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
      301 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
      361 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
      421 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
      481 gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc
      541 tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc
      601 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
      661 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
      721 ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg
      781 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
      841 gcgagcgcgg ccaccgagaa tcggacgggg gtcggacggg ggtagtctca agctggccgg
      901 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
      961 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctccaggg
     1021 ggctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1081 aaaggggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1141 tccaggcacc tcgattagtt ctggagcttt tggagtacgt cgtctttagg ttggggggag
     1201 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1261 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1321 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaacaccg
     1381 ccaccatgac catcgagaag cgctacgact tcgtgttcct gttcgacgtg caagacggca
     1441 accccaacgg cgatcctgat gccggaaacc tgcctagaat cgaccctcag acaggcgagg
     1501 gcctcgtgac agatgtgtgc ctgaagcgga aagtgcggaa cttcatccag atgacccaga
     1561 acgacgagca ccacgacatc ttcatcagag agaagggcat cctgaacaac ctgatcgacg
     1621 aggcccacga gcaagagaac gtgaagggca aagagaaagg cgagaaaacc gaggccgcca
     1681 gacagtacat gtgcagccgg tactacgaca tcagaacctt cggcgccgtg atgaccaccg
     1741 gcaagaatgc tggacaagtg cggggacctg tgcagctgac cttcagcaga tccatcgatc
     1801 ccatcatgac cctggaacac agcatcacca gaatggccgt gaccaatgag aaggacgcca
     1861 gcgaaaccgg cgacaacaga accatgggca gaaagttcac cgtgccttac ggcctgtacc
     1921 ggtgccacgg ctttatcagc acccacttcg ccaagcagac cggcttcagc gagaacgacc
     1981 tggaactgtt ttggcaggcc ctggtcaaca tgttcgatca cgatcactct gccgccagag
     2041 gccagatgaa tgccagagga ctgtacgtgt tcgagcacag caacaacctg ggagatgccc
     2101 ctgccgacag cctgttcaag agaatccagg tggtcaagaa agacggcgtg gaagtcgtgc
     2161 ggagcttcga cgattacctg gtgtccgtgg acgacaagaa cctggaagag acaaagctgc
     2221 tgcggaagct cggcggctct gtgggctatc cttacgacgt gccagactac gcccctaaga
     2281 aaaagcgcaa agtgtgactc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     2341 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2401 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     2461 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggaacgaatt
     2521 caagcttgat atcattcagg acgagcctca gactccagcg taactggact gcaatcaact
     2581 cactggctca ccttcacggg tgggcctttc ttcggtagaa aatcaaagga tcttcttgag
     2641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaggtaactg gcttcagcag
     2761 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2821 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2881 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2941 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3001 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3061 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3121 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagca
     3181 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcaga
     3241 aaggcccacc cgaaggtgag ccaggtgatt acatttgggc cctcattaga aaaactcatc
     3301 gagcatcaag tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     3361 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     3421 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     3481 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     3541 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     3601 atcaaaatca ctcgcaccaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     3661 aaatacgcga tcgccgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     3721 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     3781 gaatgctgtt ttccctggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     3841 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagcc tgaccatctc
     3901 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     3961 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     4021 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
     4081 ttcccgttga atatggctca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     4141 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     4201 atcaagtcaa aagcctccgg tcggaggctt ttgactttct gctatggagg tcaggtatga
     4261 tttaaatggt cagtattgag cgatatctag agaattcgtc
//
